=== Methods ===

=== Methods ===

==== Minimal Inhibitory Concentration (MIC) ====

==== Minimal Inhibitory Concentration ====

* Minimal Inhibitory Concentrations (MIC) were determined, using the EUCAST broth microdilution protocol, for the following antimicrobials:

* Minimal Inhibitory Concentrations (MIC) were determined, using the EUCAST broth microdilution protocol, for the following antimicrobials:

==== Flow Cytometry ====  

==== Flow Cytometry ====  

* After being resuspended in physiological saline, both treated and untreated biofilms (again, both prepared as described in the microarray methods) were stained with an ROS-specific dye, and the passed through a Cyan ADP flow cytometer for quantification.

* After being resuspended in physiological saline, both treated and untreated ''B. cenocepacia'' J2315 biofilms (again, both prepared as described in the microarray methods) were stained with an ROS-specific dye, and the passed through a Cyan ADP flow cytometer for quantification.

** Biofilms were dyed by incubating for 30 minutes in the dark, in the presence of the dye.

** Biofilms were dyed by incubating for 30 minutes in the dark, in the presence of the dye.

* Data for at least 50,000 cells was collected in each sample.

* Data for at least 50,000 cells was collected in each sample.

==== Inhibition of SOD, ICL and SDH ====

==== Inhibition of SOD, ICL and SDH ====

* To evaluate the importance of superoxide dismutase (SOD), isocitrate lyase (ICL) and succinate dehydrogenase (SDH), biofilm persister cells (presumably prepared as described above, though not explicitly stated in the article) were quantified after exposure to 4x MIC tobramycin in conjunction with the following inhibitory agents:

* To evaluate the importance of superoxide dismutase (SOD), isocitrate lyase (ICL) and succinate dehydrogenase (SDH), ''B. cenocepacia'' J2315 biofilm persister cells (presumably prepared as described above, though not explicitly stated in the article) were quantified after exposure to 4x MIC tobramycin in conjunction with the following inhibitory agents:

** Diethyldithiocarbamate, inhibiting SOD.

** Diethyldithiocarbamate, inhibiting SOD.

** Itaconate or 3-NP, both inhibiting ICL.

** Itaconate or 3-NP, both inhibiting ICL.

** 2-thenoyltrifluoracetone, inhibiting SDH.

** 2-thenoyltrifluoracetone, inhibiting SDH.