Vpachec3 Week 12
From LMU BioDB 2015
Thursday, November 19
- Met with Dr. Dahlquist before class to talk about the paper
- Took note of the microarray data versus the qPCR results. Some results were off and will keep that in mind during our analysis
- GenMapp users with Dr.Dahlquist during class to go over progress
- Rough summary of experiment design:
- 5 untreated biofilms vs. genomic DNA
- 3 tobramycin-treated biofilm vs. genomic DNA
- Noticed that our sdrf file was imputed wrong
- Need to ask authors what they did with the dye swaps
- Started computing master raw data
- For each text file(8), we are copying rows M(gene name) and R (log ratio) into one data sheet
- Decided to make names side by side for our own organization style
- Made three sheets in the file:allgenename_logratio, genename_logratio, clean_genename_logratio
- For each text file(8), we are copying rows M(gene name) and R (log ratio) into one data sheet
