Lenaolufson Week 2

Individual Journal Assignment

The Genetic Code

• Write out the complementary strand of DNA below the strand shown and be sure to label the 5’ and 3’ ends of the complementary strand.

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’

• There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence, keeping in mind the following rules.
  • In RNA, the T becomes a U, so everywhere you see a T in the sequence, read it as a U.
  • The genetic code is read in the 5’ to 3’ direction.
  • Use the single-letter abbreviations for the amino acids because that is what is commonly used by computer programs.

• Note: For the full list of codons, see this RNA Codon Table. The stop codon is indicated by "x".

Translated Reading Frames

• +1 N-ter-RMLIPCSAYNPAASSAGGIL-C-ter
  • this is an open reading frame
• +2 N-ter-VC-C-ter
• +3 N-ter-YANTMFRV-C-ter
• -1 stop codon
• -2 N-ter-KMPPAELAAGLYAEHGISI-C-ter
  • this is an open reading frame
• -3 N-ter-KCRQRNWRLGYTRNMVLAY-C-ter
  • this is an open reading frame