*What were the methods used in the study?  

*What were the methods used in the study?  

**First, the researchers amplified a strand of wild-type S. oneidensis MR-1 using PCR. They confirmed that they had the desired sequence using DNA sequencing. (14)

**First, the researchers amplified a strand of wild-type S. oneidensis MR-1 using PCR. They confirmed that they had the desired sequence using DNA sequencing. (14)

**To test the uptake of iron in aerobic versus anaerobic conditions, the MR-1 was first grown to mid-log phase and diluted. They then created diluted solutions of 2,2’-dipyridyl in 80 M, 160 M, 240 M, and 320 M concentrations. This was done in triplicates for each condition. Next, ferrous sulfate solution was added to replete the iron. They used a Luria-Bertani medium at 30 C for the aerobic culturing and supplemented this medium with 10mM lactate and an electron acceptor for the anaerobic culturing. (14)

**To test the uptake of iron in aerobic versus anaerobic conditions, the MR-1 was first grown to mid-log phase and diluted. They then created diluted solutions of 2,2’-dipyridyl in 80 micro-M, 160 micro-M, 240 micro-M, and 320 micro-M concentrations. This was done in triplicates for each condition. Next, ferrous sulfate solution was added to replete the iron. They used a Luria-Bertani medium at 30 C for the aerobic culturing and supplemented this medium with 10mM lactate and an electron acceptor for the anaerobic culturing. (14)

**To test the iron reduction rate, cells were grown anaerobically to mid-log phase in the LB medium along with 10mM fumarate and 10mM lactate. They were transferred to a solution of 5mL LB medium, 10mM lactate, and 10mM Fe(III) dioxide. They then performed the ferrozine assay. (14).

**To test the iron reduction rate, cells were grown anaerobically to mid-log phase in the LB medium along with 10mM fumarate and 10mM lactate. They were transferred to a solution of 5mL LB medium, 10mM lactate, and 10mM Fe(III) dioxide. They then performed the ferrozine assay. (14).

**For the microarray portion of the experiment, four biological replicates were grown in the LB medium. 2,2’-dipyridyl was added at concentration of 160 M, followed by ferrous sulfate after 60 minutes. They then centrifuged to allow for RNA extraction.

**For the microarray portion of the experiment, four biological replicates were grown in the LB medium. 2,2’-dipyridyl was added at concentration of 160 micro-M, followed by ferrous sulfate after 60 minutes. They then centrifuged to allow for RNA extraction.

*Briefly state the result shown in each of the figures and tables.  

*Briefly state the result shown in each of the figures and tables.