| | * The stop codon indicates a new challenge. Following the start codon, the sequence must be read in groups of three. As a result, I must space out each of the codons before making the search to ensure that the stop codon is in the same reading frame as the start codon. To do this, I began with putting a newline after the end tag of the start codon in the way to how I added a newline after the rbs. Then, I knew I needed to space each codon. Having used this command in the [[Nanguiano_Week_3 | week 3 assignment]], I knew exactly what command to use: <code>sed "s/.../& /g"</code>, only this time I would include a 5 before the s to indicate that it needs to search the fifth line. There are three possibilities as to what the stop codon can be: tga, tag, or taa. This requires me to use an or command that can search for not just one possibility, but for many. The way this is done is by putting a "|" character in between possibilities. So I could search the fifth line for <code>tga|tag|taa</code> in sed after passing in the parameter -r. Then, I would need to remove the spaces between the codons, and put spaces between the tags. The command that I created was as follows: | | * The stop codon indicates a new challenge. Following the start codon, the sequence must be read in groups of three. As a result, I must space out each of the codons before making the search to ensure that the stop codon is in the same reading frame as the start codon. To do this, I began with putting a newline after the end tag of the start codon in the way to how I added a newline after the rbs. Then, I knew I needed to space each codon. Having used this command in the [[Nanguiano_Week_3 | week 3 assignment]], I knew exactly what command to use: <code>sed "s/.../& /g"</code>, only this time I would include a 5 before the s to indicate that it needs to search the fifth line. There are three possibilities as to what the stop codon can be: tga, tag, or taa. This requires me to use an or command that can search for not just one possibility, but for many. The way this is done is by putting a "|" character in between possibilities. So I could search the fifth line for <code>tga|tag|taa</code> in sed after passing in the parameter -r. Then, I would need to remove the spaces between the codons, and put spaces between the tags. The command that I created was as follows: |
Exception encountered, of type "Error"