Emilysimso Week 11

Experimental Design

• Control: wild-type S. oneidensis MR-1
• Treatment: used 2,2'-dipyridyl diluted to 80, 160, 240, and 320 micro-molar concentrations
  • This is to reduce the iron uptake abilities of the samples
• Biological treatments - all of the studies were done in triplicates
  • Three samples per treatment
• Number of chips: 12 (3 samples x 4 treatments = 12)
• Dye swaps - unable to find evidence of this in the paper

Vocab

1. Putative: entity or concept that is based on what is generally accepted or inferred even without direct proof
   • [Citation]
2. Alcaligin: dihydroxamate siderophore, involved with important pathogens
   • [Citation]
3. Enzyme prosthetic group: required for enzyme function
   • [Citation]
4. Iron chelating: detoxify metal ions and prevent poisoning, removing excess iron
   • [Citation]
5. Siderophores: ferric ion specific chelating agent, make iron available to cell
   • [Citation]
6. Heme: nonprotein, insoluble, iron protoporphyrin constituent of hemoglobin; iron compound, pigment portion or protein-free part of hemoglobin
   • [Citation]
7. Orthology: homologous genes related by speciation
   • [Citation]
8. 2-2'-dipyridyl: chelator of iron
   • [Citation]

Flow Chart

• Initial flow chart

Weekly Assignment Information

User: Emilysimso

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Group Project

Heavy Metal HaterZ