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		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/api.php?action=feedcontributions&amp;feedformat=atom&amp;user=Kwrigh35</id>
		<title>LMU BioDB 2017 - User contributions [en]</title>
		<link rel="self" type="application/atom+xml" href="https://xmlpipedb.lmucs.io/biodb/fall2017/api.php?action=feedcontributions&amp;feedformat=atom&amp;user=Kwrigh35"/>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php/Special:Contributions/Kwrigh35"/>
		<updated>2026-06-27T15:30:06Z</updated>
		<subtitle>User contributions</subtitle>
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	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5891</id>
		<title>Lights, Camera, InterACTION! Deliverables</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5891"/>
				<updated>2017-12-16T00:26:46Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Individual Statements */ indicated that my personal statement is expected via email&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;===Group Report and Presentation===&lt;br /&gt;
Group Report: [[Media:LCI_Group_Report.pdf]]&lt;br /&gt;
&lt;br /&gt;
Presentation: [[Media:LCI Presentation.pdf]]&lt;br /&gt;
&lt;br /&gt;
===Individual Statements===&lt;br /&gt;
[[Emma&amp;#039;s Individual Statement]]&lt;br /&gt;
&lt;br /&gt;
Blair&amp;#039;s Individual Statement: &amp;#039;&amp;#039;&amp;#039;emailed&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
Katie&amp;#039;s Individual Statement: &amp;#039;&amp;#039;&amp;#039;emailed&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
===Code and README===&lt;br /&gt;
[https://github.com/bhamilton18/GRNsight GRNsight GitHub Fork]&lt;br /&gt;
&lt;br /&gt;
[https://github.com/bhamilton18/GRNsight/blob/master/web-client/public/gene/integrationREADME.md README.md File]&lt;br /&gt;
&lt;br /&gt;
===ANOVA and stem results, gene list and GO list files===&lt;br /&gt;
# Excel workbook: [[Media:Wt_Microarraydata_ET.zip]]&lt;br /&gt;
# File used to run stem, gene list tables, GO list tables: [[Media:Week10_filesforstem_wildtype_emmat.zip‎]]&lt;br /&gt;
# Powerpoint presentation with stem images (also linked below under GRNmap workbooks): [[Media:Wt_profileimage_presentation_emmat.pptx]]&lt;br /&gt;
&lt;br /&gt;
===YEASTRACT results===&lt;br /&gt;
# YEASTRACT rank by TF results: [[Media:Yeastract_profile45results_preserved_emmat.xlsx]]&lt;br /&gt;
&lt;br /&gt;
===GRNmap input and output workbooks===&lt;br /&gt;
# Final regulation matrix: [[Media:Final_regulationmatrix_trimmed.xlsx]]&lt;br /&gt;
# Input workbook: [[Media:17-genes_39-edges_teamINT_Sigmoid_estimation.xlsx]]&lt;br /&gt;
# Output workbook after Matlab: [[Media:Matlab_output_17-genes_39-edges_teamINT_Sigmoid_estimation_output_copy.xlsx]]&lt;br /&gt;
# Weighted and unweighted GRNmap images:  [[Media:GRNmap_images_emma_t.zip]]&lt;br /&gt;
# Individual gene .jpg&amp;#039;s from matlab output: [[Media:Emmat_gene_jpg_matlab.zip]]&lt;br /&gt;
&lt;br /&gt;
===Electronic notebook corresponding to microarray results===&lt;br /&gt;
# [[Emmatyrnauer Week 8]]&lt;br /&gt;
# [[Emmatyrnauer Week 10]]&lt;br /&gt;
# [[Emmatyrnauer Week 11]]&lt;br /&gt;
# [[Emmatyrnauer Week 12]]&lt;br /&gt;
# [[Emmatyrnauer Week 14]]&lt;br /&gt;
# [[Emmatyrnauer Week 15]]&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=File:LCI_Group_Report.pdf&amp;diff=5888</id>
		<title>File:LCI Group Report.pdf</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=File:LCI_Group_Report.pdf&amp;diff=5888"/>
				<updated>2017-12-16T00:19:30Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: Kwrigh35 uploaded a new version of File:LCI Group Report.pdf&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;up to date group report name and current draft&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=File:LCI_Presentation.pdf&amp;diff=5676</id>
		<title>File:LCI Presentation.pdf</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=File:LCI_Presentation.pdf&amp;diff=5676"/>
				<updated>2017-12-12T08:17:37Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: Kwrigh35 uploaded a new version of File:LCI Presentation.pdf&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=5675</id>
		<title>Lights, Camera, InterACTION!</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=5675"/>
				<updated>2017-12-12T08:03:40Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* File Roundup */ added link to final presentation&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:PugNotDrugsLogo.png|400px|thumb|right|Pugs Not Drugs Logo]]&lt;br /&gt;
&lt;br /&gt;
==Team Members==&lt;br /&gt;
*[[user:bhamilton18|Blair Hamilton]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:emmatyrnauer|Emma Tyrnauer]] &amp;#039;&amp;#039;&amp;#039;-Data Analyst&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:kwrigh35|Katie Wright]] &amp;#039;&amp;#039;&amp;#039;-Project Manager/Quality Assurance&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:zvanysse|Zach Van Ysseldyke]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
==File Roundup==&lt;br /&gt;
*Final Presentation: [[media:LCI Presentation.pdf]]&lt;br /&gt;
*Week 11 presentation by Blair and Zach: [[media:The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf|The Secret Startup that Saved the Worst Website in America]]&lt;br /&gt;
*Week 12 presentation by Emma and Katie: [[media:Genome-wide expression analysis of yeast response during exposure to 4 degrees C.pdf|Genome-wide expression analysis of yeast response during exposure to 4 degrees C]]&lt;br /&gt;
*Microarray data excel spreadsheet (corrected) : [[Media:Wt_Microarraydata_ET.zip]]&lt;br /&gt;
[[Lights, Camera, InterACTION! Deliverables]]&lt;br /&gt;
&lt;br /&gt;
==Team Schedule== &amp;lt;!--Borrowed Format from JASPAR group--&amp;gt;&lt;br /&gt;
{| class =&amp;quot;wikitable&amp;quot;&lt;br /&gt;
|+November &amp;amp; December&lt;br /&gt;
|-&lt;br /&gt;
|Sunday&lt;br /&gt;
|Monday&lt;br /&gt;
|Tuesday&lt;br /&gt;
|Wednesday&lt;br /&gt;
|Thursday&lt;br /&gt;
|Friday&lt;br /&gt;
|Saturday&lt;br /&gt;
|-&lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt; C: PPT Presentation&amp;lt;br&amp;gt;PM/DA: Journal Articles&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;17&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;18&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;19&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;20&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;21&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;C: Complete setup steps (Milestones 0-4)&amp;lt;br&amp;gt;PM/DA: Journal Club Presentations&amp;lt;br&amp;gt;DA: Week 8 and Week 10 edits&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;22&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;23&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;24&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;25&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;26&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;27&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;28&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;C: First Integration projected completion&amp;lt;/s&amp;gt;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;PM: Check in with team &amp;lt;br&amp;gt;DA: &amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;29&amp;#039;&amp;#039;&amp;#039; &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;30&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;C: Check in with other groups about progress&amp;lt;/s&amp;gt;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;PM: Check in with team &amp;lt;br&amp;gt;DA:&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;C: Check in with groups, and further integrate as needed.&amp;lt;/s&amp;gt;&amp;lt;br&amp;gt;PM: Check in with team &amp;lt;br&amp;gt;&amp;lt;s&amp;gt;DA: complete week 10 data analysis&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;C: Check in with other groups about progress&amp;lt;/s&amp;gt;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;PM: Check in with team &amp;lt;br&amp;gt;DA: &amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;Final group Presentation &amp;#039;&amp;#039;2:00-4:00PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;All deliverables due &amp;#039;&amp;#039;4:30PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
==Executive Summaries==&lt;br /&gt;
===Week 11===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
For this week, I worked with [[user:emmatyrnauer|Emma]] to find papers to use as reference in our final research paper.  Emma and I both found papers that are related to yeast cold shock reactions and most of them use microarray data.  I am excited to read these articles more in depth as we develop our research project and paper.  Further information can be found on my [[kwrigh35 Week 11|Week 11]] journal page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039; Using the three different sources was really helpful because I had never used &amp;quot;Web of Science&amp;quot; before.  It was good to learn about how different these results can be, especially with tweaking the search terms a little bit.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I had poor time management this week.  I waited until Monday to do the majority of the work and definitely should not have done so.  The rest of the project went well, however.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I will plan to meet up with Emma early on in the week so we can work together on our presentation.  This way I will be held accountable to get my work done earlier.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week Katie and I worked on the annotated bibliography (below). We chose papers that provided more information on yeast gene regulation in response to cold shock. Pub Med, Google Scholar, and Web of Science were utilized to perform basic searches using key words as well as advanced searches which helped to narrow the results. More detailed information can be found on my individual journal entry page: [[Emmatyrnauer Week 11]]. Meanwhile, Blair and Zach worked on coder journal club presentation. Construction of the group page was completed during class.&lt;br /&gt;
# Overall, this week went pretty smoothly; Katie and I communicated over text to determine which papers we were going to write the bibliographies for and were available to each other for any questions we had.&lt;br /&gt;
# There wasn&amp;#039;t really anything that didn&amp;#039;t work.&lt;br /&gt;
# Since we will be working this next week on our journal club presentation, it might be beneficial to meet in person.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:26, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
This week Zach and I created the Powerpoint presentation about &amp;#039;&amp;#039;The secret startup that saved the worst website in America&amp;#039;&amp;#039; article. We created individual outlines of the article on our respective pages, mine is as follows: [[Bhamilton18 Week 11]]. Our presentation is linked here: [[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]]&lt;br /&gt;
#Overall, our communication and diving up of tasks went well and smoothly. Meanwhile, Katie and Emma worked on the annotate bibliography. While I primarily worked with Zach our communication amongst group members went well and we hope to continue to do so for the future.&lt;br /&gt;
#Something that didn&amp;#039;t work as well was our time management. Next time we hope to have more class time and start working earlier on our portion of the assignment. &lt;br /&gt;
#For the future, we will continue to communicate in person and through messaging and will work on time management by starting earlier.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 23:35, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
[[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]] &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 11]]&amp;lt;br&amp;gt;&lt;br /&gt;
This week, Blair and I created a Powerpoint about the &amp;quot;Secret Startup that saved the worst website in America.&amp;quot; We read through the article and picked out the relevant themes and main messages to put on the PowerPoint. We wrote these themes and summaries out on our individual Journals. Then, we collaborated and made our PowerPoint. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; Blair and I&amp;#039;s communication was very strong and we knew exactly what was going on with one another. We knew when we were going to meet up and what was expected for each of us.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Blair and I missed the mark on our time management. We did not expect this assignment to take this long. Next time, we will make sure to start on assignments earlier.&lt;br /&gt;
#Like I said above, we will plan out exact times earlier on in the week that we can meet up&lt;br /&gt;
[[User:Zvanysse|Zvanysse]] ([[User talk:Zvanysse|talk]]) 23:44, 13 November 2017 (PST) &amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
===Week 12===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
Emma and I worked on our presentation on &amp;quot;Genome-wide expression analysis of yeast response during exposure to 4 degrees C,&amp;quot; details of which can be found on my [[kwrigh35 Week 12|Week 12]] page.  The presentation can be found in the &amp;quot;file roundup&amp;quot; section of this page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039;Emma and I met up outside of class to work on the assignment, which was really helpful.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I underestimated to the time it would take to find 10 terms that I didn&amp;#039;t know before reading the paper.  It was hard to find 10, so I resorted to picking terms that I knew but wanted to know more about.  It was also difficult to find definitions for some of these words, because some of them weren&amp;#039;t in biological dictionaries.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I should start work on the assignment before the weekend begins.  That way, I don&amp;#039;t have to spend the majority of my Monday afternoon/evening on the project.  It is better to be able to come back to a project several times, and each time have &amp;quot;fresh&amp;quot; eyes.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week I made all the corrections listed on the talk page of my user page for both [[Emmatyrnauer Week 8]] and [[Emmatyrnauer Week 10]]. This included corrections to the excel files, stem analysis, electronic notebooks, analysis of log fold changes and p values, and the acknowledgements section. Following the completion of fixing the errors in the excel file, I emailed Dr. Dahlquist to ascertain that I completed the analysis correctly. For the individual assignment this week, Katie and I met up to work on our journal club presentation of the paper that we were assigned (can be accessed on my individual journal page, [[Emmatyrnauer Week 12]]. We divided up what portions we would be responsible for and have planned to meet up before class tomorrow to practice. The paper we were assigned is: Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., ... &amp;amp; Fujita, K. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. The powerpoint presentation and updated excel file can be accessed above under &amp;quot;File Roundup.&amp;quot;&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; It was very helpful to meet up in person with Katie to begin working on the powerpoint before separating everything out.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; This week I had so much to do including fixing both week 8 and week 10 individual pages and redoing stem because of the mistake I made in excel. I could have asked for an extension on the excel files to allow for more time to work on the presentation. I could have also been in better communication with Blair and Zach this week.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I will communicate more with the professors. Sitting down together with my group will also help to get us all on the same page for the future weeks.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:03, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
As mentioned on my electronic notebook, [[Bhamilton18 Week 12]], this week&amp;#039;s assignment Zachary Van Ysseldyk and I worked on the Coder Milestones 0-4. Zach and I will be working on the integration of the website bringing together the other groups work to function on the GRNsight website. Katie and Emma worked on their respective journal club presentation, but we communicated to check base on how everyone is doing in the progression of the project. Zach and I met in person to collaborate on formatting of the team page, individual journal pages and project progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; Zach and I worked on better time management as well as expectations for the assignment. We were able to communicate without any hiccups, and meeting up in person was ideal for clarifying issues.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; We were unsure of our progress in the project as our understanding of milestone 4 meant we needed to wait this week on others. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Checking in with our project teams/coders will help us understand how they are doing, as well as how our own progression is lining up.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 19:15, 20 November 2017 (PST)&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
This week, Blair and I worked on the coder milestones. We will work on integrating the actual other team&amp;#039;s parts as they start to finish. We also collaborated with Emma and Katie to make sure that we were on the same page. &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 12]]&amp;lt;br&amp;gt;&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; we were better about our time management as well as we communicated well. We met up together periodically to make sure that we were on the same page.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; We were a little confused on how to go about the project for our milestone considering that it was more of a waiting game this week rather than actual integration. So, we didn&amp;#039;t really know how to quantify our progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What we will do to fix it:&amp;#039;&amp;#039;&amp;#039; We maybe should have collaborated with the other teams a little bit more to be able and gauge how their projects are coming along.&lt;br /&gt;
&lt;br /&gt;
===Week 14===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week I worked on completing the week 10 analysis and visualized a gene regulatory network for the wild type strain with GRNsight. All of the steps I took and files used can be found on my individual page: [[Emmatyrnauer Week 14]].&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; It was nice that I had already completed the week 8 and week 10 corrections so that I could just focus on completing the data analysis. I went in to talk to Dr. Dahlquist with issues that I was having during analysis of the microarray data. This allowed for faster and accurate completion of the analysis. I was also able to contact the data analysis guild with any questions that I had. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Everything went pretty smoothly this week; it was very helpful having work time in class to complete my last milestone.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What we will do to fix it:&amp;#039;&amp;#039;&amp;#039; For next week, I will continue to work with the data analyst guild to complete final individual tasks for the project. I will also be collaborating more with my group members to pull together the final deliverables, paper, and presentation.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 19:31, 4 December 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
This week Zach and I worked on the first integration of the website and added a right click feature to each gene. We met in person and text messaged each other. As mentioned in my electronic notebook, [[Bhamilton18 Week 14]], we found online resources to add this right click feature as well as collaborate with Dr. Dionisio for syntax problems. This week Emma focused on progressing with data analysis for the gene wild type and then Katie made sure all groups were giving us their deliverables.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; Collaboration with other groups was productive and went smoothly. Having Dr. Dionisio as a resource has been very helpful and quickened the process.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Working in D3 syntax has been difficult to progress in certain deliverables. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What we will do to fix it:&amp;#039;&amp;#039;&amp;#039; In order to fix this problem, Zach and I hope to work closely with Dr. Dionisio finding external resources and fixing small errors. &lt;br /&gt;
&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 21:25, 4 December 2017 (PST)&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
