Difference between revisions of "Jkuroda Week 2"

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(first edit)
 
(adding reading frames)
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'''Six reading frames:'''  
 
'''Six reading frames:'''  
  +1
+
  +1                       5'-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3'
5'-
+
Corresponding amino acids:  R  M  L  I  P  C  S  A  Y  N  P  A  A  S  S  A  G  G  I  L 
  
  +2
+
  +2                       5'-c gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua-3'
5'-
+
Corresponding amino acids:    V  C  STOP
  
  +3
+
  +3                       5'-cg uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a-3'
5'-
+
Corresponding amino acids:      Y  A  N  T  M  F  R  V  STOP
  
  -1
+
  -1                       5'-uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga acu agg uau uag cau acg-3'
5'-
+
Corresponding amino acids: STOP  N  A  A  S  G  T  G  G  W  V  I  R  G  T  R  Y  STOP
  
  -2
+
  -2                       5'-u aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cua ggu auu agc aua cg-3'
5'-
+
Corresponding amino acids:    K  M  P  P  A  E  L  A  A  G  L  Y  A  E  L  G  I  S  I
  
  -3
+
  -3                       5'-ua aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac uag gua uua gca uac g-3'
5'-
+
Corresponding amino acids:      K  C  R  Q  R  N  W  R  L  G  Y  T  R  N  STOP
  
___ and ___ do not contain stop codons, so they are open reading frames
+
''+1 and -2 do not contain stop codons, so they are open reading frames''
  
 
{{Template:Journal Template}}
 
{{Template:Journal Template}}

Revision as of 23:41, 14 September 2015

Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand:

Original DNA strand:
5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
Complementary DNA strand:
3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’

mRNA strand:
5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3'

Complementary mRNA strand:
5'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-3'

Six reading frames:

+1                       5'-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3'
Corresponding amino acids:   R   M   L   I   P   C   S   A   Y   N   P   A   A   S   S   A   G   G   I   L   
+2                       5'-c gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua-3'
Corresponding amino acids:     V   C   STOP
+3                       5'-cg uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a-3'
Corresponding amino acids:      Y   A   N   T   M   F   R   V   STOP
-1                       5'-uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga acu agg uau uag cau acg-3'
Corresponding amino acids: STOP  N   A   A   S   G   T   G   G   W   V   I   R   G   T   R   Y   STOP
-2                       5'-u aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cua ggu auu agc aua cg-3'
Corresponding amino acids:     K   M   P   P   A   E   L   A   A   G   L   Y   A   E   L   G   I   S   I
-3                       5'-ua aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac uag gua uua gca uac g-3'
Corresponding amino acids:      K   C   R   Q   R   N   W   R   L   G   Y   T   R   N   STOP

+1 and -2 do not contain stop codons, so they are open reading frames

Josh Kuroda's page

Individual Journal Entries

Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Week 13
Week 14
Week 15

Shared Journal Entries

Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Week 10
Week 11
Week 12
Week 13
Week 14
Week 15