|
|
| Line 2: |
Line 2: |
| | 3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5' | | 3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5' |
| | | | |
| − | Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.
| + | Reading Frame 1: |
| − | 5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
| + | Reading Frame 2: |
| − | There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
| + | Reading Frame 3: |
| − | In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
| + | Reading Frame 4: |
| − | The genetic code is read in the 5’ to 3’ direction.
| + | Reading Frame 5: |
| − | Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
| + | Reading Frame 6: |
| − | Wiki Hint: if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
| + | |
| − | Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
| + | |
| − | By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.
| + | |
| | | | |
| | | | |
| | {{Template: Jwoodlee}} | | {{Template: Jwoodlee}} |
Revision as of 21:10, 12 September 2015
5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3'
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'
Reading Frame 1:
Reading Frame 2:
Reading Frame 3:
Reading Frame 4:
Reading Frame 5:
Reading Frame 6:
BIOL 367, Fall 2015, User Page, Team Page
| Weekly Assignments
|
Individual Journal Pages
|
Shared Journal Pages
|
|
|
|
|
