Difference between revisions of "Msaeedi23 Week 2"

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  5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
 
  5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
 +
3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’
  
 
* There are six possible reading frames in double-stranded DNA.  Using the [http://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table genetic code], translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
 
* There are six possible reading frames in double-stranded DNA.  Using the [http://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table genetic code], translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
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** The genetic code is read in the 5’ to 3’ direction.
 
** The genetic code is read in the 5’ to 3’ direction.
 
** Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
 
** Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
 +
*proceeding the N-ter-, - will be used in place of a stop codon
 +
* +1
 +
RNA: 5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
 +
Protein: N-ter-RMLIPCSAYNPAASSAGGIL-C-ter
 +
* +2
 +
RNA: 5’-guaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuu-3’
 +
Protein: N-ter-VC-YHVPRITQPPVPLAAF-C-ter
 +
* +3
 +
RNA: 5’-uaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuu-3’
 +
Protein: N-ter-YANTMFRV-PSRQFRWRHF-C-ter
 +
* -1
 +
RNA: 5’-uaaaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauacg-3’
 +
Protein: N-ter--NAASGTGGWVIRGTWY-HT-C-ter
 +
* -2
 +
RNA: 5’-aaaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcaua-3’
 +
Protein: N-ter-KMPPAELAAGLYAEHGISI-C-ter
 +
* -3
 +
RNA: 5’-aaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauac-3’
 +
Protein: N-ter-KCRQRNWRLGYTRNMVLAY-C-ter
 
* Wiki Hint:  if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
 
* Wiki Hint:  if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
 
* Which of the reading frames (if any) of the reading frames you translated is an ''open reading frame'', i.e., does not contain a stop codon?
 
* Which of the reading frames (if any) of the reading frames you translated is an ''open reading frame'', i.e., does not contain a stop codon?
 +
**frames +1, -2, and -3.
 
** By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.
 
** By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.
 +
 +
{{template: msaeedi23}}

Latest revision as of 03:41, 15 September 2015

Individual Journal Assignment

The Genetic Code

  • Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.
5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’
  • There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
    • In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
    • The genetic code is read in the 5’ to 3’ direction.
    • Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
  • proceeding the N-ter-, - will be used in place of a stop codon
  • +1
RNA: 5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
Protein: N-ter-RMLIPCSAYNPAASSAGGIL-C-ter
  • +2
RNA: 5’-guaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuu-3’
Protein: N-ter-VC-YHVPRITQPPVPLAAF-C-ter
  • +3
RNA: 5’-uaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuu-3’
Protein: N-ter-YANTMFRV-PSRQFRWRHF-C-ter
  • -1
RNA: 5’-uaaaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauacg-3’
Protein: N-ter--NAASGTGGWVIRGTWY-HT-C-ter
  • -2
RNA: 5’-aaaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcaua-3’
Protein: N-ter-KMPPAELAAGLYAEHGISI-C-ter
  • -3
RNA: 5’-aaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauac-3’
Protein: N-ter-KCRQRNWRLGYTRNMVLAY-C-ter
  • Wiki Hint: if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
  • Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
    • frames +1, -2, and -3.
    • By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.

Mahrad Saeedi

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