Difference between revisions of "Bklein7 Week 2"

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(Created headings, invoked template, and copied over the assignment)
 
(Wrote out the complementary DNA & associated mRNA strands)
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  5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
 
  5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
−
 
+
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'
 +
   
 
* There are six possible reading frames in double-stranded DNA.  Using the [http://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table genetic code], translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
 
* There are six possible reading frames in double-stranded DNA.  Using the [http://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table genetic code], translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
 
** In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
 
** In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
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* Which of the reading frames (if any) of the reading frames you translated is an ''open reading frame'', i.e., does not contain a stop codon?
 
* Which of the reading frames (if any) of the reading frames you translated is an ''open reading frame'', i.e., does not contain a stop codon?
 
** By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.
 
** By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.
 +
 +
*mRNA strands
 +
 +
5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
 +
3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5'
  
 
==Links==
 
==Links==

Revision as of 05:37, 14 September 2015

Individual Journal Assignment

The Genetic Code

  • Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.
5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'
  • There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
    • In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
    • The genetic code is read in the 5’ to 3’ direction.
    • Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.
  • Wiki Hint: if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
  • Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
    • By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.
  • mRNA strands
5’-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’
3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5'

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