Vpachec3 Week 3

The Genetic Code, by Computer

  cat prokaryote.txt | see "y/atcg/tagc/"

Reading Frames

+1

cat prokaryote.txt | sed "s/..$//g" | sed "y/t/u/" | sed "s/.../& /g" | sed -f genetic-code.sed 

 S T I F Q - V R W P K K T I L N L K R C L I P C S A Y N P A A S S A G G I L

+2

cat prokaryote.txt | sed "s/^.//g" | sed "s/.$//g" | sed "y/t/u" | sed "s/.../& /g" | sed -f genetic-code.sed

L L Y F N R Y D G Q R R Q Y - T - N V A - Y H V P R I T Q  P P V P L A  A  F -

+3

cat prokaryote.txt | sed "s/^..//g" | sed "y/t/u/" | sed "s/.../& /g" | sed -f genetic-code.sed

Y Y I S I G T M A K E D N I E L E T L P N  T M F R V - P S R Q F R W R H F N

-1

cat prokaryote.txt | sed "y/tagc/aucg/" | rev | sed "s/.../& /g" | sed "s/..$//g" | sed -f genetic-code.sed

V K M P P A E L A A G L Y A E H G I R Q R F K F N I V F F G H R T Y - N I V

-2

 cat prokaryote.txt | sed "y/tagc/aucg" | rev | sed "s/^..//g" | sed "s/.../& /g" | sed -f genetic-code.sed

L K C R Q R N W R L G Y T  R N M V L G N V S S S I L S S L A I V P I E I - -

-3

cat prokaryote.txt | sed "y/tagc/aucg/" | rev | sed "s/^..//g" | sed "s/.../& /g" | sed -f genetic-code.sed

N A A S G T G G W V I R G T W Y - A T F Q V Q Y C L L W P S Y L L K Y S R

XMLPipeDB Match Practice

What Match command tallies the occurrences of the pattern GO:000[567] in the 493.P_falciparum.xml file? How many unique matches are there?

There were 3 unique matches.

How many times does each unique match appear?

go:0007: 113
go:0006: 1100
go:0005: 1371

Try to find one such occurrence “in situ” within that file. Look at the neighboring content around that occurrence. Describe how you did this.

grep “GO:000[567]” 493.P_falciparum.xml

Based on where you find this occurrence, what kind of information does this pattern represent?

What Match command tallies the occurrences of the pattern \"Yu.*\" in the 493.P_falciparum.xml file? How many unique matches are there? How many times does each unique match appear? What information do you think this pattern represents?

Use Match to count the occurrences of the pattern ATG in the hs_ref_GRCh37_chr19.fa file (this may take a while). Then, use grep and wc to do the same thing. What answer does Match give you? What answer does grep + wc give you? Explain why the counts are different. (Hint: Make sure you understand what exactly is being counted by each approach.)

Electronic Lab Book

1. Go to the magnifying glass symbol at the top of the computer screen and type in ' Terminal'
2. Click on Terminal and type in: ssh my dot cs dot lmu dot edu and click 'Enter'
3. Type in password and press enter
4. ( I personally took a while to figure this out) Then type: cd ~dondi

Links

Vpachec3 User Page