Difference between revisions of "Emilysimso Week 11"
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Emilysimso (Talk | contribs) (→Experimental Design: dye swaps) |
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*Control: wild-type ''S. oneidensis'' MR-1 | *Control: wild-type ''S. oneidensis'' MR-1 | ||
*Treatment: used 2,2'-dipyridyl diluted to 80, 160, 240, and 320 micro-molar concentrations | *Treatment: used 2,2'-dipyridyl diluted to 80, 160, 240, and 320 micro-molar concentrations | ||
| + | **This is to reduce the iron uptake abilities of the samples | ||
*Biological treatments - all of the studies were done in triplicates | *Biological treatments - all of the studies were done in triplicates | ||
**Three samples per treatment | **Three samples per treatment | ||
Revision as of 00:24, 12 November 2015
Experimental Design
- Control: wild-type S. oneidensis MR-1
- Treatment: used 2,2'-dipyridyl diluted to 80, 160, 240, and 320 micro-molar concentrations
- This is to reduce the iron uptake abilities of the samples
- Biological treatments - all of the studies were done in triplicates
- Three samples per treatment
- Number of chips: 12 (3 samples x 4 treatments = 12)
- Dye swaps - unable to find evidence of this in the paper
Vocab
- Putative: entity or concept that is based on what is generally accepted or inferred even without direct proof
- [Citation]
- Alcaligin: dihydroxamate siderophore, involved with important pathogens
- [Citation]
- Enzyme prosthetic group: required for enzyme function
- [Citation]
- Iron chelating: detoxify metal ions and prevent poisoning, removing excess iron
- [Citation]
- Siderophores: ferric ion specific chelating agent, make iron available to cell
- [Citation]
- Heme: nonprotein, insoluble, iron protoporphyrin constituent of hemoglobin; iron compound, pigment portion or protein-free part of hemoglobin
- [Citation]
- Orthology: homologous genes related by speciation
- [Citation]
- 2-2'-dipyridyl: chelator of iron
- [Citation]