This week Blair and I worked on the first integration by merging through github as well as working on the right click function. This function enabled us to right click and automatically link the gene that was clicked on to that particular gene page. We connected by texting as well as meeting up in person before committing our week&amp;#039;s work to make sure that we were on the same page. We mentioned this on my electronic notebook, [[Zvanysse Week 14]]. We collaborated with Dr. Dionisio to work out some of the D3 technical syntax problems that came about. Emma worked on progressing on the data analysis for the gene wild type and Katie make sure that everyone was on task.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What went well:&amp;#039;&amp;#039;&amp;#039; Our communication and procrastination was overall much better. Collaborating with other teams went smoothly and connecting with Dr. Dionisio for syntax went well as the problem was diagnosed soon after it became about.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What Didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Working with D3 was a little difficult because the syntax ultimately changed for the API. This was unforeseen so it was a little frustrating.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;How will we fix this:&amp;#039;&amp;#039;&amp;#039; When it comes to syntax problems, we will be more in communication with Dr. Dionisio &amp;lt;br&amp;gt;&lt;br /&gt;
[[User:Zvanysse|Zvanysse]] ([[User talk:Zvanysse|talk]]) 21:36, 4 December 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;!----&lt;br /&gt;
===Week 15===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
---&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Bibliography==&lt;br /&gt;
===Paper 1===&lt;br /&gt;
Homma, T., Iwahashi, H., &amp;amp; Komatsu, Y. (2003). Yeast gene expression during growth at low temperature. &amp;#039;&amp;#039;Cryobiology&amp;#039;&amp;#039;, 46(3), 230-237. &lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/12818212&lt;br /&gt;
* PubMed Central:  not available&lt;br /&gt;
* Publisher Full Text (HTML):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=12818212&lt;br /&gt;
* Publisher Full Text (PDF): https://electra.lmu.edu:3555/S0011224003000282/1-s2.0-S0011224003000282-main.pdf?_tid=4c9b7cc0-c775-11e7-af71-00000aab0f26&amp;amp;acdnat=1510469407_3e02bee4266fd6a211ea3f6e27b460cf &lt;br /&gt;
* Copyright: 2003 by Science Direct; article is not Open Access&lt;br /&gt;
* Publisher:  Elsevier Science&lt;br /&gt;
* Availability: online at www.sciencedirect.com&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 2===&lt;br /&gt;
Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., ... &amp;amp; Fujita, K. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. &amp;#039;&amp;#039;Extremophiles&amp;#039;&amp;#039;, 10(2), 117-128.&lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/?term=Genome-wide+expression+analysis+of+yeast+response+during+exposure+to+4+degrees+C&lt;br /&gt;
* PubMed Central: not available&lt;br /&gt;
* Publisher Full Text (HTML): https://electra.lmu.edu:2529/article/10.1007/s00792-005-0480-1&lt;br /&gt;
* Publisher Full Text (PDF):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=16254683&lt;br /&gt;
* Copyright: 2005 by Springer-Verlag; article is not Open Access&lt;br /&gt;
* Publisher:  Springer-Verlag&lt;br /&gt;
* Availability: online&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 3===&lt;br /&gt;
Becerra, M., Lombardia, L. J., González‐Siso, M. I., Rodríguez‐Belmonte, E., Hauser, N. C., &amp;amp; Cerdán, M. E. (2003). Genome‐wide analysis of the yeast transcriptome upon heat and cold shock. Comparative and functional genomics, 4(4), 366-375.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/pdf/CFG-04-366.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML):&amp;#039;&amp;#039;&amp;#039; unavailable &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF):&amp;#039;&amp;#039;&amp;#039; http://downloads.hindawi.com/journals/ijg/2003/175356.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright:&amp;#039;&amp;#039;&amp;#039; Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher:&amp;#039;&amp;#039;&amp;#039; Hindawi Publishing Corporation&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability:&amp;#039;&amp;#039;&amp;#039; Open access&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; no&lt;br /&gt;
&lt;br /&gt;
===Paper 4===&lt;br /&gt;
Sahara, T., Goda, T., &amp;amp; Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract: &amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pubmed/?term=Comprehensive+expression+analysis+of+time-dependent+genetic+responses+in+yeast+cells+to+low+temperature&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central: &amp;#039;&amp;#039;&amp;#039; not available &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.long&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.full.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright: &amp;#039;&amp;#039;&amp;#039; &amp;quot;Other parties are welcome to copy, distribute, transmit and adapt the work — at no cost and without permission — for noncommercial use as long as they attribute the work to the original source using the citation above.&amp;quot; from http://www.jbc.org/site/misc/Copyright_Permission.xhtml&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher: &amp;#039;&amp;#039;&amp;#039; American Society for Biochemistry and Molecular Biology&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability: &amp;#039;&amp;#039;&amp;#039;All articles from this journal are free 1 year after publishing.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; No.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Acknowledgments==&lt;br /&gt;
#Our group collaborates on this page each week filling out our respective team journal assignments and project updates.&lt;br /&gt;
#*WE have a group chat to communicate problems, questions and ideas for our weekly assignments/milestones.&lt;br /&gt;
#We utilized the Calendar outline from the [[JASPAR the Friendly Ghost]] page, but changed the milestone/tasks due.&lt;br /&gt;
&lt;br /&gt;
==References==&lt;br /&gt;
*Meyer, R. (2015, July 09). The Secret Startup That Saved the Worst Website in America. Retrieved November 7, 2017, from https://www.theatlantic.com/technology/archive/2015/07/the-secret-startup- saved-healthcare-gov-the-worst-website-in-america/397784/&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;br /&gt;
&lt;br /&gt;
{{GRNsight Gene Page Project Links}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5674</id>
		<title>Lights, Camera, InterACTION! Deliverables</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5674"/>
				<updated>2017-12-12T08:00:15Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Group Report and Presentation */ added final presentation&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;===Group Report and Presentation===&lt;br /&gt;
Presentation: [[Media:LCI Presentation.pdf]]&lt;br /&gt;
&lt;br /&gt;
===Individual Statements===&lt;br /&gt;
&lt;br /&gt;
===Code and README===&lt;br /&gt;
&lt;br /&gt;
===ANOVA and stem results, gene list and GO list files===&lt;br /&gt;
# Excel workbook: [[Media:Wt_Microarraydata_ET.zip]]&lt;br /&gt;
# File used to run stem, gene list tables, GO list tables: [[Media:Week10_filesforstem_wildtype_emmat.zip‎]]&lt;br /&gt;
# Powerpoint presentation with stem images (also linked below under GRNmap workbooks): [[Media:Wt_profileimage_presentation_emmat.pptx]]&lt;br /&gt;
&lt;br /&gt;
===YEASTRACT results===&lt;br /&gt;
# YEASTRACT rank by TF results: [[Media:Yeastract_profile45results_preserved_emmat.xlsx]]&lt;br /&gt;
&lt;br /&gt;
===GRNmap input and output workbooks===&lt;br /&gt;
# Input: [[Media:RegulationMatrix_yeastract_week10etf.xlsx]]&lt;br /&gt;
# Output (within powerpoint presentation): [[Media:Wt_profileimage_presentation_emmat.pptx]]&lt;br /&gt;
# Final excel workbooks and GRNmap images after Matlab utilized: [[Media:GRNsight_inputoutput_excelandimages.zip]]‎&lt;br /&gt;
# Individual gene .jpg&amp;#039;s from matlab output: [[Media:Emmat_gene_jpg_matlab.zip]]&lt;br /&gt;
&lt;br /&gt;
===Electronic notebook corresponding to microarray results===&lt;br /&gt;
# [[Emmatyrnauer Week 8]]&lt;br /&gt;
# [[Emmatyrnauer Week 10]]&lt;br /&gt;
# [[Emmatyrnauer Week 11]]&lt;br /&gt;
# [[Emmatyrnauer Week 12]]&lt;br /&gt;
# [[Emmatyrnauer Week 14]]&lt;br /&gt;
# [[Emmatyrnauer Week 15]]&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=File:LCI_Presentation.pdf&amp;diff=5673</id>
		<title>File:LCI Presentation.pdf</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=File:LCI_Presentation.pdf&amp;diff=5673"/>
				<updated>2017-12-12T07:59:51Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5480</id>
		<title>Lights, Camera, InterACTION! Deliverables</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5480"/>
				<updated>2017-12-06T00:03:32Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Group Report and Presentation */ added link to draft of presentation&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;===Group Report and Presentation===&lt;br /&gt;
Draft of presentation: [[Media:Draft_LCI_Final_Presentation.pdf]]&lt;br /&gt;
&lt;br /&gt;
===Individual Statements===&lt;br /&gt;
&lt;br /&gt;
===Code and README===&lt;br /&gt;
&lt;br /&gt;
===ANOVA and stem results===&lt;br /&gt;
# Excel workbook: [[Media:Wt_Microarraydata_ET.zip]]&lt;br /&gt;
# File used to run stem, gene list tables, GO list tables: [[Media:Week10_filesforstem_wildtype_emmat.zip‎]]&lt;br /&gt;
&lt;br /&gt;
===Gene List and GO list files===&lt;br /&gt;
&lt;br /&gt;
===YEASTRACT results===&lt;br /&gt;
# YEASTRACT rank by TF results: [[Media:Yeastract_profile45results_preserved_emmat.xlsx]]&lt;br /&gt;
&lt;br /&gt;
===GRNmap input and output workbooks===&lt;br /&gt;
# Input: [[Media:RegulationMatrix_yeastract_week10etf.xlsx]]&lt;br /&gt;
# Output (within powerpoint presentation): [[Media:Wt_profileimage_presentation_emmat.pptx]]&lt;br /&gt;
&lt;br /&gt;
===Electronic notebook corresponding to microarray results===&lt;br /&gt;
# [[Emmatyrnauer Week 8]]&lt;br /&gt;
# [[Emmatyrnauer Week 10]]&lt;br /&gt;
# [[Emmatyrnauer Week 14]]&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=File:Draft_LCI_Final_Presentation.pdf&amp;diff=5479</id>
		<title>File:Draft LCI Final Presentation.pdf</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=File:Draft_LCI_Final_Presentation.pdf&amp;diff=5479"/>
				<updated>2017-12-06T00:01:15Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: Draft of final presentation made by team Lights, Camera, InterACTION!&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;Draft of final presentation made by team Lights, Camera, InterACTION!&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Template:Pugs_Not_Drugs&amp;diff=5248</id>
		<title>Template:Pugs Not Drugs</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Template:Pugs_Not_Drugs&amp;diff=5248"/>
				<updated>2017-11-30T23:09:54Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Navigational Links */ changed a link name&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Navigational Links==&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;Background: honeydew&amp;quot;&lt;br /&gt;
|- &lt;br /&gt;
!style=&amp;quot;background: mediumseagreen;color:white&amp;quot;| Category &lt;br /&gt;
!style=&amp;quot;background: mediumseagreen;color:white&amp;quot;| Links&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Interwiki&amp;#039;&amp;#039;&amp;#039; ||style=&amp;quot;width:575px&amp;quot;| [[Main Page]] &amp;amp;emsp; [[Main_Page#Course_Schedule|Course schedule]] &amp;amp;emsp; [[Lights, Camera, InterACTION!]] &amp;amp;emsp; [[Lights, Camera, InterACTION! Deliverables|LCI! Deliverables]]&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Team Members&amp;#039;&amp;#039;&amp;#039; ||  [[User:Bhamilton18|Blair Hamilton]] &amp;amp;emsp; [[user:emmatyrnauer|Emma Tyrnauer]] &amp;amp;emsp; [[User:Kwrigh35|Katie Wright]] &amp;amp;emsp; [[user:zvanysse|Zach Van Ysseldyk]]&lt;br /&gt;
|-&lt;br /&gt;
| &amp;#039;&amp;#039;&amp;#039;GRNsight Gene Page&amp;#039;&amp;#039;&amp;#039; &amp;lt;br&amp;gt;  &amp;#039;&amp;#039;&amp;#039;Project Instructions&amp;#039;&amp;#039;&amp;#039; ||style=&amp;quot;width:500px&amp;quot;| [[GRNsight Gene Page Project | Main Instructions]] &amp;amp;emsp; [[GRNsight Gene Page Project Deliverables | Project Deliverables]] &amp;amp;emsp; [[Week 11]] &amp;amp;emsp; [[Week 12]] &amp;amp;emsp; [[Week 14]] &amp;amp;emsp; [[Week 15]]&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Guilds&amp;#039;&amp;#039;&amp;#039; || [[Project Manager]] &amp;amp;emsp; [[Quality Assurance]] &amp;amp;emsp; [[Data Analysis]] &amp;amp;emsp; [[Coder]]&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;GitHub GRNsight Link&amp;#039;&amp;#039;&amp;#039; || https://github.com/bhamilton18/GRNsight&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
[[category:Group Projects]]&lt;br /&gt;
[[category:Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5244</id>
		<title>Lights, Camera, InterACTION! Deliverables</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5244"/>
				<updated>2017-11-30T23:08:20Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: fixed template name&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;===Group Report and Presentation===&lt;br /&gt;
&lt;br /&gt;
===Individual Statements===&lt;br /&gt;
&lt;br /&gt;
===Code and README===&lt;br /&gt;
&lt;br /&gt;
===ANOVA and stem results===&lt;br /&gt;
&lt;br /&gt;
===Gene List and GO list files===&lt;br /&gt;
&lt;br /&gt;
===YEASTRACT results===&lt;br /&gt;
&lt;br /&gt;
===GRNmap input and output workbooks===&lt;br /&gt;
&lt;br /&gt;
===Electronic notebook corresponding to microarray results===&lt;br /&gt;
&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5240</id>
		<title>Lights, Camera, InterACTION! Deliverables</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5240"/>
				<updated>2017-11-30T23:06:07Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: fixed syntax&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;===Group Report and Presentation===&lt;br /&gt;
&lt;br /&gt;
===Individual Statements===&lt;br /&gt;
&lt;br /&gt;
===Code and README===&lt;br /&gt;
&lt;br /&gt;
===ANOVA and stem results===&lt;br /&gt;
&lt;br /&gt;
===Gene List and GO list files===&lt;br /&gt;
&lt;br /&gt;
===YEASTRACT results===&lt;br /&gt;
&lt;br /&gt;
===GRNmap input and output workbooks===&lt;br /&gt;
&lt;br /&gt;
===Electronic notebook corresponding to microarray results===&lt;br /&gt;
&lt;br /&gt;
{{Lights, Camera, InterACTION!}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5239</id>
		<title>Lights, Camera, InterACTION! Deliverables</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!_Deliverables&amp;diff=5239"/>
				<updated>2017-11-30T23:05:44Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: Created page, added team template and sections&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;===Group Report and Presentation===&lt;br /&gt;
&lt;br /&gt;
===Individual Statements===&lt;br /&gt;
&lt;br /&gt;
===Code and README===&lt;br /&gt;
&lt;br /&gt;
===ANOVA and stem results===&lt;br /&gt;
&lt;br /&gt;
===Gene List and GO list files===&lt;br /&gt;
&lt;br /&gt;
===YEASTRACT results===&lt;br /&gt;
&lt;br /&gt;
===GRNmap input and output workbooks===&lt;br /&gt;
&lt;br /&gt;
===Electronic notebook corresponding to microarray results===&lt;br /&gt;
&lt;br /&gt;
{{Lights, Camera, InterACTION!&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Template:Pugs_Not_Drugs&amp;diff=5232</id>
		<title>Template:Pugs Not Drugs</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Template:Pugs_Not_Drugs&amp;diff=5232"/>
				<updated>2017-11-30T22:58:22Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Navigational Links */ added link for our deliverable&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Navigational Links==&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;Background: honeydew&amp;quot;&lt;br /&gt;
|- &lt;br /&gt;
!style=&amp;quot;background: mediumseagreen;color:white&amp;quot;| Category &lt;br /&gt;
!style=&amp;quot;background: mediumseagreen;color:white&amp;quot;| Links&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Interwiki&amp;#039;&amp;#039;&amp;#039; ||style=&amp;quot;width:575px&amp;quot;| [[Main Page]] &amp;amp;emsp; [[Main_Page#Course_Schedule|Course schedule]] &amp;amp;emsp; [[Lights, Camera, InterACTION!]] &amp;amp;emsp; [[Lights, Camera, InterACTION! Deliverables]]&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Team Members&amp;#039;&amp;#039;&amp;#039; ||  [[User:Bhamilton18|Blair Hamilton]] &amp;amp;emsp; [[user:emmatyrnauer|Emma Tyrnauer]] &amp;amp;emsp; [[User:Kwrigh35|Katie Wright]] &amp;amp;emsp; [[user:zvanysse|Zach Van Ysseldyk]]&lt;br /&gt;
|-&lt;br /&gt;
| &amp;#039;&amp;#039;&amp;#039;GRNsight Gene Page&amp;#039;&amp;#039;&amp;#039; &amp;lt;br&amp;gt;  &amp;#039;&amp;#039;&amp;#039;Project Instructions&amp;#039;&amp;#039;&amp;#039; ||style=&amp;quot;width:500px&amp;quot;| [[GRNsight Gene Page Project | Main Instructions]] &amp;amp;emsp; [[GRNsight Gene Page Project Deliverables | Project Deliverables]] &amp;amp;emsp; [[Week 11]] &amp;amp;emsp; [[Week 12]] &amp;amp;emsp; [[Week 14]] &amp;amp;emsp; [[Week 15]]&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Guilds&amp;#039;&amp;#039;&amp;#039; || [[Project Manager]] &amp;amp;emsp; [[Quality Assurance]] &amp;amp;emsp; [[Data Analysis]] &amp;amp;emsp; [[Coder]]&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;GitHub GRNsight Link&amp;#039;&amp;#039;&amp;#039; || https://github.com/bhamilton18/GRNsight&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
[[category:Group Projects]]&lt;br /&gt;
[[category:Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_14&amp;diff=5169</id>
		<title>Kwrigh35 Week 14</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_14&amp;diff=5169"/>
				<updated>2017-11-28T23:57:32Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* General Info Needed on Each Gene Page */ added to list&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==General Info Needed on Each Gene Page==&lt;br /&gt;
&lt;br /&gt;
Gene ID for all 5 databases&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;NCBI&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*systematic name (locus tag)&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;ENsembl&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*Name&lt;br /&gt;
*DNA sequence&lt;br /&gt;
*descripton of function&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;UniProt&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*Protein name&lt;br /&gt;
*protein sequence&lt;br /&gt;
*Gene Ontology&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;JASPAR&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*Sequence Logo&lt;br /&gt;
*Frequency Matrix&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;SGD&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*SGD ID&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_14&amp;diff=5164</id>
		<title>Kwrigh35 Week 14</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_14&amp;diff=5164"/>
				<updated>2017-11-28T23:54:30Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: created page, first section&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==General Info Needed on Each Gene Page==&lt;br /&gt;
&lt;br /&gt;
Gene ID for all 5 databases&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;NCBI&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
--systematic name (locus tag)&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;ENsembl&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*Name&lt;br /&gt;
*DNA sequence&lt;br /&gt;
*descripton of function&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;UniProt&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*Protein name&lt;br /&gt;
*protein sequence&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;JASPAR&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*Sequence Logo&lt;br /&gt;
*Frequency Matrix&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;SGD&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*SGD ID&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5061</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5061"/>
				<updated>2017-11-21T07:57:50Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Emma and Katie&amp;#039;s personal ideas on further research */ added a few more points (top three)&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===Link to Presentation PDF===&lt;br /&gt;
[[media:Genome-wide expression analysis of yeast response during exposure to 4 degrees C.pdf|Genome-wide expression analysis of yeast response during exposure to 4 degrees C]]&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
*Cold shock response in yeast has been researched quite a bit, but the authors claim that this work is the first to study the effects at 4&amp;amp;deg;C. The Authors believe that their work is the first to study cold-shock response in yeast at a temperature below 10&amp;amp;deg;C.  &lt;br /&gt;
*It is important to study organisms at low temperatures because many organisms are exposed to freezing or near-freezing temperatures in the natural world.  Some organisms (such as &amp;#039;&amp;#039;E. coli&amp;#039;&amp;#039;) are quite often exposed to these low temperatures during experimental preparation.  &lt;br /&gt;
*It is also important to study the differences between organisms that have never been exposed to cold temperatures and organisms that have been grown in these cold conditions.  This will allow us to determine if/how well yeast can adapt to the cold.&lt;br /&gt;
