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| ==Electronic Lab Notebook== | | ==Electronic Lab Notebook== |
− | Export of ''Vibrio cholerae'' GenMAPP Gene Database was used following the instructions on the [https://xmlpipedb.cs.lmu.edu/biodb/fall2015/index.php/Week_9 Week 9] assignment page. The Gene Database Testing Report template we used was found [https://xmlpipedb.cs.lmu.edu/biodb/fall2015/index.php/Gene_Database_Testing_Report_Sample here]. | + | Export of ''Vibrio cholerae'' GenMAPP Gene Database was used following the instructions on the [https://xmlpipedb.cs.lmu.edu/biodb/fall2015/index.php/Running_GenMAPP_Builder Running GenMAPP Builder] page. The Gene Database Testing Report template we used was found [https://xmlpipedb.cs.lmu.edu/biodb/fall2015/index.php/Gene_Database_Testing_Report_Sample here]. |
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| ==Export Information== | | ==Export Information== |
| | | |
− | Version of GenMAPP Builder: | + | First we downloaded UniProt XML, GOA, and GO OBO-XML files. UniProt (protein database) is linked to GO OBO-XML (Gene Ontology) through GOA (Gene Ontology Associations) through XMLPipeDB, subset GenMAPP Builder. GenMAPP Builder takes the data and puts it into PostgreSQL database, where it is converted into a GenMAPP-compatible gene database (GDB). From there, we can then download and analyze microarray data via GenMAPP. |
| + | |
| + | ===UniProt XML=== |
| + | *Clicked on the UniProt link for ''Vibrio cholerae'' serotype O1 and clicked download all with XML format as a compressed file. |
| + | *Saved <code>uniprot-organism%3A243277.xml.gz</code> in Computer T drive under new folder <code>Kzebrows</code> |
| + | *Clicked on the link to download the proteomes directory |
| + | *Downloaded file and saw that V_cholerae was downloaded 13 Oct 20016 at 07:31 |
| + | |
| + | ===GOA=== |
| + | *Followed link to Legacy Downloads |
| + | *Download obo-xml.gz to T drive Kzebrows |
| + | |
| + | *Downloaded gmbuilder-3.0 and extracted in T drive using 7 zip |
| + | |
| + | ==Create New Database in PostgreSQL== |
| + | *Launched PgAdmin III. |
| + | *Double-clicked on PostgreSQL 9.4 |
| + | *Selected "Database" and "New Database" and named it "Vcholerae_20151027_gmb3build5". Copied name to clipboard and pressed OK. |
| + | *Ran prepackaged query: open file > Thaw space > gmbuilder-3.0.0-build-5 > sql > gmbuildersql |
| + | **Query returned successfully with no results in 5697 ms |
| + | **Closed query window |
| + | |
| + | ==Configuring GenMAPP Builder to Connect to PostgreSQL Database== |
| + | *Launched gmbuilder.bat |
| + | *Select File > Configure Database |
| + | *Entered the following info and clicked OK |
| + | **Host: localhost |
| + | **Port number: 5432 |
| + | **Database name: Vcholerae_20151027_gmb3build5 |
| + | **Username: postgres |
| + | **password |
| + | *File > Import UniProt XML and found UniProt XML file |
| + | *File > Import GO OBO-XML file |
| + | |
| + | ==Exporting a GenMAPP Gene Database (.gdb)== |
| + | *Selected File > Export to database |
| + | *Typed my name into the Owner Field |
| + | *Clicked on species ''V. cholerae'' |
| + | *Created the database by saving under T drive |
| + | *Left the boxes checked for exporting all Molecule Function, Cellular Component, and Gene Ontology terms |
| + | *Clicked next to begin export process |
| + | *Start time: October 27, 2015 at 3:55 pm |
| + | |
| + | |
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| + | |
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| + | |
| + | |
| + | ==Gene Database Testing Report== |
| + | |
| + | Version of GenMAPP Builder: '''gmbuilder-3.0.0-build-5.zip |
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| Computer on which export was run: | | Computer on which export was run: |
| | | |
− | Postgres Database name: | + | Postgres Database name: '''Vcholerae_20151027_gmb3build5''' |
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| UniProt XML filename (give filename and upload and link to compressed file): | | UniProt XML filename (give filename and upload and link to compressed file): |
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