Difference between revisions of "Jwoodlee Week 2"

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(Formatted mRNA sequence)
(Setup the reading frames)
Line 3: Line 3:
  
 
* There are six possible reading frames in double-stranded DNA.  Using the [http://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table genetic code], translate all possible reading frames of this DNA sequence.
 
* There are six possible reading frames in double-stranded DNA.  Using the [http://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table genetic code], translate all possible reading frames of this DNA sequence.
  ** mRNA sequence: 5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3'
+
  mRNA sequence: 5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3'
* Wiki Hint: if you start your line with a single space character, it will appear in the grey box surrounded by the dashed line as shown above.
+
 
 +
  +1 5'- -3'
 +
+2 5'- -3'
 +
+3 5'- -3'
 +
 
 +
-1 5'- -3'
 +
-2 5'- -3'
 +
-3 5'- -3'
 +
 
 
* Which of the reading frames (if any) of the reading frames you translated is an ''open reading frame'', i.e., does not contain a stop codon?
 
* Which of the reading frames (if any) of the reading frames you translated is an ''open reading frame'', i.e., does not contain a stop codon?
 
** By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.
 
** By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.

Revision as of 21:42, 12 September 2015

5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3'
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'
  • There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence.
mRNA sequence: 5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3'
+1 5'- -3'
+2 5'- -3'
+3 5'- -3'
-1 5'- -3'
-2 5'- -3'
-3 5'- -3'
  • Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
    • By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.


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