&lt;br /&gt;
====Methods and Materials====&lt;br /&gt;
=====Preparation of yeast and probes=====&lt;br /&gt;
*Strain S288c was grown in the following conditions:&lt;br /&gt;
**Grown at 25°C until mid-log phase &lt;br /&gt;
**Cooled to 4°C and harvested after 6, 12, 24 &amp;amp; 48 h&lt;br /&gt;
**2 Controls grown continuously in 4°C &amp;amp; 35°C environments.&lt;br /&gt;
*RNA was extracted by the hot-phenol method. &lt;br /&gt;
*mRNA was purified with an mRNA purification kit. &lt;br /&gt;
*The probes were prepared by mixing 1-2μg of mRNA with two primers and  a dNTP nucleotide mix.  &lt;br /&gt;
**Reverse transcribed cDNA&lt;br /&gt;
**Probes were purified using a G-50 column&lt;br /&gt;
&lt;br /&gt;
=====Experimental design of the microarray data=====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
*Growth in yeast cells slowed once they were moved from 25&amp;amp;deg;C to 4&amp;amp;deg;C.    &lt;br /&gt;
*Hierarchical cluster analysis can be used to determine which sets of data are most similar to each other.  The process begins by grouping the two most similar data sets together into a &amp;quot;cluster.&amp;quot; Data sets are then added one-by-one to the initial cluster in order of similarity, until all data is categorized into one cluster. &amp;#039;&amp;#039;This technique revealed which time-points have the greatest similarity in gene expression.&amp;#039;&amp;#039;&lt;br /&gt;
**Hours 12 and 24 have the most similar gene-expression profile, and therefore are clustered together first.  &lt;br /&gt;
**The next closest to this cluster is 48 hours, which can then be added to the 12/24h  cluster form another, bigger cluster.  &lt;br /&gt;
**Hour 6 is the next closest to this cluster. &lt;br /&gt;
**Then the expression profile of the yeast constantly exposed to 4&amp;amp;deg;C &lt;br /&gt;
**And finally, with the least similar gene expression, is the yeast constantly exposed to 35&amp;amp;deg;C.&lt;br /&gt;
*Up and down-regulation of genes occurred according to what functions were needed for cell viability&lt;br /&gt;
**Genes involved in cell rescue, defense and virulence, and energy and metabolism were up-regulated &lt;br /&gt;
**Genes involved in protein synthesis, binding functions, activity regulation and fate were down-regulated&lt;br /&gt;
*Transcription of some genes go into overdrive after cold shock, others don’t&lt;br /&gt;
**Cold-shock causes up-regulation of genes that lead to&lt;br /&gt;
*** Energy preservation and cold tolerance&lt;br /&gt;
*** Membrane maintenance and permeability increases&lt;br /&gt;
***Detoxification of active oxygen species&lt;br /&gt;
***Revitalization of enzyme activity&lt;br /&gt;
**Causes down-regulation of genes that lead to growth&lt;br /&gt;
*** Allows yeast to adapt to new environment&lt;br /&gt;
&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
The results of this study looked very &amp;#039;&amp;#039;&amp;#039;similar&amp;#039;&amp;#039;&amp;#039; to those of previous studies.  Here are a couple of examples:&lt;br /&gt;
*Genes encoding HSPs were induced by 4&amp;amp;deg;C exposure.  These genes were also observed to be induced at 0&amp;amp;deg;C during a 2004 study of near-freezing response by Kang et al.  &lt;br /&gt;
*Trehalose synthesis was also shown to be important at low temperatures during a 2004 study by Kandror et al.  &lt;br /&gt;
&lt;br /&gt;
There are also some instances where the results of this study are &amp;#039;&amp;#039;&amp;#039;different&amp;#039;&amp;#039;&amp;#039; from previous ones.  For example:&lt;br /&gt;
*TPS1 and TPS2 genes were found to have a smaller fold induction in this study compared to a previous study by Kandror et al in 2004. &lt;br /&gt;
**This discrepancy may be explained by use of different carbon sources in the two studies.&lt;br /&gt;
&lt;br /&gt;
====Emma and Katie&amp;#039;s personal ideas on further research====&lt;br /&gt;
*It was somewhat frustrating that this paper made so many references to previous papers, and yet did not compare its results to very many.  &lt;br /&gt;
*The paper also did not indicate what the authors wanted to pursue as far as future research&lt;br /&gt;
*Because of the previous two points, Emma and I decided to suggest the following two ideas for future research:&lt;br /&gt;
**How different types of stresses lead to similar or different changes in gene expression (e.g. salinity, pressure, chemicals, etc)&lt;br /&gt;
**If there is significant change in gene expression depending on the temperature used for cold shock (ie cold shock at 2°C, 4°C and 6°C and 8°C).&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=5057</id>
		<title>Lights, Camera, InterACTION!</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=5057"/>
				<updated>2017-11-21T07:51:10Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: added &amp;quot;PM:&amp;quot; where missing&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:PugNotDrugsLogo.png|400px|thumb|right|Pugs Not Drugs Logo]]&lt;br /&gt;
&lt;br /&gt;
==Team Members==&lt;br /&gt;
*[[user:bhamilton18|Blair Hamilton]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:emmatyrnauer|Emma Tyrnauer]] &amp;#039;&amp;#039;&amp;#039;-Data Analyst&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:kwrigh35|Katie Wright]] &amp;#039;&amp;#039;&amp;#039;-Project Manager/Quality Assurance&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:zvanysse|Zach Van Ysseldyke]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
==File Roundup==&lt;br /&gt;
*Week 11 presentation by Blair and Zach: [[media:The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf|The Secret Startup that Saved the Worst Website in America]]&lt;br /&gt;
*Week 12 presentation by Emma and Katie: [[media:Genome-wide expression analysis of yeast response during exposure to 4 degrees C.pdf|Genome-wide expression analysis of yeast response during exposure to 4 degrees C]]&lt;br /&gt;
&lt;br /&gt;
==Team Schedule== &amp;lt;!--Borrowed Format from JASPAR group--&amp;gt;&lt;br /&gt;
{| class =&amp;quot;wikitable&amp;quot;&lt;br /&gt;
|+November &amp;amp; December&lt;br /&gt;
|-&lt;br /&gt;
|Sunday&lt;br /&gt;
|Monday&lt;br /&gt;
|Tuesday&lt;br /&gt;
|Wednesday&lt;br /&gt;
|Thursday&lt;br /&gt;
|Friday&lt;br /&gt;
|Saturday&lt;br /&gt;
|-&lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt; C: PPT Presentation&amp;lt;br&amp;gt;PM/DA: Journal Articles&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;17&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;18&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;19&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;20&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;21&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;C: Complete setup steps (Milestones 0-4)&amp;lt;br&amp;gt;PM/DA: Journal Club Presentations&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;22&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;23&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;24&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;25&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;26&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;27&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;28&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: First Integration projected completion&amp;lt;br&amp;gt;PM: Check in with team &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;29&amp;#039;&amp;#039;&amp;#039; &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;30&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM: Check in with team &amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with groups, and further integrate as needed.&amp;lt;br&amp;gt;PM: Check in with team &amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM: Check in with team &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;Final group Presentation &amp;#039;&amp;#039;2:00-4:00PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;All deliverables due &amp;#039;&amp;#039;4:30PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
==Executive Summaries==&lt;br /&gt;
===Week 11===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
For this week, I worked with [[user:emmatyrnauer|Emma]] to find papers to use as reference in our final research paper.  Emma and I both found papers that are related to yeast cold shock reactions and most of them use microarray data.  I am excited to read these articles more in depth as we develop our research project and paper.  Further information can be found on my [[kwrigh35 Week 11|Week 11]] journal page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039; Using the three different sources was really helpful because I had never used &amp;quot;Web of Science&amp;quot; before.  It was good to learn about how different these results can be, especially with tweaking the search terms a little bit.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I had poor time management this week.  I waited until Monday to do the majority of the work and definitely should not have done so.  The rest of the project went well, however.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I will plan to meet up with Emma early on in the week so we can work together on our presentation.  This way I will be held accountable to get my work done earlier.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week Katie and I worked on the annotated bibliography (below). We chose papers that provided more information on yeast gene regulation in response to cold shock. Pub Med, Google Scholar, and Web of Science were utilized to perform basic searches using key words as well as advanced searches which helped to narrow the results. More detailed information can be found on my individual journal entry page: [[Emmatyrnauer Week 11]]. Meanwhile, Blair and Zach worked on coder journal club presentation. Construction of the group page was completed during class.&lt;br /&gt;
# Overall, this week went pretty smoothly; Katie and I communicated over text to determine which papers we were going to write the bibliographies for and were available to each other for any questions we had.&lt;br /&gt;
# There wasn&amp;#039;t really anything that didn&amp;#039;t work.&lt;br /&gt;
# Since we will be working this next week on our journal club presentation, it might be beneficial to meet in person.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:26, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
This week Zach and I created the Powerpoint presentation about &amp;#039;&amp;#039;The secret startup that saved the worst website in America&amp;#039;&amp;#039; article. We created individual outlines of the article on our respective pages, mine is as follows: [[Bhamilton18 Week 11]]. Our presentation is linked here: [[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]]&lt;br /&gt;
#Overall, our communication and diving up of tasks went well and smoothly. Meanwhile, Katie and Emma worked on the annotate bibliography. While I primarily worked with Zach our communication amongst group members went well and we hope to continue to do so for the future.&lt;br /&gt;
#Something that didn&amp;#039;t work as well was our time management. Next time we hope to have more class time and start working earlier on our portion of the assignment. &lt;br /&gt;
#For the future, we will continue to communicate in person and through messaging and will work on time management by starting earlier.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 23:35, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
[[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]] &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 11]]&amp;lt;br&amp;gt;&lt;br /&gt;
This week, Blair and I created a Powerpoint about the &amp;quot;Secret Startup that saved the worst website in America.&amp;quot; We read through the article and picked out the relevant themes and main messages to put on the PowerPoint. We wrote these themes and summaries out on our individual Journals. Then, we collaborated and made our PowerPoint. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; Blair and I&amp;#039;s communication was very strong and we knew exactly what was going on with one another. We knew when we were going to meet up and what was expected for each of us.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Blair and I missed the mark on our time management. We did not expect this assignment to take this long. Next time, we will make sure to start on assignments earlier.&lt;br /&gt;
#Like I said above, we will plan out exact times earlier on in the week that we can meet up&lt;br /&gt;
[[User:Zvanysse|Zvanysse]] ([[User talk:Zvanysse|talk]]) 23:44, 13 November 2017 (PST) &amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
===Week 12===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
Emma and I worked on our presentation on &amp;quot;Genome-wide expression analysis of yeast response during exposure to 4 degrees C,&amp;quot; details of which can be found on my [[kwrigh35 Week 12|Week 12]] page.  The presentation can be found in the &amp;quot;file roundup&amp;quot; section of this page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039;Emma and I met up outside of class to work on the assignment, which was really helpful.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I underestimated to the time it would take to find 10 terms that I didn&amp;#039;t know before reading the paper.  It was hard to find 10, so I resorted to picking terms that I knew but wanted to know more about.  It was also difficult to find definitions for some of these words, because some of them weren&amp;#039;t in biological dictionaries.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I should start work on the assignment before the weekend begins.  That way, I don&amp;#039;t have to spend the majority of my Monday afternoon/evening on the project.  It is better to be able to come back to a project several times, and each time have &amp;quot;fresh&amp;quot; eyes.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week I made all the corrections listed on the talk page of my user page for both [[Emmatyrnauer Week 8]] and [[Emmatyrnauer Week 10]]. This included corrections to the excel files, stem analysis, electronic notebooks, analysis of log fold changes and p values, and the acknowledgements section. Following the completion of fixing the errors in the excel file, I emailed Dr. Dahlquist to ascertain that I completed the analysis correctly. For the individual assignment this week, Katie and I met up to work on our journal club presentation of the paper that we were assigned (can be accessed on my individual journal page, [[Emmatyrnauer Week 12]]. We divided up what portions we would be responsible for and have planned to meet up before class tomorrow to practice.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; It was very helpful to meet up in person with Katie to begin working on the powerpoint before separating everything out.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; I could have been in better communication with Blair and Zach this week.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Sitting down together as a group will help to get us all on the same page for the future weeks.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:03, 20 November 2017 (PST)&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
As mentioned on my electronic notebook, [[Bhamilton18 Week 12]], this week&amp;#039;s assignment Zachary Van Ysseldyk and I worked on the Coder Milestones 0-4. Zach and I will be working on the integration of the website bringing together the other groups work to function on the GRNsight website. Katie and Emma worked on their respective journal club presentation, but we communicated to check base on how everyone is doing in the progression of the project. Zach and I met in person to collaborate on formatting of the team page, individual journal pages and project progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; Zach and I worked on better time management as well as expectations for the assignment. We were able to communicate without any hiccups, and meeting up in person was ideal for clarifying issues.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; We were unsure of our progress in the project as our understanding of milestone 4 meant we needed to wait this week on others. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Checking in with our project teams/coders will help us understand how they are doing, as well as how our own progression is lining up.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 19:15, 20 November 2017 (PST)&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
This week, Blair and I worked on the coder milestones. We will work on integrating the actual other team&amp;#039;s parts as they start to finish. We also collaborated with Emma and Katie to make sure that we were on the same page. &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 12]]&amp;lt;br&amp;gt;&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; we were better about our time management as well as we communicated well. We met up together periodically to make sure that we were on the same page.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; We were a little confused on how to go about the project for our milestone considering that it was more of a waiting game this week rather than actual integration. So, we didn&amp;#039;t really know how to quantify our progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What we will do to fix it:&amp;#039;&amp;#039;&amp;#039; We maybe should have collaborated with the other teams a little bit more to be able and gauge how their projects are coming along.&lt;br /&gt;
&amp;lt;!----&lt;br /&gt;
===Week 14===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
===Week 15===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
---&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Bibliography==&lt;br /&gt;
===Paper 1===&lt;br /&gt;
Homma, T., Iwahashi, H., &amp;amp; Komatsu, Y. (2003). Yeast gene expression during growth at low temperature. &amp;#039;&amp;#039;Cryobiology&amp;#039;&amp;#039;, 46(3), 230-237. &lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/12818212&lt;br /&gt;
* PubMed Central:  not available&lt;br /&gt;
* Publisher Full Text (HTML):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=12818212&lt;br /&gt;
* Publisher Full Text (PDF): https://electra.lmu.edu:3555/S0011224003000282/1-s2.0-S0011224003000282-main.pdf?_tid=4c9b7cc0-c775-11e7-af71-00000aab0f26&amp;amp;acdnat=1510469407_3e02bee4266fd6a211ea3f6e27b460cf &lt;br /&gt;
* Copyright: 2003 by Science Direct; article is not Open Access&lt;br /&gt;
* Publisher:  Elsevier Science&lt;br /&gt;
* Availability: online at www.sciencedirect.com&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 2===&lt;br /&gt;
Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., ... &amp;amp; Fujita, K. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. &amp;#039;&amp;#039;Extremophiles&amp;#039;&amp;#039;, 10(2), 117-128.&lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/?term=Genome-wide+expression+analysis+of+yeast+response+during+exposure+to+4+degrees+C&lt;br /&gt;
* PubMed Central: not available&lt;br /&gt;
* Publisher Full Text (HTML): https://electra.lmu.edu:2529/article/10.1007/s00792-005-0480-1&lt;br /&gt;
* Publisher Full Text (PDF):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=16254683&lt;br /&gt;
* Copyright: 2005 by Springer-Verlag; article is not Open Access&lt;br /&gt;
* Publisher:  Springer-Verlag&lt;br /&gt;
* Availability: online&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 3===&lt;br /&gt;
Becerra, M., Lombardia, L. J., González‐Siso, M. I., Rodríguez‐Belmonte, E., Hauser, N. C., &amp;amp; Cerdán, M. E. (2003). Genome‐wide analysis of the yeast transcriptome upon heat and cold shock. Comparative and functional genomics, 4(4), 366-375.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/pdf/CFG-04-366.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML):&amp;#039;&amp;#039;&amp;#039; unavailable &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF):&amp;#039;&amp;#039;&amp;#039; http://downloads.hindawi.com/journals/ijg/2003/175356.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright:&amp;#039;&amp;#039;&amp;#039; Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher:&amp;#039;&amp;#039;&amp;#039; Hindawi Publishing Corporation&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability:&amp;#039;&amp;#039;&amp;#039; Open access&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; no&lt;br /&gt;
&lt;br /&gt;
===Paper 4===&lt;br /&gt;
