Troque Week 8

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Normalize the log ratios for the set of slides in the experiment

To scale and center the data (between chip normalization) perform the following operations:

  • Insert a new Worksheet into your Excel file, and name it "scaled_centered".
  • Go back to the "compiled_raw_data" worksheet, Select All and Copy. Go to your new "scaled_centered" worksheet, click on the upper, left-hand cell (cell A1) and Paste.
  • Insert two rows in between the top row of headers and the first data row.
  • In cell A2, type "Average" and in cell A3, type "StdDev".
  • You will now compute the Average log ratio for each chip (each column of data). In cell B2, type the following equation:
=AVERAGE(B4:B5224)

Note: We tried to do a keyboard shortcut using CTRL + Shift + Down buttons, but row 363 has a missing data so we had to manually type in "B5224" for the end of the data.

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