Difference between revisions of "Jwoodlee Week 2"

Latest revision as of 19:20, 19 September 2015

5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3'
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'

• There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence.

mRNA sequence(top): 5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3'
mRNA sequence(bottom): 3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5'

+1 5'-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3'

Amino Acids:Nter-R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L-Cter

+2 5'-c gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua-3'

Amino Acids:Nter-V-C-Cter

+3 5'-cg uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a-3'

Amino Acids:Nter-Y-A-N-T-M-F-R-V-Cter

-1 5'-uaa aau gcc gcc agc gga acu ggc ggc ugg guu aua cgc gga aca ugg uau uag cau acg-3'

Amino Acids: None, the first codon is a stop codon

-2 5'-u aaa aug ccg cca gcg gaa cug gcg gcu ggg uua uac gcg gaa cau ggu auu agc aua cg-3'

Amino Acids:Nter-K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I-Cter

-3 5'-ua aaa ugc cgc cag cgg aac ugg cgg cug ggu uau acg cgg aac aug gua uua gca uac g-3'

Amino Acids:Nter-K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y-Cter

• Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
  • +1, -2, and -3 are open reading frames.

Electronic Lab Notebook

Week 2 Assignment:

1. Wrote out complementary DNA strand.
2. Transcribed the DNA strand into two different mRNA strands.
3. Broke up the mRNA strands in to 6 different reading frames 3 different frames per mRNA strand.
4. Translated mRNA codons to amino acids using the mRNA codon translation chart.
5. Wrote down the open reading frames at the end of the question.

BIOL 367, Fall 2015, User Page, Team Page