Sahara, T., Goda, T., &amp;amp; Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract: &amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pubmed/?term=Comprehensive+expression+analysis+of+time-dependent+genetic+responses+in+yeast+cells+to+low+temperature&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central: &amp;#039;&amp;#039;&amp;#039; not available &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.long&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.full.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright: &amp;#039;&amp;#039;&amp;#039; &amp;quot;Other parties are welcome to copy, distribute, transmit and adapt the work — at no cost and without permission — for noncommercial use as long as they attribute the work to the original source using the citation above.&amp;quot; from http://www.jbc.org/site/misc/Copyright_Permission.xhtml&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher: &amp;#039;&amp;#039;&amp;#039; American Society for Biochemistry and Molecular Biology&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability: &amp;#039;&amp;#039;&amp;#039;All articles from this journal are free 1 year after publishing.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; No.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Acknowledgments==&lt;br /&gt;
#Our group collaborates on this page each week filling out our respective team journal assignments and project updates.&lt;br /&gt;
#*WE have a group chat to communicate problems, questions and ideas for our weekly assignments/milestones.&lt;br /&gt;
#We utilized the Calendar outline from the [[JASPAR the Friendly Ghost]] page, but changed the milestone/tasks due.&lt;br /&gt;
&lt;br /&gt;
==References==&lt;br /&gt;
*Meyer, R. (2015, July 09). The Secret Startup That Saved the Worst Website in America. Retrieved November 7, 2017, from https://www.theatlantic.com/technology/archive/2015/07/the-secret-startup- saved-healthcare-gov-the-worst-website-in-america/397784/&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;br /&gt;
&lt;br /&gt;
{{GRNsight Gene Page Project Links}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=5054</id>
		<title>Lights, Camera, InterACTION!</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=5054"/>
				<updated>2017-11-21T07:50:07Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: added info for Dec 5&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:PugNotDrugsLogo.png|400px|thumb|right|Pugs Not Drugs Logo]]&lt;br /&gt;
&lt;br /&gt;
==Team Members==&lt;br /&gt;
*[[user:bhamilton18|Blair Hamilton]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:emmatyrnauer|Emma Tyrnauer]] &amp;#039;&amp;#039;&amp;#039;-Data Analyst&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:kwrigh35|Katie Wright]] &amp;#039;&amp;#039;&amp;#039;-Project Manager/Quality Assurance&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:zvanysse|Zach Van Ysseldyke]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
==File Roundup==&lt;br /&gt;
*Week 11 presentation by Blair and Zach: [[media:The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf|The Secret Startup that Saved the Worst Website in America]]&lt;br /&gt;
*Week 12 presentation by Emma and Katie: [[media:Genome-wide expression analysis of yeast response during exposure to 4 degrees C.pdf|Genome-wide expression analysis of yeast response during exposure to 4 degrees C]]&lt;br /&gt;
&lt;br /&gt;
==Team Schedule== &amp;lt;!--Borrowed Format from JASPAR group--&amp;gt;&lt;br /&gt;
{| class =&amp;quot;wikitable&amp;quot;&lt;br /&gt;
|+November &amp;amp; December&lt;br /&gt;
|-&lt;br /&gt;
|Sunday&lt;br /&gt;
|Monday&lt;br /&gt;
|Tuesday&lt;br /&gt;
|Wednesday&lt;br /&gt;
|Thursday&lt;br /&gt;
|Friday&lt;br /&gt;
|Saturday&lt;br /&gt;
|-&lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt; C: PPT Presentation&amp;lt;br&amp;gt;PM/DA: Journal Articles&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;17&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;18&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;19&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;20&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;21&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;C: Complete setup steps (Milestones 0-4)&amp;lt;br&amp;gt;PM/DA: Journal Club Presentations&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;22&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;23&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;24&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;25&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;26&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;27&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;28&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: First Integration projected completion&amp;lt;br&amp;gt;PM:Check in with team &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;29&amp;#039;&amp;#039;&amp;#039; &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;30&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM:Check in with team &amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with groups, and further integrate as needed.&amp;lt;br&amp;gt; Check in with team &amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM:Check in with team &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;Final group Presentation &amp;#039;&amp;#039;2:00-4:00PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;All deliverables due &amp;#039;&amp;#039;4:30PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
==Executive Summaries==&lt;br /&gt;
===Week 11===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
For this week, I worked with [[user:emmatyrnauer|Emma]] to find papers to use as reference in our final research paper.  Emma and I both found papers that are related to yeast cold shock reactions and most of them use microarray data.  I am excited to read these articles more in depth as we develop our research project and paper.  Further information can be found on my [[kwrigh35 Week 11|Week 11]] journal page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039; Using the three different sources was really helpful because I had never used &amp;quot;Web of Science&amp;quot; before.  It was good to learn about how different these results can be, especially with tweaking the search terms a little bit.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I had poor time management this week.  I waited until Monday to do the majority of the work and definitely should not have done so.  The rest of the project went well, however.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I will plan to meet up with Emma early on in the week so we can work together on our presentation.  This way I will be held accountable to get my work done earlier.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week Katie and I worked on the annotated bibliography (below). We chose papers that provided more information on yeast gene regulation in response to cold shock. Pub Med, Google Scholar, and Web of Science were utilized to perform basic searches using key words as well as advanced searches which helped to narrow the results. More detailed information can be found on my individual journal entry page: [[Emmatyrnauer Week 11]]. Meanwhile, Blair and Zach worked on coder journal club presentation. Construction of the group page was completed during class.&lt;br /&gt;
# Overall, this week went pretty smoothly; Katie and I communicated over text to determine which papers we were going to write the bibliographies for and were available to each other for any questions we had.&lt;br /&gt;
# There wasn&amp;#039;t really anything that didn&amp;#039;t work.&lt;br /&gt;
# Since we will be working this next week on our journal club presentation, it might be beneficial to meet in person.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:26, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
This week Zach and I created the Powerpoint presentation about &amp;#039;&amp;#039;The secret startup that saved the worst website in America&amp;#039;&amp;#039; article. We created individual outlines of the article on our respective pages, mine is as follows: [[Bhamilton18 Week 11]]. Our presentation is linked here: [[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]]&lt;br /&gt;
#Overall, our communication and diving up of tasks went well and smoothly. Meanwhile, Katie and Emma worked on the annotate bibliography. While I primarily worked with Zach our communication amongst group members went well and we hope to continue to do so for the future.&lt;br /&gt;
#Something that didn&amp;#039;t work as well was our time management. Next time we hope to have more class time and start working earlier on our portion of the assignment. &lt;br /&gt;
#For the future, we will continue to communicate in person and through messaging and will work on time management by starting earlier.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 23:35, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
[[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]] &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 11]]&amp;lt;br&amp;gt;&lt;br /&gt;
This week, Blair and I created a Powerpoint about the &amp;quot;Secret Startup that saved the worst website in America.&amp;quot; We read through the article and picked out the relevant themes and main messages to put on the PowerPoint. We wrote these themes and summaries out on our individual Journals. Then, we collaborated and made our PowerPoint. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; Blair and I&amp;#039;s communication was very strong and we knew exactly what was going on with one another. We knew when we were going to meet up and what was expected for each of us.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Blair and I missed the mark on our time management. We did not expect this assignment to take this long. Next time, we will make sure to start on assignments earlier.&lt;br /&gt;
#Like I said above, we will plan out exact times earlier on in the week that we can meet up&lt;br /&gt;
[[User:Zvanysse|Zvanysse]] ([[User talk:Zvanysse|talk]]) 23:44, 13 November 2017 (PST) &amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
===Week 12===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
Emma and I worked on our presentation on &amp;quot;Genome-wide expression analysis of yeast response during exposure to 4 degrees C,&amp;quot; details of which can be found on my [[kwrigh35 Week 12|Week 12]] page.  The presentation can be found in the &amp;quot;file roundup&amp;quot; section of this page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039;Emma and I met up outside of class to work on the assignment, which was really helpful.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I underestimated to the time it would take to find 10 terms that I didn&amp;#039;t know before reading the paper.  It was hard to find 10, so I resorted to picking terms that I knew but wanted to know more about.  It was also difficult to find definitions for some of these words, because some of them weren&amp;#039;t in biological dictionaries.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I should start work on the assignment before the weekend begins.  That way, I don&amp;#039;t have to spend the majority of my Monday afternoon/evening on the project.  It is better to be able to come back to a project several times, and each time have &amp;quot;fresh&amp;quot; eyes.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week I made all the corrections listed on the talk page of my user page for both [[Emmatyrnauer Week 8]] and [[Emmatyrnauer Week 10]]. This included corrections to the excel files, stem analysis, electronic notebooks, analysis of log fold changes and p values, and the acknowledgements section. Following the completion of fixing the errors in the excel file, I emailed Dr. Dahlquist to ascertain that I completed the analysis correctly. For the individual assignment this week, Katie and I met up to work on our journal club presentation of the paper that we were assigned (can be accessed on my individual journal page, [[Emmatyrnauer Week 12]]. We divided up what portions we would be responsible for and have planned to meet up before class tomorrow to practice.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; It was very helpful to meet up in person with Katie to begin working on the powerpoint before separating everything out.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; I could have been in better communication with Blair and Zach this week.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Sitting down together as a group will help to get us all on the same page for the future weeks.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:03, 20 November 2017 (PST)&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
As mentioned on my electronic notebook, [[Bhamilton18 Week 12]], this week&amp;#039;s assignment Zachary Van Ysseldyk and I worked on the Coder Milestones 0-4. Zach and I will be working on the integration of the website bringing together the other groups work to function on the GRNsight website. Katie and Emma worked on their respective journal club presentation, but we communicated to check base on how everyone is doing in the progression of the project. Zach and I met in person to collaborate on formatting of the team page, individual journal pages and project progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; Zach and I worked on better time management as well as expectations for the assignment. We were able to communicate without any hiccups, and meeting up in person was ideal for clarifying issues.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; We were unsure of our progress in the project as our understanding of milestone 4 meant we needed to wait this week on others. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Checking in with our project teams/coders will help us understand how they are doing, as well as how our own progression is lining up.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 19:15, 20 November 2017 (PST)&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
This week, Blair and I worked on the coder milestones. We will work on integrating the actual other team&amp;#039;s parts as they start to finish. We also collaborated with Emma and Katie to make sure that we were on the same page. &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 12]]&amp;lt;br&amp;gt;&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; we were better about our time management as well as we communicated well. We met up together periodically to make sure that we were on the same page.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; We were a little confused on how to go about the project for our milestone considering that it was more of a waiting game this week rather than actual integration. So, we didn&amp;#039;t really know how to quantify our progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What we will do to fix it:&amp;#039;&amp;#039;&amp;#039; We maybe should have collaborated with the other teams a little bit more to be able and gauge how their projects are coming along.&lt;br /&gt;
&amp;lt;!----&lt;br /&gt;
===Week 14===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
===Week 15===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
---&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Bibliography==&lt;br /&gt;
===Paper 1===&lt;br /&gt;
Homma, T., Iwahashi, H., &amp;amp; Komatsu, Y. (2003). Yeast gene expression during growth at low temperature. &amp;#039;&amp;#039;Cryobiology&amp;#039;&amp;#039;, 46(3), 230-237. &lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/12818212&lt;br /&gt;
* PubMed Central:  not available&lt;br /&gt;
* Publisher Full Text (HTML):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=12818212&lt;br /&gt;
* Publisher Full Text (PDF): https://electra.lmu.edu:3555/S0011224003000282/1-s2.0-S0011224003000282-main.pdf?_tid=4c9b7cc0-c775-11e7-af71-00000aab0f26&amp;amp;acdnat=1510469407_3e02bee4266fd6a211ea3f6e27b460cf &lt;br /&gt;
* Copyright: 2003 by Science Direct; article is not Open Access&lt;br /&gt;
* Publisher:  Elsevier Science&lt;br /&gt;
* Availability: online at www.sciencedirect.com&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 2===&lt;br /&gt;
Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., ... &amp;amp; Fujita, K. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. &amp;#039;&amp;#039;Extremophiles&amp;#039;&amp;#039;, 10(2), 117-128.&lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/?term=Genome-wide+expression+analysis+of+yeast+response+during+exposure+to+4+degrees+C&lt;br /&gt;
* PubMed Central: not available&lt;br /&gt;
* Publisher Full Text (HTML): https://electra.lmu.edu:2529/article/10.1007/s00792-005-0480-1&lt;br /&gt;
* Publisher Full Text (PDF):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=16254683&lt;br /&gt;
* Copyright: 2005 by Springer-Verlag; article is not Open Access&lt;br /&gt;
* Publisher:  Springer-Verlag&lt;br /&gt;
* Availability: online&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 3===&lt;br /&gt;
Becerra, M., Lombardia, L. J., González‐Siso, M. I., Rodríguez‐Belmonte, E., Hauser, N. C., &amp;amp; Cerdán, M. E. (2003). Genome‐wide analysis of the yeast transcriptome upon heat and cold shock. Comparative and functional genomics, 4(4), 366-375.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/pdf/CFG-04-366.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML):&amp;#039;&amp;#039;&amp;#039; unavailable &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF):&amp;#039;&amp;#039;&amp;#039; http://downloads.hindawi.com/journals/ijg/2003/175356.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright:&amp;#039;&amp;#039;&amp;#039; Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher:&amp;#039;&amp;#039;&amp;#039; Hindawi Publishing Corporation&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability:&amp;#039;&amp;#039;&amp;#039; Open access&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; no&lt;br /&gt;
&lt;br /&gt;
===Paper 4===&lt;br /&gt;
Sahara, T., Goda, T., &amp;amp; Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract: &amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pubmed/?term=Comprehensive+expression+analysis+of+time-dependent+genetic+responses+in+yeast+cells+to+low+temperature&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central: &amp;#039;&amp;#039;&amp;#039; not available &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.long&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.full.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright: &amp;#039;&amp;#039;&amp;#039; &amp;quot;Other parties are welcome to copy, distribute, transmit and adapt the work — at no cost and without permission — for noncommercial use as long as they attribute the work to the original source using the citation above.&amp;quot; from http://www.jbc.org/site/misc/Copyright_Permission.xhtml&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher: &amp;#039;&amp;#039;&amp;#039; American Society for Biochemistry and Molecular Biology&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability: &amp;#039;&amp;#039;&amp;#039;All articles from this journal are free 1 year after publishing.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; No.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Acknowledgments==&lt;br /&gt;
#Our group collaborates on this page each week filling out our respective team journal assignments and project updates.&lt;br /&gt;
#*WE have a group chat to communicate problems, questions and ideas for our weekly assignments/milestones.&lt;br /&gt;
#We utilized the Calendar outline from the [[JASPAR the Friendly Ghost]] page, but changed the milestone/tasks due.&lt;br /&gt;
&lt;br /&gt;
==References==&lt;br /&gt;
*Meyer, R. (2015, July 09). The Secret Startup That Saved the Worst Website in America. Retrieved November 7, 2017, from https://www.theatlantic.com/technology/archive/2015/07/the-secret-startup- saved-healthcare-gov-the-worst-website-in-america/397784/&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;br /&gt;
&lt;br /&gt;
{{GRNsight Gene Page Project Links}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=5053</id>
		<title>Lights, Camera, InterACTION!</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=5053"/>
				<updated>2017-11-21T07:49:08Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: Added PM responsibilities&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:PugNotDrugsLogo.png|400px|thumb|right|Pugs Not Drugs Logo]]&lt;br /&gt;
&lt;br /&gt;
==Team Members==&lt;br /&gt;
*[[user:bhamilton18|Blair Hamilton]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:emmatyrnauer|Emma Tyrnauer]] &amp;#039;&amp;#039;&amp;#039;-Data Analyst&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:kwrigh35|Katie Wright]] &amp;#039;&amp;#039;&amp;#039;-Project Manager/Quality Assurance&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:zvanysse|Zach Van Ysseldyke]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
==File Roundup==&lt;br /&gt;
*Week 11 presentation by Blair and Zach: [[media:The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf|The Secret Startup that Saved the Worst Website in America]]&lt;br /&gt;
*Week 12 presentation by Emma and Katie: [[media:Genome-wide expression analysis of yeast response during exposure to 4 degrees C.pdf|Genome-wide expression analysis of yeast response during exposure to 4 degrees C]]&lt;br /&gt;
&lt;br /&gt;
==Team Schedule== &amp;lt;!--Borrowed Format from JASPAR group--&amp;gt;&lt;br /&gt;
{| class =&amp;quot;wikitable&amp;quot;&lt;br /&gt;
|+November &amp;amp; December&lt;br /&gt;
|-&lt;br /&gt;
|Sunday&lt;br /&gt;
|Monday&lt;br /&gt;
|Tuesday&lt;br /&gt;
|Wednesday&lt;br /&gt;
|Thursday&lt;br /&gt;
|Friday&lt;br /&gt;
|Saturday&lt;br /&gt;
