Difference between revisions of "Kevin Wyllie Week 2"

Revision as of 05:48, 15 September 2015

Journal Week 2

See my protocol for this assignment on my user page.

The given DNA sequence is written in the top strand, while its complementary strand is shown below it.

5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta- 3'
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat- 5'

Reading frames:

• +1
  • Transcribed: 5'- cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gct ggc ggc auu uua -3'
  • Translated: Nter-R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L-Cter (This is an open reading frame.)
• +2
  • Transcribed: 5'- gua ugc uaa uac cat guu ccg cgu ata acc cag ccg cca guu ccg ctg gcg gca uuu -3'
  • Translated: Nter-V-C-Cter
• +3
  • Transcribed: 5'- uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cat uuu -3'
  • Translated: Nter-Y-A-N-T-M-F-R-V-Cter
• -1
  • Transcribed: 5'- uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga aca ugg uau uag cau acg -3'
  • Translated: The first codon in this reading frame is a STOP codon, so there is no polypeptide.
• -2
  • Transcribed: 5'- aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cau ggu auu agc aua -3'
  • Translated: Nter-K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I-Cter (This is an open reading frame.)
• -3
  • Transcribed: 5'- aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac aug gua uua gca uac -3'
  • Translated: Nter-K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y-Cter (This is an open reading frame.)

• User:Kwyllie