Difference between revisions of "Lenaolufson Week 2"

Revision as of 03:38, 15 September 2015

Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’

There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
- In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
- The genetic code is read in the 5’ to 3’ direction.
- Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.

Note: For the full list of codons, see this RNA Codon Table. The stop codon is indicated by "x".

@@ Line 13: / Line 13: @@
 * Note: For the full list of codons, see this [https://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table RNA Codon Table]. The stop codon is indicated by "x".
+===Translated Reading Frames===
+* +1 N-ter-RMLIPCSAYNPAASSAGGIL-C-ter
+**this is an open reading frame
+* +2 N-ter-VC-C-ter
+* +3 N-ter-YANTMFRV-C-ter
+* -1 stop codon
+* -2 N-ter-KMPPAELAAGLYAEHGISI-C-ter
+** this is an open reading frame
+* -3 N-ter-KCRQRNWRLGYTRNMVLAY-C-ter
+** this is an open reading frame