|-&lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt; C: PPT Presentation&amp;lt;br&amp;gt;PM/DA: Journal Articles&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;17&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;18&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;19&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;20&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;21&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;C: Complete setup steps (Milestones 0-4)&amp;lt;br&amp;gt;PM/DA: Journal Club Presentations&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;22&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;23&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;24&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;25&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;26&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;27&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;28&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: First Integration projected completion&amp;lt;br&amp;gt;PM:Check in with team &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;29&amp;#039;&amp;#039;&amp;#039; &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;30&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM:Check in with team &amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with groups, and further integrate as needed.&amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM:Check in with team &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;Final group Presentation &amp;#039;&amp;#039;2:00-4:00PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;All deliverables due &amp;#039;&amp;#039;4:30PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
==Executive Summaries==&lt;br /&gt;
===Week 11===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
For this week, I worked with [[user:emmatyrnauer|Emma]] to find papers to use as reference in our final research paper.  Emma and I both found papers that are related to yeast cold shock reactions and most of them use microarray data.  I am excited to read these articles more in depth as we develop our research project and paper.  Further information can be found on my [[kwrigh35 Week 11|Week 11]] journal page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039; Using the three different sources was really helpful because I had never used &amp;quot;Web of Science&amp;quot; before.  It was good to learn about how different these results can be, especially with tweaking the search terms a little bit.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I had poor time management this week.  I waited until Monday to do the majority of the work and definitely should not have done so.  The rest of the project went well, however.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I will plan to meet up with Emma early on in the week so we can work together on our presentation.  This way I will be held accountable to get my work done earlier.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week Katie and I worked on the annotated bibliography (below). We chose papers that provided more information on yeast gene regulation in response to cold shock. Pub Med, Google Scholar, and Web of Science were utilized to perform basic searches using key words as well as advanced searches which helped to narrow the results. More detailed information can be found on my individual journal entry page: [[Emmatyrnauer Week 11]]. Meanwhile, Blair and Zach worked on coder journal club presentation. Construction of the group page was completed during class.&lt;br /&gt;
# Overall, this week went pretty smoothly; Katie and I communicated over text to determine which papers we were going to write the bibliographies for and were available to each other for any questions we had.&lt;br /&gt;
# There wasn&amp;#039;t really anything that didn&amp;#039;t work.&lt;br /&gt;
# Since we will be working this next week on our journal club presentation, it might be beneficial to meet in person.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:26, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
This week Zach and I created the Powerpoint presentation about &amp;#039;&amp;#039;The secret startup that saved the worst website in America&amp;#039;&amp;#039; article. We created individual outlines of the article on our respective pages, mine is as follows: [[Bhamilton18 Week 11]]. Our presentation is linked here: [[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]]&lt;br /&gt;
#Overall, our communication and diving up of tasks went well and smoothly. Meanwhile, Katie and Emma worked on the annotate bibliography. While I primarily worked with Zach our communication amongst group members went well and we hope to continue to do so for the future.&lt;br /&gt;
#Something that didn&amp;#039;t work as well was our time management. Next time we hope to have more class time and start working earlier on our portion of the assignment. &lt;br /&gt;
#For the future, we will continue to communicate in person and through messaging and will work on time management by starting earlier.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 23:35, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
[[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]] &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 11]]&amp;lt;br&amp;gt;&lt;br /&gt;
This week, Blair and I created a Powerpoint about the &amp;quot;Secret Startup that saved the worst website in America.&amp;quot; We read through the article and picked out the relevant themes and main messages to put on the PowerPoint. We wrote these themes and summaries out on our individual Journals. Then, we collaborated and made our PowerPoint. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; Blair and I&amp;#039;s communication was very strong and we knew exactly what was going on with one another. We knew when we were going to meet up and what was expected for each of us.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Blair and I missed the mark on our time management. We did not expect this assignment to take this long. Next time, we will make sure to start on assignments earlier.&lt;br /&gt;
#Like I said above, we will plan out exact times earlier on in the week that we can meet up&lt;br /&gt;
[[User:Zvanysse|Zvanysse]] ([[User talk:Zvanysse|talk]]) 23:44, 13 November 2017 (PST) &amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
===Week 12===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
Emma and I worked on our presentation on &amp;quot;Genome-wide expression analysis of yeast response during exposure to 4 degrees C,&amp;quot; details of which can be found on my [[kwrigh35 Week 12|Week 12]] page.  The presentation can be found in the &amp;quot;file roundup&amp;quot; section of this page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039;Emma and I met up outside of class to work on the assignment, which was really helpful.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I underestimated to the time it would take to find 10 terms that I didn&amp;#039;t know before reading the paper.  It was hard to find 10, so I resorted to picking terms that I knew but wanted to know more about.  It was also difficult to find definitions for some of these words, because some of them weren&amp;#039;t in biological dictionaries.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I should start work on the assignment before the weekend begins.  That way, I don&amp;#039;t have to spend the majority of my Monday afternoon/evening on the project.  It is better to be able to come back to a project several times, and each time have &amp;quot;fresh&amp;quot; eyes.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week I made all the corrections listed on the talk page of my user page for both [[Emmatyrnauer Week 8]] and [[Emmatyrnauer Week 10]]. This included corrections to the excel files, stem analysis, electronic notebooks, analysis of log fold changes and p values, and the acknowledgements section. Following the completion of fixing the errors in the excel file, I emailed Dr. Dahlquist to ascertain that I completed the analysis correctly. For the individual assignment this week, Katie and I met up to work on our journal club presentation of the paper that we were assigned (can be accessed on my individual journal page, [[Emmatyrnauer Week 12]]. We divided up what portions we would be responsible for and have planned to meet up before class tomorrow to practice.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; It was very helpful to meet up in person with Katie to begin working on the powerpoint before separating everything out.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; I could have been in better communication with Blair and Zach this week.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Sitting down together as a group will help to get us all on the same page for the future weeks.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:03, 20 November 2017 (PST)&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
As mentioned on my electronic notebook, [[Bhamilton18 Week 12]], this week&amp;#039;s assignment Zachary Van Ysseldyk and I worked on the Coder Milestones 0-4. Zach and I will be working on the integration of the website bringing together the other groups work to function on the GRNsight website. Katie and Emma worked on their respective journal club presentation, but we communicated to check base on how everyone is doing in the progression of the project. Zach and I met in person to collaborate on formatting of the team page, individual journal pages and project progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; Zach and I worked on better time management as well as expectations for the assignment. We were able to communicate without any hiccups, and meeting up in person was ideal for clarifying issues.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; We were unsure of our progress in the project as our understanding of milestone 4 meant we needed to wait this week on others. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Checking in with our project teams/coders will help us understand how they are doing, as well as how our own progression is lining up.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 19:15, 20 November 2017 (PST)&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
This week, Blair and I worked on the coder milestones. We will work on integrating the actual other team&amp;#039;s parts as they start to finish. We also collaborated with Emma and Katie to make sure that we were on the same page. &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 12]]&amp;lt;br&amp;gt;&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; we were better about our time management as well as we communicated well. We met up together periodically to make sure that we were on the same page.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; We were a little confused on how to go about the project for our milestone considering that it was more of a waiting game this week rather than actual integration. So, we didn&amp;#039;t really know how to quantify our progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What we will do to fix it:&amp;#039;&amp;#039;&amp;#039; We maybe should have collaborated with the other teams a little bit more to be able and gauge how their projects are coming along.&lt;br /&gt;
&amp;lt;!----&lt;br /&gt;
===Week 14===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
===Week 15===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
---&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Bibliography==&lt;br /&gt;
===Paper 1===&lt;br /&gt;
Homma, T., Iwahashi, H., &amp;amp; Komatsu, Y. (2003). Yeast gene expression during growth at low temperature. &amp;#039;&amp;#039;Cryobiology&amp;#039;&amp;#039;, 46(3), 230-237. &lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/12818212&lt;br /&gt;
* PubMed Central:  not available&lt;br /&gt;
* Publisher Full Text (HTML):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=12818212&lt;br /&gt;
* Publisher Full Text (PDF): https://electra.lmu.edu:3555/S0011224003000282/1-s2.0-S0011224003000282-main.pdf?_tid=4c9b7cc0-c775-11e7-af71-00000aab0f26&amp;amp;acdnat=1510469407_3e02bee4266fd6a211ea3f6e27b460cf &lt;br /&gt;
* Copyright: 2003 by Science Direct; article is not Open Access&lt;br /&gt;
* Publisher:  Elsevier Science&lt;br /&gt;
* Availability: online at www.sciencedirect.com&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 2===&lt;br /&gt;
Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., ... &amp;amp; Fujita, K. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. &amp;#039;&amp;#039;Extremophiles&amp;#039;&amp;#039;, 10(2), 117-128.&lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/?term=Genome-wide+expression+analysis+of+yeast+response+during+exposure+to+4+degrees+C&lt;br /&gt;
* PubMed Central: not available&lt;br /&gt;
* Publisher Full Text (HTML): https://electra.lmu.edu:2529/article/10.1007/s00792-005-0480-1&lt;br /&gt;
* Publisher Full Text (PDF):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=16254683&lt;br /&gt;
* Copyright: 2005 by Springer-Verlag; article is not Open Access&lt;br /&gt;
* Publisher:  Springer-Verlag&lt;br /&gt;
* Availability: online&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 3===&lt;br /&gt;
Becerra, M., Lombardia, L. J., González‐Siso, M. I., Rodríguez‐Belmonte, E., Hauser, N. C., &amp;amp; Cerdán, M. E. (2003). Genome‐wide analysis of the yeast transcriptome upon heat and cold shock. Comparative and functional genomics, 4(4), 366-375.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/pdf/CFG-04-366.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML):&amp;#039;&amp;#039;&amp;#039; unavailable &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF):&amp;#039;&amp;#039;&amp;#039; http://downloads.hindawi.com/journals/ijg/2003/175356.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright:&amp;#039;&amp;#039;&amp;#039; Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher:&amp;#039;&amp;#039;&amp;#039; Hindawi Publishing Corporation&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability:&amp;#039;&amp;#039;&amp;#039; Open access&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; no&lt;br /&gt;
&lt;br /&gt;
===Paper 4===&lt;br /&gt;
Sahara, T., Goda, T., &amp;amp; Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract: &amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pubmed/?term=Comprehensive+expression+analysis+of+time-dependent+genetic+responses+in+yeast+cells+to+low+temperature&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central: &amp;#039;&amp;#039;&amp;#039; not available &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.long&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.full.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright: &amp;#039;&amp;#039;&amp;#039; &amp;quot;Other parties are welcome to copy, distribute, transmit and adapt the work — at no cost and without permission — for noncommercial use as long as they attribute the work to the original source using the citation above.&amp;quot; from http://www.jbc.org/site/misc/Copyright_Permission.xhtml&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher: &amp;#039;&amp;#039;&amp;#039; American Society for Biochemistry and Molecular Biology&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability: &amp;#039;&amp;#039;&amp;#039;All articles from this journal are free 1 year after publishing.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; No.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Acknowledgments==&lt;br /&gt;
#Our group collaborates on this page each week filling out our respective team journal assignments and project updates.&lt;br /&gt;
#*WE have a group chat to communicate problems, questions and ideas for our weekly assignments/milestones.&lt;br /&gt;
#We utilized the Calendar outline from the [[JASPAR the Friendly Ghost]] page, but changed the milestone/tasks due.&lt;br /&gt;
&lt;br /&gt;
==References==&lt;br /&gt;
*Meyer, R. (2015, July 09). The Secret Startup That Saved the Worst Website in America. Retrieved November 7, 2017, from https://www.theatlantic.com/technology/archive/2015/07/the-secret-startup- saved-healthcare-gov-the-worst-website-in-america/397784/&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;br /&gt;
&lt;br /&gt;
{{GRNsight Gene Page Project Links}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5049</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5049"/>
				<updated>2017-11-21T07:46:04Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Results of this study in comparison to the results of previous studies */ added new section, personal ideas for future research&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===Link to Presentation PDF===&lt;br /&gt;
[[media:Genome-wide expression analysis of yeast response during exposure to 4 degrees C.pdf|Genome-wide expression analysis of yeast response during exposure to 4 degrees C]]&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
*Cold shock response in yeast has been researched quite a bit, but the authors claim that this work is the first to study the effects at 4&amp;amp;deg;C. The Authors believe that their work is the first to study cold-shock response in yeast at a temperature below 10&amp;amp;deg;C.  &lt;br /&gt;
*It is important to study organisms at low temperatures because many organisms are exposed to freezing or near-freezing temperatures in the natural world.  Some organisms (such as &amp;#039;&amp;#039;E. coli&amp;#039;&amp;#039;) are quite often exposed to these low temperatures during experimental preparation.  &lt;br /&gt;
*It is also important to study the differences between organisms that have never been exposed to cold temperatures and organisms that have been grown in these cold conditions.  This will allow us to determine if/how well yeast can adapt to the cold.&lt;br /&gt;
&lt;br /&gt;
====Methods and Materials====&lt;br /&gt;
=====Preparation of yeast and probes=====&lt;br /&gt;
*Strain S288c was grown in the following conditions:&lt;br /&gt;
**Grown at 25°C until mid-log phase &lt;br /&gt;
**Cooled to 4°C and harvested after 6, 12, 24 &amp;amp; 48 h&lt;br /&gt;
**2 Controls grown continuously in 4°C &amp;amp; 35°C environments.&lt;br /&gt;
*RNA was extracted by the hot-phenol method. &lt;br /&gt;
*mRNA was purified with an mRNA purification kit. &lt;br /&gt;
*The probes were prepared by mixing 1-2μg of mRNA with two primers and  a dNTP nucleotide mix.  &lt;br /&gt;
**Reverse transcribed cDNA&lt;br /&gt;
**Probes were purified using a G-50 column&lt;br /&gt;
&lt;br /&gt;
=====Experimental design of the microarray data=====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
*Growth in yeast cells slowed once they were moved from 25&amp;amp;deg;C to 4&amp;amp;deg;C.    &lt;br /&gt;
*Hierarchical cluster analysis can be used to determine which sets of data are most similar to each other.  The process begins by grouping the two most similar data sets together into a &amp;quot;cluster.&amp;quot; Data sets are then added one-by-one to the initial cluster in order of similarity, until all data is categorized into one cluster. &amp;#039;&amp;#039;This technique revealed which time-points have the greatest similarity in gene expression.&amp;#039;&amp;#039;&lt;br /&gt;
**Hours 12 and 24 have the most similar gene-expression profile, and therefore are clustered together first.  &lt;br /&gt;
**The next closest to this cluster is 48 hours, which can then be added to the 12/24h  cluster form another, bigger cluster.  &lt;br /&gt;
**Hour 6 is the next closest to this cluster. &lt;br /&gt;
**Then the expression profile of the yeast constantly exposed to 4&amp;amp;deg;C &lt;br /&gt;
**And finally, with the least similar gene expression, is the yeast constantly exposed to 35&amp;amp;deg;C.&lt;br /&gt;
*Up and down-regulation of genes occurred according to what functions were needed for cell viability&lt;br /&gt;
**Genes involved in cell rescue, defense and virulence, and energy and metabolism were up-regulated &lt;br /&gt;
**Genes involved in protein synthesis, binding functions, activity regulation and fate were down-regulated&lt;br /&gt;
*Transcription of some genes go into overdrive after cold shock, others don’t&lt;br /&gt;
**Cold-shock causes up-regulation of genes that lead to&lt;br /&gt;
*** Energy preservation and cold tolerance&lt;br /&gt;
*** Membrane maintenance and permeability increases&lt;br /&gt;
***Detoxification of active oxygen species&lt;br /&gt;
***Revitalization of enzyme activity&lt;br /&gt;
**Causes down-regulation of genes that lead to growth&lt;br /&gt;
*** Allows yeast to adapt to new environment&lt;br /&gt;
&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
The results of this study looked very &amp;#039;&amp;#039;&amp;#039;similar&amp;#039;&amp;#039;&amp;#039; to those of previous studies.  Here are a couple of examples:&lt;br /&gt;
*Genes encoding HSPs were induced by 4&amp;amp;deg;C exposure.  These genes were also observed to be induced at 0&amp;amp;deg;C during a 2004 study of near-freezing response by Kang et al.  &lt;br /&gt;
*Trehalose synthesis was also shown to be important at low temperatures during a 2004 study by Kandror et al.  &lt;br /&gt;
&lt;br /&gt;
There are also some instances where the results of this study are &amp;#039;&amp;#039;&amp;#039;different&amp;#039;&amp;#039;&amp;#039; from previous ones.  For example:&lt;br /&gt;
