Difference between revisions of "Emilysimso Week 11"

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(worked on experimental design, vocab)
 
(Experimental Design: dye swaps)
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**Three samples per treatment
 
**Three samples per treatment
 
*Number of chips: 12 (3 samples x 4 treatments = 12)
 
*Number of chips: 12 (3 samples x 4 treatments = 12)
 +
*Dye swaps - unable to find evidence of this in the paper
  
 
==Vocab==
 
==Vocab==

Revision as of 00:05, 12 November 2015

Experimental Design

  • Control: wild-type S. oneidensis MR-1
  • Treatment: used 2,2'-dipyridyl diluted to 80, 160, 240, and 320 micro-molar concentrations
  • Biological treatments - all of the studies were done in triplicates
    • Three samples per treatment
  • Number of chips: 12 (3 samples x 4 treatments = 12)
  • Dye swaps - unable to find evidence of this in the paper

Vocab

  1. Putative: entity or concept that is based on what is generally accepted or inferred even without direct proof
  2. Alcaligin: dihydroxamate siderophore, involved with important pathogens
  3. Enzyme prosthetic group: required for enzyme function
  4. Iron chelating: detoxify metal ions and prevent poisoning, removing excess iron
  5. Siderophores: ferric ion specific chelating agent, make iron available to cell
  6. Heme: nonprotein, insoluble, iron protoporphyrin constituent of hemoglobin; iron compound, pigment portion or protein-free part of hemoglobin
  7. Orthology: homologous genes related by speciation
  8. 2-2'-dipyridyl: chelator of iron