Week 11 E-notes Eyanosch

Week 11 Electronic Notes

1. 10 Biological terms
   • shigellosis - Shigella infection (shigellosis) is an intestinal disease caused by a family of bacteria known as shigella. The main sign of shigella infection is diarrhea, which often is bloody. (http://www.mayoclinic.org/diseases-conditions/shigella/basics/definition/con-20028418)
   • rifampin
   • rifaximin
   • pouchitis
   • putative
   • MIC
   • type III secretion system
   • transient
   • attenuation
   • nascent

1. Article Outline
   • What is the importance or significance of this work (i.e., your species)?

The importance of this work is to identify which genes are induced or repressed depending on the drug tested. These bactrium are responsible for shigellosis, a bacterial infection of the intestine that results in bloody diarrhea and fever. Two antibiotic compounds were studied, rifampin (RP) and rifaximin (RX), both inhibiting RNA synthesis which inhibits growth and reproduction processes of the bacteria. Due to recently increasing resistance to antibiotics in shigella strains it is imperative to see which genes are being inhibited or induced with the current market antibiotics. The expression profiles of micro arrays will provide insight into the interaction between the drugs and bacteria. By inhibiting RNA synthesis, no new proteins will be produced, any proteins still present have yet to degrade.

1. • What were the methods used in the study?

1. • Briefly state the result shown in each of the figures and tables.

1. • How do the results of this study compare to the results of previous studies (See Discussion).

1. • For the microarray paper (GenMAPP Users only), include the following:

1. • 1. Describe the experimental design of the microarray data, including treatments, number of replicates (biological and/or technical), dye swaps.

1. • 1. Determine the sample and data relationships, i.e., which files in the data correspond to which samples in the experimental design.

1. • 1. Construct a flow chart that illustrates the above.

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