*TPS1 and TPS2 genes were found to have a smaller fold induction in this study compared to a previous study by Kandror et al in 2004. &lt;br /&gt;
**This discrepancy may be explained by use of different carbon sources in the two studies.&lt;br /&gt;
&lt;br /&gt;
====Emma and Katie&amp;#039;s personal ideas on further research====&lt;br /&gt;
*How different types of stresses lead to similar or different changes in gene expression (e.g. salinity, pressure, chemicals, etc)&lt;br /&gt;
*If there is significant change in gene expression depending on the temperature used for cold shock (ie cold shock at 2°C, 4°C and 6°C and 8°C).&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5047</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5047"/>
				<updated>2017-11-21T07:44:18Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Results */ fixed syntax error&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===Link to Presentation PDF===&lt;br /&gt;
[[media:Genome-wide expression analysis of yeast response during exposure to 4 degrees C.pdf|Genome-wide expression analysis of yeast response during exposure to 4 degrees C]]&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
*Cold shock response in yeast has been researched quite a bit, but the authors claim that this work is the first to study the effects at 4&amp;amp;deg;C. The Authors believe that their work is the first to study cold-shock response in yeast at a temperature below 10&amp;amp;deg;C.  &lt;br /&gt;
*It is important to study organisms at low temperatures because many organisms are exposed to freezing or near-freezing temperatures in the natural world.  Some organisms (such as &amp;#039;&amp;#039;E. coli&amp;#039;&amp;#039;) are quite often exposed to these low temperatures during experimental preparation.  &lt;br /&gt;
*It is also important to study the differences between organisms that have never been exposed to cold temperatures and organisms that have been grown in these cold conditions.  This will allow us to determine if/how well yeast can adapt to the cold.&lt;br /&gt;
&lt;br /&gt;
====Methods and Materials====&lt;br /&gt;
=====Preparation of yeast and probes=====&lt;br /&gt;
*Strain S288c was grown in the following conditions:&lt;br /&gt;
**Grown at 25°C until mid-log phase &lt;br /&gt;
**Cooled to 4°C and harvested after 6, 12, 24 &amp;amp; 48 h&lt;br /&gt;
**2 Controls grown continuously in 4°C &amp;amp; 35°C environments.&lt;br /&gt;
*RNA was extracted by the hot-phenol method. &lt;br /&gt;
*mRNA was purified with an mRNA purification kit. &lt;br /&gt;
*The probes were prepared by mixing 1-2μg of mRNA with two primers and  a dNTP nucleotide mix.  &lt;br /&gt;
**Reverse transcribed cDNA&lt;br /&gt;
**Probes were purified using a G-50 column&lt;br /&gt;
&lt;br /&gt;
=====Experimental design of the microarray data=====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
*Growth in yeast cells slowed once they were moved from 25&amp;amp;deg;C to 4&amp;amp;deg;C.    &lt;br /&gt;
*Hierarchical cluster analysis can be used to determine which sets of data are most similar to each other.  The process begins by grouping the two most similar data sets together into a &amp;quot;cluster.&amp;quot; Data sets are then added one-by-one to the initial cluster in order of similarity, until all data is categorized into one cluster. &amp;#039;&amp;#039;This technique revealed which time-points have the greatest similarity in gene expression.&amp;#039;&amp;#039;&lt;br /&gt;
**Hours 12 and 24 have the most similar gene-expression profile, and therefore are clustered together first.  &lt;br /&gt;
**The next closest to this cluster is 48 hours, which can then be added to the 12/24h  cluster form another, bigger cluster.  &lt;br /&gt;
**Hour 6 is the next closest to this cluster. &lt;br /&gt;
**Then the expression profile of the yeast constantly exposed to 4&amp;amp;deg;C &lt;br /&gt;
**And finally, with the least similar gene expression, is the yeast constantly exposed to 35&amp;amp;deg;C.&lt;br /&gt;
*Up and down-regulation of genes occurred according to what functions were needed for cell viability&lt;br /&gt;
**Genes involved in cell rescue, defense and virulence, and energy and metabolism were up-regulated &lt;br /&gt;
**Genes involved in protein synthesis, binding functions, activity regulation and fate were down-regulated&lt;br /&gt;
*Transcription of some genes go into overdrive after cold shock, others don’t&lt;br /&gt;
**Cold-shock causes up-regulation of genes that lead to&lt;br /&gt;
*** Energy preservation and cold tolerance&lt;br /&gt;
*** Membrane maintenance and permeability increases&lt;br /&gt;
***Detoxification of active oxygen species&lt;br /&gt;
***Revitalization of enzyme activity&lt;br /&gt;
**Causes down-regulation of genes that lead to growth&lt;br /&gt;
*** Allows yeast to adapt to new environment&lt;br /&gt;
&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
The results of this study looked very &amp;#039;&amp;#039;&amp;#039;similar&amp;#039;&amp;#039;&amp;#039; to those of previous studies.  Here are a couple of examples:&lt;br /&gt;
*Genes encoding HSPs were induced by 4&amp;amp;deg;C exposure.  These genes were also observed to be induced at 0&amp;amp;deg;C during a 2004 study of near-freezing response by Kang et al.  &lt;br /&gt;
*Trehalose synthesis was also shown to be important at low temperatures during a 2004 study by Kandror et al.  &lt;br /&gt;
&lt;br /&gt;
There are also some instances where the results of this study are &amp;#039;&amp;#039;&amp;#039;different&amp;#039;&amp;#039;&amp;#039; from previous ones.  For example:&lt;br /&gt;
*TPS1 and TPS2 genes were found to have a smaller fold induction in this study compared to a previous study by Kandror et al in 2004. &lt;br /&gt;
**This discrepancy may be explained by use of different carbon sources in the two studies.&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5045</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5045"/>
				<updated>2017-11-21T07:43:33Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Results */ added more results&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===Link to Presentation PDF===&lt;br /&gt;
[[media:Genome-wide expression analysis of yeast response during exposure to 4 degrees C.pdf|Genome-wide expression analysis of yeast response during exposure to 4 degrees C]]&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
*Cold shock response in yeast has been researched quite a bit, but the authors claim that this work is the first to study the effects at 4&amp;amp;deg;C. The Authors believe that their work is the first to study cold-shock response in yeast at a temperature below 10&amp;amp;deg;C.  &lt;br /&gt;
*It is important to study organisms at low temperatures because many organisms are exposed to freezing or near-freezing temperatures in the natural world.  Some organisms (such as &amp;#039;&amp;#039;E. coli&amp;#039;&amp;#039;) are quite often exposed to these low temperatures during experimental preparation.  &lt;br /&gt;
*It is also important to study the differences between organisms that have never been exposed to cold temperatures and organisms that have been grown in these cold conditions.  This will allow us to determine if/how well yeast can adapt to the cold.&lt;br /&gt;
&lt;br /&gt;
====Methods and Materials====&lt;br /&gt;
=====Preparation of yeast and probes=====&lt;br /&gt;
*Strain S288c was grown in the following conditions:&lt;br /&gt;
**Grown at 25°C until mid-log phase &lt;br /&gt;
**Cooled to 4°C and harvested after 6, 12, 24 &amp;amp; 48 h&lt;br /&gt;
**2 Controls grown continuously in 4°C &amp;amp; 35°C environments.&lt;br /&gt;
*RNA was extracted by the hot-phenol method. &lt;br /&gt;
*mRNA was purified with an mRNA purification kit. &lt;br /&gt;
*The probes were prepared by mixing 1-2μg of mRNA with two primers and  a dNTP nucleotide mix.  &lt;br /&gt;
**Reverse transcribed cDNA&lt;br /&gt;
**Probes were purified using a G-50 column&lt;br /&gt;
&lt;br /&gt;
=====Experimental design of the microarray data=====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
*Growth in yeast cells slowed once they were moved from 25&amp;amp;deg;C to 4&amp;amp;deg;C.    &lt;br /&gt;
*Hierarchical cluster analysis can be used to determine which sets of data are most similar to each other.  The process begins by grouping the two most similar data sets together into a &amp;quot;cluster.&amp;quot; Data sets are then added one-by-one to the initial cluster in order of similarity, until all data is categorized into one cluster. &amp;#039;&amp;#039;This technique revealed which time-points have the greatest similarity in gene expression.&amp;#039;&amp;#039;&lt;br /&gt;
**Hours 12 and 24 have the most similar gene-expression profile, and therefore are clustered together first.  &lt;br /&gt;
**The next closest to this cluster is 48 hours, which can then be added to the 12/24h  cluster form another, bigger cluster.  &lt;br /&gt;
**Hour 6 is the next closest to this cluster. &lt;br /&gt;
**Then the expression profile of the yeast constantly exposed to 4&amp;amp;deg;C &lt;br /&gt;
**And finally, with the least similar gene expression, is the yeast constantly exposed to 35&amp;amp;deg;C.&lt;br /&gt;
*Up and down-regulation of genes occurred according to what functions were needed for cell viability&lt;br /&gt;
**Genes involved in cell rescue, defense and virulence, and energy and metabolism were up-regulated &lt;br /&gt;
**Genes involved in protein synthesis, binding functions, activity regulation and fate were down-regulated&lt;br /&gt;
*Transcription of some genes go into&lt;br /&gt;
overdrive after cold shock, others don’t&lt;br /&gt;
** Cold-shock causes up-regulation of genes that lead to&lt;br /&gt;
*** Energy preservation and cold tolerance&lt;br /&gt;
*** Membrane maintenance and permeability increases&lt;br /&gt;
***Detoxification of active oxygen species&lt;br /&gt;
***Revitalization of enzyme activity&lt;br /&gt;
**Causes down-regulation of genes that lead to growth&lt;br /&gt;
*** Allows yeast to adapt to new environment&lt;br /&gt;
&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
The results of this study looked very &amp;#039;&amp;#039;&amp;#039;similar&amp;#039;&amp;#039;&amp;#039; to those of previous studies.  Here are a couple of examples:&lt;br /&gt;
*Genes encoding HSPs were induced by 4&amp;amp;deg;C exposure.  These genes were also observed to be induced at 0&amp;amp;deg;C during a 2004 study of near-freezing response by Kang et al.  &lt;br /&gt;
*Trehalose synthesis was also shown to be important at low temperatures during a 2004 study by Kandror et al.  &lt;br /&gt;
&lt;br /&gt;
There are also some instances where the results of this study are &amp;#039;&amp;#039;&amp;#039;different&amp;#039;&amp;#039;&amp;#039; from previous ones.  For example:&lt;br /&gt;
*TPS1 and TPS2 genes were found to have a smaller fold induction in this study compared to a previous study by Kandror et al in 2004. &lt;br /&gt;
**This discrepancy may be explained by use of different carbon sources in the two studies.&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5038</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5038"/>
				<updated>2017-11-21T07:40:28Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: added link to presentation&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===Link to Presentation PDF===&lt;br /&gt;
[[media:Genome-wide expression analysis of yeast response during exposure to 4 degrees C.pdf|Genome-wide expression analysis of yeast response during exposure to 4 degrees C]]&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
*Cold shock response in yeast has been researched quite a bit, but the authors claim that this work is the first to study the effects at 4&amp;amp;deg;C. The Authors believe that their work is the first to study cold-shock response in yeast at a temperature below 10&amp;amp;deg;C.  &lt;br /&gt;
*It is important to study organisms at low temperatures because many organisms are exposed to freezing or near-freezing temperatures in the natural world.  Some organisms (such as &amp;#039;&amp;#039;E. coli&amp;#039;&amp;#039;) are quite often exposed to these low temperatures during experimental preparation.  &lt;br /&gt;
*It is also important to study the differences between organisms that have never been exposed to cold temperatures and organisms that have been grown in these cold conditions.  This will allow us to determine if/how well yeast can adapt to the cold.&lt;br /&gt;
&lt;br /&gt;
====Methods and Materials====&lt;br /&gt;
=====Preparation of yeast and probes=====&lt;br /&gt;
*Strain S288c was grown in the following conditions:&lt;br /&gt;
**Grown at 25°C until mid-log phase &lt;br /&gt;
**Cooled to 4°C and harvested after 6, 12, 24 &amp;amp; 48 h&lt;br /&gt;
**2 Controls grown continuously in 4°C &amp;amp; 35°C environments.&lt;br /&gt;
*RNA was extracted by the hot-phenol method. &lt;br /&gt;
*mRNA was purified with an mRNA purification kit. &lt;br /&gt;
*The probes were prepared by mixing 1-2μg of mRNA with two primers and  a dNTP nucleotide mix.  &lt;br /&gt;
**Reverse transcribed cDNA&lt;br /&gt;
**Probes were purified using a G-50 column&lt;br /&gt;
&lt;br /&gt;
=====Experimental design of the microarray data=====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
*Growth in yeast cells slowed once they were moved from 25&amp;amp;deg;C to 4&amp;amp;deg;C.    &lt;br /&gt;
*Hierarchical cluster analysis can be used to determine which sets of data are most similar to each other.  The process begins by grouping the two most similar data sets together into a &amp;quot;cluster.&amp;quot; Data sets are then added one-by-one to the initial cluster in order of similarity, until all data is categorized into one cluster. &amp;#039;&amp;#039;This technique revealed which time-points have the greatest similarity in gene expression.&amp;#039;&amp;#039;&lt;br /&gt;
**Hours 12 and 24 have the most similar gene-expression profile, and therefore are clustered together first.  &lt;br /&gt;
**The next closest to this cluster is 48 hours, which can then be added to the 12/24h  cluster form another, bigger cluster.  &lt;br /&gt;
**Hour 6 is the next closest to this cluster. &lt;br /&gt;
**Then the expression profile of the yeast constantly exposed to 4&amp;amp;deg;C &lt;br /&gt;
**And finally, with the least similar gene expression, is the yeast constantly exposed to 35&amp;amp;deg;C.&lt;br /&gt;
&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
The results of this study looked very &amp;#039;&amp;#039;&amp;#039;similar&amp;#039;&amp;#039;&amp;#039; to those of previous studies.  Here are a couple of examples:&lt;br /&gt;
*Genes encoding HSPs were induced by 4&amp;amp;deg;C exposure.  These genes were also observed to be induced at 0&amp;amp;deg;C during a 2004 study of near-freezing response by Kang et al.  &lt;br /&gt;
*Trehalose synthesis was also shown to be important at low temperatures during a 2004 study by Kandror et al.  &lt;br /&gt;
&lt;br /&gt;
There are also some instances where the results of this study are &amp;#039;&amp;#039;&amp;#039;different&amp;#039;&amp;#039;&amp;#039; from previous ones.  For example:&lt;br /&gt;
*TPS1 and TPS2 genes were found to have a smaller fold induction in this study compared to a previous study by Kandror et al in 2004. &lt;br /&gt;
**This discrepancy may be explained by use of different carbon sources in the two studies.&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=5030</id>
		<title>Lights, Camera, InterACTION!</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=5030"/>
				<updated>2017-11-21T07:33:02Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* File Roundup */ added link to Katie and Emma&amp;#039;s week 12 presentation&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:PugNotDrugsLogo.png|400px|thumb|right|Pugs Not Drugs Logo]]&lt;br /&gt;
&lt;br /&gt;
==Team Members==&lt;br /&gt;
*[[user:bhamilton18|Blair Hamilton]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:emmatyrnauer|Emma Tyrnauer]] &amp;#039;&amp;#039;&amp;#039;-Data Analyst&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:kwrigh35|Katie Wright]] &amp;#039;&amp;#039;&amp;#039;-Project Manager/Quality Assurance&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:zvanysse|Zach Van Ysseldyke]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
==File Roundup==&lt;br /&gt;
*Week 11 presentation by Blair and Zach: [[media:The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf|The Secret Startup that Saved the Worst Website in America]]&lt;br /&gt;
*Week 12 presentation by Emma and Katie: [[media:Genome-wide expression analysis of yeast response during exposure to 4 degrees C.pdf|Genome-wide expression analysis of yeast response during exposure to 4 degrees C]]&lt;br /&gt;
&lt;br /&gt;
==Team Schedule== &amp;lt;!--Borrowed Format from JASPAR group--&amp;gt;&lt;br /&gt;
{| class =&amp;quot;wikitable&amp;quot;&lt;br /&gt;
|+November &amp;amp; December&lt;br /&gt;
|-&lt;br /&gt;
|Sunday&lt;br /&gt;
|Monday&lt;br /&gt;
|Tuesday&lt;br /&gt;
|Wednesday&lt;br /&gt;
|Thursday&lt;br /&gt;
|Friday&lt;br /&gt;
|Saturday&lt;br /&gt;
|-&lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt; C: PPT Presentation&amp;lt;br&amp;gt;PM/DA: Journal Articles&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;17&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;18&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;19&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;20&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;21&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;C: Complete setup steps (Milestones 0-4)&amp;lt;br&amp;gt;PM/DA: Journal Club Presentations&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;22&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;23&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;24&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;25&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;26&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;27&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;28&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: First Integration projected completion&amp;lt;br&amp;gt;PM: &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;29&amp;#039;&amp;#039;&amp;#039; &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;30&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM: &amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with groups, and further integrate as needed.&amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM: &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;Final group Presentation &amp;#039;&amp;#039;2:00-4:00PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;All deliverables due &amp;#039;&amp;#039;4:30PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Executive Summaries==&lt;br /&gt;
===Week 11===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
For this week, I worked with [[user:emmatyrnauer|Emma]] to find papers to use as reference in our final research paper.  Emma and I both found papers that are related to yeast cold shock reactions and most of them use microarray data.  I am excited to read these articles more in depth as we develop our research project and paper.  Further information can be found on my [[kwrigh35 Week 11|Week 11]] journal page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039; Using the three different sources was really helpful because I had never used &amp;quot;Web of Science&amp;quot; before.  It was good to learn about how different these results can be, especially with tweaking the search terms a little bit.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I had poor time management this week.  I waited until Monday to do the majority of the work and definitely should not have done so.  The rest of the project went well, however.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I will plan to meet up with Emma early on in the week so we can work together on our presentation.  This way I will be held accountable to get my work done earlier.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week Katie and I worked on the annotated bibliography (below). We chose papers that provided more information on yeast gene regulation in response to cold shock. Pub Med, Google Scholar, and Web of Science were utilized to perform basic searches using key words as well as advanced searches which helped to narrow the results. More detailed information can be found on my individual journal entry page: [[Emmatyrnauer Week 11]]. Meanwhile, Blair and Zach worked on coder journal club presentation. Construction of the group page was completed during class.&lt;br /&gt;
# Overall, this week went pretty smoothly; Katie and I communicated over text to determine which papers we were going to write the bibliographies for and were available to each other for any questions we had.&lt;br /&gt;
# There wasn&amp;#039;t really anything that didn&amp;#039;t work.&lt;br /&gt;
# Since we will be working this next week on our journal club presentation, it might be beneficial to meet in person.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:26, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
This week Zach and I created the Powerpoint presentation about &amp;#039;&amp;#039;The secret startup that saved the worst website in America&amp;#039;&amp;#039; article. We created individual outlines of the article on our respective pages, mine is as follows: [[Bhamilton18 Week 11]]. Our presentation is linked here: [[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]]&lt;br /&gt;
