Emilysimso Week 15

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Revision as of 23:35, 12 December 2015 by Emilysimso (Talk | contribs)

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Procedure

  • Calculated averages from split data for each time point and replicate
    • Total of 5409 genes
  • Calculated biological averages of each time point
  • Calculated AverageLogRatio comparing C5, C20, and C60 to C0 and F5, F20, and F60 to C60
    • Subtracted not divided due to log space
  • Performed TTest on the above relationships to get the Pvalue
  • Performed Bonferroni
  • Performed Benjamini & Hochberg
  • Created new sheet forGenMAPP
    • Did adjustments to prepare file for GenMAPP
    • Saved as .txt and uploaded to wiki

GenMAPP

  • Increase criterea = >0.25 AND B-H <0.05
  • Decrease criterea <-0.25 AND B-H <0.05
  • 1441 errors in the raw data
  • Red is increased, green is decreased
  • Uploaded C5-C0, C20-C0, C60-C0, F5-C60, F20-C60, and F60-C60 into GenMAPP

MAPPFinder

  • Criteria: Zscore >2, Permute P<0.05, Number Changed > or equal to 4 < or equal to 100, Percent Changed > or equal to 50%

Files


Weekly Assignment Information

User: Emilysimso

Assignments

Individual Journal Entries

Class Journal Entries

Group Project

Heavy Metal HaterZ