Jwoodlee Week 2

5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3'
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'

• There are six possible reading frames in double-stranded DNA. Using the genetic code, translate all possible reading frames of this DNA sequence.

mRNA sequence(top): 5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3'
mRNA sequence(bottom): 3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'

+1 5'-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3'
Amino Acids:
+2 5'-c gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua-3'
Amino Acids:
+3 5'-cg uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a-3'
Amino Acids:

-1 5'-taa aat gcc gcc agc gga act ggc ggc tgg gtt ata cgc gga aca tgg tat tag cat acg-3'
-2 5'-t aaa atg ccg cca gcg gaa ctg gcg gct ggg tta tac gcg gaa cat ggt att agc ata cg-3'
-3 5'-ta aaa tgc cgc cag cgg aac tgg cgg ctg ggt tat acg cgg aac atg gta tta gca tac g-3'

• Which of the reading frames (if any) of the reading frames you translated is an open reading frame, i.e., does not contain a stop codon?
  • By convention, the top strand frames are called +1, +2, +3, reading 5' to 3' and the bottom strand frames are called -1, -2, -3, reading 5' to 3'.

BIOL 367, Fall 2015, User Page, Team Page

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