#Overall, our communication and diving up of tasks went well and smoothly. Meanwhile, Katie and Emma worked on the annotate bibliography. While I primarily worked with Zach our communication amongst group members went well and we hope to continue to do so for the future.&lt;br /&gt;
#Something that didn&amp;#039;t work as well was our time management. Next time we hope to have more class time and start working earlier on our portion of the assignment. &lt;br /&gt;
#For the future, we will continue to communicate in person and through messaging and will work on time management by starting earlier.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 23:35, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
[[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]] &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 11]]&amp;lt;br&amp;gt;&lt;br /&gt;
This week, Blair and I created a Powerpoint about the &amp;quot;Secret Startup that saved the worst website in America.&amp;quot; We read through the article and picked out the relevant themes and main messages to put on the PowerPoint. We wrote these themes and summaries out on our individual Journals. Then, we collaborated and made our PowerPoint. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; Blair and I&amp;#039;s communication was very strong and we knew exactly what was going on with one another. We knew when we were going to meet up and what was expected for each of us.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Blair and I missed the mark on our time management. We did not expect this assignment to take this long. Next time, we will make sure to start on assignments earlier.&lt;br /&gt;
#Like I said above, we will plan out exact times earlier on in the week that we can meet up&lt;br /&gt;
[[User:Zvanysse|Zvanysse]] ([[User talk:Zvanysse|talk]]) 23:44, 13 November 2017 (PST) &amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
===Week 12===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
Emma and I worked on our presentation on &amp;quot;Genome-wide expression analysis of yeast response during exposure to 4 degrees C,&amp;quot; details of which can be found on my [[kwrigh35 Week 12|Week 12]] page.  The presentation can be found in the &amp;quot;file roundup&amp;quot; section of this page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039;Emma and I met up outside of class to work on the assignment, which was really helpful.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I underestimated to the time it would take to find 10 terms that I didn&amp;#039;t know before reading the paper.  It was hard to find 10, so I resorted to picking terms that I knew but wanted to know more about.  It was also difficult to find definitions for some of these words, because some of them weren&amp;#039;t in biological dictionaries.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I should start work on the assignment before the weekend begins.  That way, I don&amp;#039;t have to spend the majority of my Monday afternoon/evening on the project.  It is better to be able to come back to a project several times, and each time have &amp;quot;fresh&amp;quot; eyes.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week I made all the corrections listed on the talk page of my user page for both [[Emmatyrnauer Week 8]] and [[Emmatyrnauer Week 10]]. This included corrections to the excel files, stem analysis, electronic notebooks, analysis of log fold changes and p values, and the acknowledgements section. Following the completion of fixing the errors in the excel file, I emailed Dr. Dahlquist to ascertain that I completed the analysis correctly. For the individual assignment this week, Katie and I met up to work on our journal club presentation of the paper that we were assigned (can be accessed on my individual journal page, [[Emmatyrnauer Week 12]]. We divided up what portions we would be responsible for and have planned to meet up before class tomorrow to practice.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; It was very helpful to meet up in person with Katie to begin working on the powerpoint before separating everything out.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; I could have been in better communication with Blair and Zach this week.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Sitting down together as a group will help to get us all on the same page for the future weeks.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:03, 20 November 2017 (PST)&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
As mentioned on my electronic notebook, [[Bhamilton18 Week 12]], this week&amp;#039;s assignment Zachary Van Ysseldyk and I worked on the Coder Milestones 0-4. Zach and I will be working on the integration of the website bringing together the other groups work to function on the GRNsight website. Katie and Emma worked on their respective journal club presentation, but we communicated to check base on how everyone is doing in the progression of the project. Zach and I met in person to collaborate on formatting of the team page, individual journal pages and project progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; Zach and I worked on better time management as well as expectations for the assignment. We were able to communicate without any hiccups, and meeting up in person was ideal for clarifying issues.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; We were unsure of our progress in the project as our understanding of milestone 4 meant we needed to wait this week on others. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Checking in with our project teams/coders will help us understand how they are doing, as well as how our own progression is lining up.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 19:15, 20 November 2017 (PST)&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
This week, Blair and I worked on the coder milestones. We will work on integrating the actual other team&amp;#039;s parts as they start to finish. We also collaborated with Emma and Katie to make sure that we were on the same page. &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 12]]&amp;lt;br&amp;gt;&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; we were better about our time management as well as we communicated well. We met up together periodically to make sure that we were on the same page.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; We were a little confused on how to go about the project for our milestone considering that it was more of a waiting game this week rather than actual integration. So, we didn&amp;#039;t really know how to quantify our progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What we will do to fix it:&amp;#039;&amp;#039;&amp;#039; We maybe should have collaborated with the other teams a little bit more to be able and gauge how their projects are coming along.&lt;br /&gt;
&amp;lt;!----&lt;br /&gt;
===Week 14===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
===Week 15===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
---&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Bibliography==&lt;br /&gt;
===Paper 1===&lt;br /&gt;
Homma, T., Iwahashi, H., &amp;amp; Komatsu, Y. (2003). Yeast gene expression during growth at low temperature. &amp;#039;&amp;#039;Cryobiology&amp;#039;&amp;#039;, 46(3), 230-237. &lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/12818212&lt;br /&gt;
* PubMed Central:  not available&lt;br /&gt;
* Publisher Full Text (HTML):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=12818212&lt;br /&gt;
* Publisher Full Text (PDF): https://electra.lmu.edu:3555/S0011224003000282/1-s2.0-S0011224003000282-main.pdf?_tid=4c9b7cc0-c775-11e7-af71-00000aab0f26&amp;amp;acdnat=1510469407_3e02bee4266fd6a211ea3f6e27b460cf &lt;br /&gt;
* Copyright: 2003 by Science Direct; article is not Open Access&lt;br /&gt;
* Publisher:  Elsevier Science&lt;br /&gt;
* Availability: online at www.sciencedirect.com&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 2===&lt;br /&gt;
Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., ... &amp;amp; Fujita, K. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. &amp;#039;&amp;#039;Extremophiles&amp;#039;&amp;#039;, 10(2), 117-128.&lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/?term=Genome-wide+expression+analysis+of+yeast+response+during+exposure+to+4+degrees+C&lt;br /&gt;
* PubMed Central: not available&lt;br /&gt;
* Publisher Full Text (HTML): https://electra.lmu.edu:2529/article/10.1007/s00792-005-0480-1&lt;br /&gt;
* Publisher Full Text (PDF):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=16254683&lt;br /&gt;
* Copyright: 2005 by Springer-Verlag; article is not Open Access&lt;br /&gt;
* Publisher:  Springer-Verlag&lt;br /&gt;
* Availability: online&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 3===&lt;br /&gt;
Becerra, M., Lombardia, L. J., González‐Siso, M. I., Rodríguez‐Belmonte, E., Hauser, N. C., &amp;amp; Cerdán, M. E. (2003). Genome‐wide analysis of the yeast transcriptome upon heat and cold shock. Comparative and functional genomics, 4(4), 366-375.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/pdf/CFG-04-366.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML):&amp;#039;&amp;#039;&amp;#039; unavailable &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF):&amp;#039;&amp;#039;&amp;#039; http://downloads.hindawi.com/journals/ijg/2003/175356.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright:&amp;#039;&amp;#039;&amp;#039; Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher:&amp;#039;&amp;#039;&amp;#039; Hindawi Publishing Corporation&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability:&amp;#039;&amp;#039;&amp;#039; Open access&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; no&lt;br /&gt;
&lt;br /&gt;
===Paper 4===&lt;br /&gt;
Sahara, T., Goda, T., &amp;amp; Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract: &amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pubmed/?term=Comprehensive+expression+analysis+of+time-dependent+genetic+responses+in+yeast+cells+to+low+temperature&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central: &amp;#039;&amp;#039;&amp;#039; not available &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.long&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.full.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright: &amp;#039;&amp;#039;&amp;#039; &amp;quot;Other parties are welcome to copy, distribute, transmit and adapt the work — at no cost and without permission — for noncommercial use as long as they attribute the work to the original source using the citation above.&amp;quot; from http://www.jbc.org/site/misc/Copyright_Permission.xhtml&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher: &amp;#039;&amp;#039;&amp;#039; American Society for Biochemistry and Molecular Biology&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability: &amp;#039;&amp;#039;&amp;#039;All articles from this journal are free 1 year after publishing.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; No.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Acknowledgments==&lt;br /&gt;
#Our group collaborates on this page each week filling out our respective team journal assignments and project updates.&lt;br /&gt;
#*WE have a group chat to communicate problems, questions and ideas for our weekly assignments/milestones.&lt;br /&gt;
#We utilized the Calendar outline from the [[JASPAR the Friendly Ghost]] page, but changed the milestone/tasks due.&lt;br /&gt;
&lt;br /&gt;
==References==&lt;br /&gt;
*Meyer, R. (2015, July 09). The Secret Startup That Saved the Worst Website in America. Retrieved November 7, 2017, from https://www.theatlantic.com/technology/archive/2015/07/the-secret-startup- saved-healthcare-gov-the-worst-website-in-america/397784/&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;br /&gt;
&lt;br /&gt;
{{GRNsight Gene Page Project Links}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=File:Genome-wide_expression_analysis_of_yeast_response_during_exposure_to_4_degrees_C.pdf&amp;diff=5029</id>
		<title>File:Genome-wide expression analysis of yeast response during exposure to 4 degrees C.pdf</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=File:Genome-wide_expression_analysis_of_yeast_response_during_exposure_to_4_degrees_C.pdf&amp;diff=5029"/>
				<updated>2017-11-21T07:31:23Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: Emma and Katie&amp;#039;s week 12 presentation.  Part of team Lights, Camera, InterACTION!&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;Emma and Katie&amp;#039;s week 12 presentation.  Part of team Lights, Camera, InterACTION!&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5021</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5021"/>
				<updated>2017-11-21T07:22:33Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Article Outline */ added section on materials and methods, changed heading level of Microarray design, added info on preparation of yeast and probes&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
*Cold shock response in yeast has been researched quite a bit, but the authors claim that this work is the first to study the effects at 4&amp;amp;deg;C. The Authors believe that their work is the first to study cold-shock response in yeast at a temperature below 10&amp;amp;deg;C.  &lt;br /&gt;
*It is important to study organisms at low temperatures because many organisms are exposed to freezing or near-freezing temperatures in the natural world.  Some organisms (such as &amp;#039;&amp;#039;E. coli&amp;#039;&amp;#039;) are quite often exposed to these low temperatures during experimental preparation.  &lt;br /&gt;
*It is also important to study the differences between organisms that have never been exposed to cold temperatures and organisms that have been grown in these cold conditions.  This will allow us to determine if/how well yeast can adapt to the cold.&lt;br /&gt;
&lt;br /&gt;
====Methods and Materials====&lt;br /&gt;
=====Preparation of yeast and probes=====&lt;br /&gt;
*Strain S288c was grown in the following conditions:&lt;br /&gt;
**Grown at 25°C until mid-log phase &lt;br /&gt;
**Cooled to 4°C and harvested after 6, 12, 24 &amp;amp; 48 h&lt;br /&gt;
**2 Controls grown continuously in 4°C &amp;amp; 35°C environments.&lt;br /&gt;
*RNA was extracted by the hot-phenol method. &lt;br /&gt;
*mRNA was purified with an mRNA purification kit. &lt;br /&gt;
*The probes were prepared by mixing 1-2μg of mRNA with two primers and  a dNTP nucleotide mix.  &lt;br /&gt;
**Reverse transcribed cDNA&lt;br /&gt;
**Probes were purified using a G-50 column&lt;br /&gt;
&lt;br /&gt;
=====Experimental design of the microarray data=====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
*Growth in yeast cells slowed once they were moved from 25&amp;amp;deg;C to 4&amp;amp;deg;C.    &lt;br /&gt;
*Hierarchical cluster analysis can be used to determine which sets of data are most similar to each other.  The process begins by grouping the two most similar data sets together into a &amp;quot;cluster.&amp;quot; Data sets are then added one-by-one to the initial cluster in order of similarity, until all data is categorized into one cluster. &amp;#039;&amp;#039;This technique revealed which time-points have the greatest similarity in gene expression.&amp;#039;&amp;#039;&lt;br /&gt;
**Hours 12 and 24 have the most similar gene-expression profile, and therefore are clustered together first.  &lt;br /&gt;
**The next closest to this cluster is 48 hours, which can then be added to the 12/24h  cluster form another, bigger cluster.  &lt;br /&gt;
**Hour 6 is the next closest to this cluster. &lt;br /&gt;
**Then the expression profile of the yeast constantly exposed to 4&amp;amp;deg;C &lt;br /&gt;
**And finally, with the least similar gene expression, is the yeast constantly exposed to 35&amp;amp;deg;C.&lt;br /&gt;
&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
The results of this study looked very &amp;#039;&amp;#039;&amp;#039;similar&amp;#039;&amp;#039;&amp;#039; to those of previous studies.  Here are a couple of examples:&lt;br /&gt;
*Genes encoding HSPs were induced by 4&amp;amp;deg;C exposure.  These genes were also observed to be induced at 0&amp;amp;deg;C during a 2004 study of near-freezing response by Kang et al.  &lt;br /&gt;
*Trehalose synthesis was also shown to be important at low temperatures during a 2004 study by Kandror et al.  &lt;br /&gt;
&lt;br /&gt;
There are also some instances where the results of this study are &amp;#039;&amp;#039;&amp;#039;different&amp;#039;&amp;#039;&amp;#039; from previous ones.  For example:&lt;br /&gt;
*TPS1 and TPS2 genes were found to have a smaller fold induction in this study compared to a previous study by Kandror et al in 2004. &lt;br /&gt;
**This discrepancy may be explained by use of different carbon sources in the two studies.&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5000</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=5000"/>
				<updated>2017-11-21T06:51:59Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Results of this study in comparison to the results of previous studies */ added a response&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
*Cold shock response in yeast has been researched quite a bit, but the authors claim that this work is the first to study the effects at 4&amp;amp;deg;C. The Authors believe that their work is the first to study cold-shock response in yeast at a temperature below 10&amp;amp;deg;C.  &lt;br /&gt;
*It is important to study organisms at low temperatures because many organisms are exposed to freezing or near-freezing temperatures in the natural world.  Some organisms (such as &amp;#039;&amp;#039;E. coli&amp;#039;&amp;#039;) are quite often exposed to these low temperatures during experimental preparation.  &lt;br /&gt;
*It is also important to study the differences between organisms that have never been exposed to cold temperatures and organisms that have been grown in these cold conditions.  This will allow us to determine if/how well yeast can adapt to the cold.&lt;br /&gt;
&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
*Growth in yeast cells slowed once they were moved from 25&amp;amp;deg;C to 4&amp;amp;deg;C.    &lt;br /&gt;
*Hierarchical cluster analysis can be used to determine which sets of data are most similar to each other.  The process begins by grouping the two most similar data sets together into a &amp;quot;cluster.&amp;quot; Data sets are then added one-by-one to the initial cluster in order of similarity, until all data is categorized into one cluster. &amp;#039;&amp;#039;This technique revealed which time-points have the greatest similarity in gene expression.&amp;#039;&amp;#039;&lt;br /&gt;
**Hours 12 and 24 have the most similar gene-expression profile, and therefore are clustered together first.  &lt;br /&gt;
**The next closest to this cluster is 48 hours, which can then be added to the 12/24h  cluster form another, bigger cluster.  &lt;br /&gt;
**Hour 6 is the next closest to this cluster. &lt;br /&gt;
**Then the expression profile of the yeast constantly exposed to 4&amp;amp;deg;C &lt;br /&gt;
**And finally, with the least similar gene expression, is the yeast constantly exposed to 35&amp;amp;deg;C.&lt;br /&gt;
&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
The results of this study looked very &amp;#039;&amp;#039;&amp;#039;similar&amp;#039;&amp;#039;&amp;#039; to those of previous studies.  Here are a couple of examples:&lt;br /&gt;
*Genes encoding HSPs were induced by 4&amp;amp;deg;C exposure.  These genes were also observed to be induced at 0&amp;amp;deg;C during a 2004 study of near-freezing response by Kang et al.  &lt;br /&gt;
*Trehalose synthesis was also shown to be important at low temperatures during a 2004 study by Kandror et al.  &lt;br /&gt;
&lt;br /&gt;
There are also some instances where the results of this study are &amp;#039;&amp;#039;&amp;#039;different&amp;#039;&amp;#039;&amp;#039; from previous ones.  For example:&lt;br /&gt;
*TPS1 and TPS2 genes were found to have a smaller fold induction in this study compared to a previous study by Kandror et al in 2004. &lt;br /&gt;
**This discrepancy may be explained by use of different carbon sources in the two studies.&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=4987</id>
		<title>Lights, Camera, InterACTION!</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=4987"/>
				<updated>2017-11-21T06:35:44Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Katie - PM/QA */ added response.&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:PugNotDrugsLogo.png|400px|thumb|right|Pugs Not Drugs Logo]]&lt;br /&gt;
&lt;br /&gt;
==Team Members==&lt;br /&gt;
*[[user:bhamilton18|Blair Hamilton]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:emmatyrnauer|Emma Tyrnauer]] &amp;#039;&amp;#039;&amp;#039;-Data Analyst&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:kwrigh35|Katie Wright]] &amp;#039;&amp;#039;&amp;#039;-Project Manager/Quality Assurance&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:zvanysse|Zach Van Ysseldyke]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
