*"Run MAPPFinder" was clicked and the program was allowed to complete its analysis

*"Run MAPPFinder" was clicked and the program was allowed to complete its analysis

*"Show Ranked List" was clicked to see a list of the most significant Gene Ontology terms

*"Show Ranked List" was clicked to see a list of the most significant Gene Ontology terms

*'''Top 10 Ranked GO Terms'''

*'''Top 10 Ranked GO Terms, found using the 2009 Database'''

#biopolymer biosynthetic process

#biopolymer biosynthetic process

#macromolecule biosynthetic process

#macromolecule biosynthetic process

**Thiamin was found to be a vitamin (vitamin B1), that is utilized by many organisms, including gram-negative bacteria like ''Vibrio cholerae''  

**Thiamin was found to be a vitamin (vitamin B1), that is utilized by many organisms, including gram-negative bacteria like ''Vibrio cholerae''  

**Given this UniProt result, it appears that the gene codes for a protein that binds to thiamin and employs ATP to move it across membranes (is a transmembrane protein that works via active-transport)

**Given this UniProt result, it appears that the gene codes for a protein that binds to thiamin and employs ATP to move it across membranes (is a transmembrane protein that works via active-transport)

====Working with the Results File====

===Working with the Results File===

*The results file, in the format "XXX-CriterionX-GO.txt" was copied and pasted in the folder housing all of the files related to this investigation (XXX = name given to file, CriterionX refers to the Criterion #; Criterion0 refers to the Increased criterion, Criterion1 refers to the Decreased criterion, if the Increased criterion was done first)  

*The results file, in the format "XXX-CriterionX-GO.txt" was copied and pasted in the folder housing all of the files related to this investigation (XXX = name given to file, CriterionX refers to the Criterion #; Criterion0 refers to the Increased criterion, Criterion1 refers to the Decreased criterion, if the Increased criterion was done first)  

*The "CriterionGO" file was opened via Excel in order to show the results in a tabular format  

*The "CriterionGO" file was opened via Excel in order to show the results in a tabular format  

  Percent Changed (in column L) greater than or equal to 26.5

  Percent Changed (in column L) greater than or equal to 26.5

*The top 20 GO terms in the file were searched in MAPPFinder in order to find if any relationships existed between the terms. Terms that exhibited a relationship with another term were highlighted (terms that shared a relationship were highlighted with the same color). Terms that did not have any relationships with the other top 20 terms were given bold borders

*The top 20 GO terms in the file were searched in MAPPFinder in order to find if any relationships existed between the terms. Terms that exhibited a relationship with another term were highlighted (terms that shared a relationship were highlighted with the same color). Terms that did not have any relationships with the other top 20 terms were given bold borders