Difference between revisions of "Troque Week 8"

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(Starting the Individual Journal)
 
m (Normalize the log ratios for the set of slides in the experiment: Updated this page, will need to edit)
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== Normalize the log ratios for the set of slides in the experiment ==
 
== Normalize the log ratios for the set of slides in the experiment ==
To scale and center the data (between chip normalization) perform the following operations:
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To scale and center the data (between chip normalization) I performed the following operations:
  
* Insert a new Worksheet into your Excel file, and name it "scaled_centered".
+
* Insert a new Worksheet into my Excel file, and named it "scaled_centered".
* Go back to the "compiled_raw_data" worksheet, Select All and Copy.  Go to your new "scaled_centered" worksheet, click on the upper, left-hand cell (cell A1) and Paste.
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* Go back to the "compiled_raw_data" worksheet, Select All and Copy.  I went to my new "scaled_centered" worksheet, click on the upper, left-hand cell (cell A1) and Paste.
 
* Insert two rows in between the top row of headers and the first data row.
 
* Insert two rows in between the top row of headers and the first data row.
* In cell A2, type "Average" and in cell A3, type "StdDev".
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* In cell A2, type "Average" and in cell A3, type "StDev".
* You will now compute the Average log ratio for each chip (each column of data).  In cell B2, type the following equation:
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* I then computed the Average log ratio for each chip (each column of data).  In cell B2, type the following equation:
 
  =AVERAGE(B4:B5224)
 
  =AVERAGE(B4:B5224)
 
Note: We tried to do a keyboard shortcut using CTRL + Shift + Down buttons, but row 363 has a missing data so we had to manually type in "B5224" for the end of the data.
 
Note: We tried to do a keyboard shortcut using CTRL + Shift + Down buttons, but row 363 has a missing data so we had to manually type in "B5224" for the end of the data.
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{{Template:Troque_Journal}}
 
{{Template:Troque_Journal}}

Revision as of 22:14, 15 October 2015

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Normalize the log ratios for the set of slides in the experiment

To scale and center the data (between chip normalization) I performed the following operations:

  • Insert a new Worksheet into my Excel file, and named it "scaled_centered".
  • Go back to the "compiled_raw_data" worksheet, Select All and Copy. I went to my new "scaled_centered" worksheet, click on the upper, left-hand cell (cell A1) and Paste.
  • Insert two rows in between the top row of headers and the first data row.
  • In cell A2, type "Average" and in cell A3, type "StDev".
  • I then computed the Average log ratio for each chip (each column of data). In cell B2, type the following equation:
=AVERAGE(B4:B5224)

Note: We tried to do a keyboard shortcut using CTRL + Shift + Down buttons, but row 363 has a missing data so we had to manually type in "B5224" for the end of the data.



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