Difference between revisions of "Kevin Wyllie Week 2"
From LMU BioDB 2015
(I went ahead and added the "Nter" and "Cter" notation just for explicit clarity.) |
(Even though they weren't asked for, I went ahead and added the mRNA sequences for each frame so that I could more easily double-check my answers.) |
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| Line 2: | Line 2: | ||
The given DNA sequence is written in the top strand, while its complementary strand is shown below it. | The given DNA sequence is written in the top strand, while its complementary strand is shown below it. | ||
| − | |||
5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta- 3' | 5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta- 3' | ||
| − | 3'- | + | 3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat- 5' |
Translated reading frames: | Translated reading frames: | ||
| − | * '''+1''' Nter-R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L-Cter '''(This is an open reading frame.)''' | + | * '''+1''' "cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gct ggc ggc auu uua" |
| − | * '''+2''' Nter-V-C-Cter | + | ** Nter-R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L-Cter '''(This is an open reading frame.)''' |
| − | * '''+3''' Nter-Y-A-N-T-M-F-R-V-Cter | + | * '''+2''' "gua ugc uaa uac cat guu ccg cgu ata acc cag ccg cca guu ccg ctg gcg gca uuu" |
| − | * '''-1''' The first codon in this reading frame is a STOP codon, so there is no polypeptide. | + | **Nter-V-C-Cter |
| − | * '''-2''' Nter-K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I-Cter '''(This is an open reading frame.)''' | + | * '''+3''' "uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cat uuu" |
| − | * '''-3''' Nter-K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y-Cter '''(This is an open reading frame.)''' | + | ** Nter-Y-A-N-T-M-F-R-V-Cter |
| + | * '''-1''' "uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga aca ugg uau uag cau acg" | ||
| + | **The first codon in this reading frame is a STOP codon, so there is no polypeptide. | ||
| + | * '''-2''' "aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cau ggu auu agc aua" | ||
| + | ** Nter-K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I-Cter '''(This is an open reading frame.)''' | ||
| + | * '''-3''' "aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac aug gua uua gca uac" | ||
| + | ** Nter-K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y-Cter '''(This is an open reading frame.)''' | ||
Revision as of 05:11, 15 September 2015
Journal Week 2
The given DNA sequence is written in the top strand, while its complementary strand is shown below it.
5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta- 3' 3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat- 5'
Translated reading frames:
- +1 "cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gct ggc ggc auu uua"
- Nter-R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L-Cter (This is an open reading frame.)
- +2 "gua ugc uaa uac cat guu ccg cgu ata acc cag ccg cca guu ccg ctg gcg gca uuu"
- Nter-V-C-Cter
- +3 "uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cat uuu"
- Nter-Y-A-N-T-M-F-R-V-Cter
- -1 "uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga aca ugg uau uag cau acg"
- The first codon in this reading frame is a STOP codon, so there is no polypeptide.
- -2 "aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cau ggu auu agc aua"
- Nter-K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I-Cter (This is an open reading frame.)
- -3 "aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac aug gua uua gca uac"
- Nter-K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y-Cter (This is an open reading frame.)