==File Roundup==&lt;br /&gt;
*Week 11 presentation by Blair and Zach: [[media:The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf|The Secret Startup that Saved the Worst Website in America]]&lt;br /&gt;
&lt;br /&gt;
==Team Schedule== &amp;lt;!--Borrowed Format from JASPAR group--&amp;gt;&lt;br /&gt;
{| class =&amp;quot;wikitable&amp;quot;&lt;br /&gt;
|+November &amp;amp; December&lt;br /&gt;
|-&lt;br /&gt;
|Sunday&lt;br /&gt;
|Monday&lt;br /&gt;
|Tuesday&lt;br /&gt;
|Wednesday&lt;br /&gt;
|Thursday&lt;br /&gt;
|Friday&lt;br /&gt;
|Saturday&lt;br /&gt;
|-&lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt; C: PPT Presentation&amp;lt;br&amp;gt;PM/DA: Journal Articles&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;17&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;18&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;19&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;20&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;21&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;C: Complete setup steps (Milestones 0-4)&amp;lt;br&amp;gt;PM/DA: Journal Club Presentations&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;22&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;23&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;24&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;25&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;26&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;27&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;28&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: First Integration projected completion&amp;lt;br&amp;gt;PM: &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;29&amp;#039;&amp;#039;&amp;#039; &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;30&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM: &amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with groups, and further integrate as needed.&amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM: &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;Final group Presentation &amp;#039;&amp;#039;2:00-4:00PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;All deliverables due &amp;#039;&amp;#039;4:30PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Executive Summaries==&lt;br /&gt;
===Week 11===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
For this week, I worked with [[user:emmatyrnauer|Emma]] to find papers to use as reference in our final research paper.  Emma and I both found papers that are related to yeast cold shock reactions and most of them use microarray data.  I am excited to read these articles more in depth as we develop our research project and paper.  Further information can be found on my [[kwrigh35 Week 11|Week 11]] journal page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039; Using the three different sources was really helpful because I had never used &amp;quot;Web of Science&amp;quot; before.  It was good to learn about how different these results can be, especially with tweaking the search terms a little bit.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I had poor time management this week.  I waited until Monday to do the majority of the work and definitely should not have done so.  The rest of the project went well, however.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I will plan to meet up with Emma early on in the week so we can work together on our presentation.  This way I will be held accountable to get my work done earlier.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week Katie and I worked on the annotated bibliography (below). We chose papers that provided more information on yeast gene regulation in response to cold shock. Pub Med, Google Scholar, and Web of Science were utilized to perform basic searches using key words as well as advanced searches which helped to narrow the results. More detailed information can be found on my individual journal entry page: [[Emmatyrnauer Week 11]]. Meanwhile, Blair and Zach worked on coder journal club presentation. Construction of the group page was completed during class.&lt;br /&gt;
# Overall, this week went pretty smoothly; Katie and I communicated over text to determine which papers we were going to write the bibliographies for and were available to each other for any questions we had.&lt;br /&gt;
# There wasn&amp;#039;t really anything that didn&amp;#039;t work.&lt;br /&gt;
# Since we will be working this next week on our journal club presentation, it might be beneficial to meet in person.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:26, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
This week Zach and I created the Powerpoint presentation about &amp;#039;&amp;#039;The secret startup that saved the worst website in America&amp;#039;&amp;#039; article. We created individual outlines of the article on our respective pages, mine is as follows: [[Bhamilton18 Week 11]]. Our presentation is linked here: [[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]]&lt;br /&gt;
#Overall, our communication and diving up of tasks went well and smoothly. Meanwhile, Katie and Emma worked on the annotate bibliography. While I primarily worked with Zach our communication amongst group members went well and we hope to continue to do so for the future.&lt;br /&gt;
#Something that didn&amp;#039;t work as well was our time management. Next time we hope to have more class time and start working earlier on our portion of the assignment. &lt;br /&gt;
#For the future, we will continue to communicate in person and through messaging and will work on time management by starting earlier.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 23:35, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
[[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]] &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 11]]&amp;lt;br&amp;gt;&lt;br /&gt;
This week, Blair and I created a Powerpoint about the &amp;quot;Secret Startup that saved the worst website in America.&amp;quot; We read through the article and picked out the relevant themes and main messages to put on the PowerPoint. We wrote these themes and summaries out on our individual Journals. Then, we collaborated and made our PowerPoint. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; Blair and I&amp;#039;s communication was very strong and we knew exactly what was going on with one another. We knew when we were going to meet up and what was expected for each of us.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Blair and I missed the mark on our time management. We did not expect this assignment to take this long. Next time, we will make sure to start on assignments earlier.&lt;br /&gt;
#Like I said above, we will plan out exact times earlier on in the week that we can meet up&lt;br /&gt;
[[User:Zvanysse|Zvanysse]] ([[User talk:Zvanysse|talk]]) 23:44, 13 November 2017 (PST) &amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
===Week 12===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
Emma and I worked on our presentation on &amp;quot;Genome-wide expression analysis of yeast response during exposure to 4 degrees C,&amp;quot; details of which can be found on my [[kwrigh35 Week 12|Week 12]] page.  The presentation can be found in the &amp;quot;file roundup&amp;quot; section of this page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039;Emma and I met up outside of class to work on the assignment, which was really helpful.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I underestimated to the time it would take to find 10 terms that I didn&amp;#039;t know before reading the paper.  It was hard to find 10, so I resorted to picking terms that I knew but wanted to know more about.  It was also difficult to find definitions for some of these words, because some of them weren&amp;#039;t in biological dictionaries.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039;I should start work on the assignment before the weekend begins.  That way, I don&amp;#039;t have to spend the majority of my Monday afternoon/evening on the project.  It is better to be able to come back to a project several times, and each time have &amp;quot;fresh&amp;quot; eyes.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week I made all the corrections listed on the talk page of my user page for both [[Emmatyrnauer Week 8]] and [[Emmatyrnauer Week 10]]. This included corrections to the excel files, stem analysis, electronic notebooks, analysis of log fold changes and p values, and the acknowledgements section. Following the completion of fixing the errors in the excel file, I emailed Dr. Dahlquist to ascertain that I completed the analysis correctly. For the individual assignment this week, Katie and I met up to work on our journal club presentation of the paper that we were assigned (can be accessed on my individual journal page, [[Emmatyrnauer Week 12]]. We divided up what portions we would be responsible for and have planned to meet up before class tomorrow to practice.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; It was very helpful to meet up in person with Katie to begin working on the powerpoint before separating everything out.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; I could have been in better communication with Blair and Zach this week.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Sitting down together as a group will help to get us all on the same page for the future weeks.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:03, 20 November 2017 (PST)&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
As mentioned on my electronic notebook, [[Bhamilton18 Week 12]], this week&amp;#039;s assignment Zachary Van Ysseldyk and I worked on the Coder Milestones 0-4. Zach and I will be working on the integration of the website bringing together the other groups work to function on the GRNsight website. Katie and Emma worked on their respective journal club presentation, but we communicated to check base on how everyone is doing in the progression of the project. Zach and I met in person to collaborate on formatting of the team page, individual journal pages and project progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; Zach and I worked on better time management as well as expectations for the assignment. We were able to communicate without any hiccups, and meeting up in person was ideal for clarifying issues.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; We were unsure of our progress in the project as our understanding of milestone 4 meant we needed to wait this week on others. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Checking in with our project teams/coders will help us understand how they are doing, as well as how our own progression is lining up.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 19:15, 20 November 2017 (PST)&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
This week, Blair and I worked on the coder milestones. We will work on integrating the actual other team&amp;#039;s parts as they start to finish. We also collaborated with Emma and Katie to make sure that we were on the same page. &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 12]]&amp;lt;br&amp;gt;&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; we were better about our time management as well as we communicated well. We met up together periodically to make sure that we were on the same page.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; We were a little confused on how to go about the project for our milestone considering that it was more of a waiting game this week rather than actual integration. So, we didn&amp;#039;t really know how to quantify our progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What we will do to fix it:&amp;#039;&amp;#039;&amp;#039; We maybe should have collaborated with the other teams a little bit more to be able and gauge how their projects are coming along.&lt;br /&gt;
&amp;lt;!----&lt;br /&gt;
===Week 14===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
===Week 15===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
---&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Bibliography==&lt;br /&gt;
===Paper 1===&lt;br /&gt;
Homma, T., Iwahashi, H., &amp;amp; Komatsu, Y. (2003). Yeast gene expression during growth at low temperature. &amp;#039;&amp;#039;Cryobiology&amp;#039;&amp;#039;, 46(3), 230-237. &lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/12818212&lt;br /&gt;
* PubMed Central:  not available&lt;br /&gt;
* Publisher Full Text (HTML):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=12818212&lt;br /&gt;
* Publisher Full Text (PDF): https://electra.lmu.edu:3555/S0011224003000282/1-s2.0-S0011224003000282-main.pdf?_tid=4c9b7cc0-c775-11e7-af71-00000aab0f26&amp;amp;acdnat=1510469407_3e02bee4266fd6a211ea3f6e27b460cf &lt;br /&gt;
* Copyright: 2003 by Science Direct; article is not Open Access&lt;br /&gt;
* Publisher:  Elsevier Science&lt;br /&gt;
* Availability: online at www.sciencedirect.com&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 2===&lt;br /&gt;
Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., ... &amp;amp; Fujita, K. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. &amp;#039;&amp;#039;Extremophiles&amp;#039;&amp;#039;, 10(2), 117-128.&lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/?term=Genome-wide+expression+analysis+of+yeast+response+during+exposure+to+4+degrees+C&lt;br /&gt;
* PubMed Central: not available&lt;br /&gt;
* Publisher Full Text (HTML): https://electra.lmu.edu:2529/article/10.1007/s00792-005-0480-1&lt;br /&gt;
* Publisher Full Text (PDF):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=16254683&lt;br /&gt;
* Copyright: 2005 by Springer-Verlag; article is not Open Access&lt;br /&gt;
* Publisher:  Springer-Verlag&lt;br /&gt;
* Availability: online&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 3===&lt;br /&gt;
Becerra, M., Lombardia, L. J., González‐Siso, M. I., Rodríguez‐Belmonte, E., Hauser, N. C., &amp;amp; Cerdán, M. E. (2003). Genome‐wide analysis of the yeast transcriptome upon heat and cold shock. Comparative and functional genomics, 4(4), 366-375.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/pdf/CFG-04-366.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML):&amp;#039;&amp;#039;&amp;#039; unavailable &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF):&amp;#039;&amp;#039;&amp;#039; http://downloads.hindawi.com/journals/ijg/2003/175356.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright:&amp;#039;&amp;#039;&amp;#039; Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher:&amp;#039;&amp;#039;&amp;#039; Hindawi Publishing Corporation&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability:&amp;#039;&amp;#039;&amp;#039; Open access&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; no&lt;br /&gt;
&lt;br /&gt;
===Paper 4===&lt;br /&gt;
Sahara, T., Goda, T., &amp;amp; Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract: &amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pubmed/?term=Comprehensive+expression+analysis+of+time-dependent+genetic+responses+in+yeast+cells+to+low+temperature&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central: &amp;#039;&amp;#039;&amp;#039; not available &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.long&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.full.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright: &amp;#039;&amp;#039;&amp;#039; &amp;quot;Other parties are welcome to copy, distribute, transmit and adapt the work — at no cost and without permission — for noncommercial use as long as they attribute the work to the original source using the citation above.&amp;quot; from http://www.jbc.org/site/misc/Copyright_Permission.xhtml&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher: &amp;#039;&amp;#039;&amp;#039; American Society for Biochemistry and Molecular Biology&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability: &amp;#039;&amp;#039;&amp;#039;All articles from this journal are free 1 year after publishing.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; No.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Acknowledgments==&lt;br /&gt;
#Our group collaborates on this page each week filling out our respective team journal assignments and project updates.&lt;br /&gt;
#*WE have a group chat to communicate problems, questions and ideas for our weekly assignments/milestones.&lt;br /&gt;
#We utilized the Calendar outline from the [[JASPAR the Friendly Ghost]] page, but changed the milestone/tasks due.&lt;br /&gt;
&lt;br /&gt;
==References==&lt;br /&gt;
*Meyer, R. (2015, July 09). The Secret Startup That Saved the Worst Website in America. Retrieved November 7, 2017, from https://www.theatlantic.com/technology/archive/2015/07/the-secret-startup- saved-healthcare-gov-the-worst-website-in-america/397784/&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;br /&gt;
&lt;br /&gt;
{{GRNsight Gene Page Project Links}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4957</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4957"/>
				<updated>2017-11-21T05:49:37Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Results */ added one bullet point before hierarchical cluster analysis&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
*Cold shock response in yeast has been researched quite a bit, but the authors claim that this work is the first to study the effects at 4&amp;amp;deg;C. The Authors believe that their work is the first to study cold-shock response in yeast at a temperature below 10&amp;amp;deg;C.  &lt;br /&gt;
*It is important to study organisms at low temperatures because many organisms are exposed to freezing or near-freezing temperatures in the natural world.  Some organisms (such as &amp;#039;&amp;#039;E. coli&amp;#039;&amp;#039;) are quite often exposed to these low temperatures during experimental preparation.  &lt;br /&gt;
*It is also important to study the differences between organisms that have never been exposed to cold temperatures and organisms that have been grown in these cold conditions.  This will allow us to determine if/how well yeast can adapt to the cold.&lt;br /&gt;
&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
*Growth in yeast cells slowed once they were moved from 25&amp;amp;deg;C to 4&amp;amp;deg;C.    &lt;br /&gt;
*Hierarchical cluster analysis can be used to determine which sets of data are most similar to each other.  The process begins by grouping the two most similar data sets together into a &amp;quot;cluster.&amp;quot; Data sets are then added one-by-one to the initial cluster in order of similarity, until all data is categorized into one cluster. &amp;#039;&amp;#039;This technique revealed which time-points have the greatest similarity in gene expression.&amp;#039;&amp;#039;&lt;br /&gt;
**Hours 12 and 24 have the most similar gene-expression profile, and therefore are clustered together first.  &lt;br /&gt;
**The next closest to this cluster is 48 hours, which can then be added to the 12/24h  cluster form another, bigger cluster.  &lt;br /&gt;
**Hour 6 is the next closest to this cluster. &lt;br /&gt;
**Then the expression profile of the yeast constantly exposed to 4&amp;amp;deg;C &lt;br /&gt;
**And finally, with the least similar gene expression, is the yeast constantly exposed to 35&amp;amp;deg;C.&lt;br /&gt;
&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4955</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4955"/>
				<updated>2017-11-21T05:47:15Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Results */ added first result bullet point and sub-points&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
*Cold shock response in yeast has been researched quite a bit, but the authors claim that this work is the first to study the effects at 4&amp;amp;deg;C. The Authors believe that their work is the first to study cold-shock response in yeast at a temperature below 10&amp;amp;deg;C.  &lt;br /&gt;
*It is important to study organisms at low temperatures because many organisms are exposed to freezing or near-freezing temperatures in the natural world.  Some organisms (such as &amp;#039;&amp;#039;E. coli&amp;#039;&amp;#039;) are quite often exposed to these low temperatures during experimental preparation.  &lt;br /&gt;
*It is also important to study the differences between organisms that have never been exposed to cold temperatures and organisms that have been grown in these cold conditions.  This will allow us to determine if/how well yeast can adapt to the cold.&lt;br /&gt;
&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
*Hierarchical cluster analysis can be used to determine which sets of data are most similar to each other.  The process begins by grouping the two most similar data sets together into a &amp;quot;cluster.&amp;quot; Data sets are then added one-by-one to the initial cluster in order of similarity, until all data is categorized into one cluster. &amp;#039;&amp;#039;This technique revealed which time-points have the greatest similarity in gene expression.&amp;#039;&amp;#039;&lt;br /&gt;
**Hours 12 and 24 have the most similar gene-expression profile, and therefore are clustered together first.  &lt;br /&gt;
**The next closest to this cluster is 48 hours, which can then be added to the 12/24h  cluster form another, bigger cluster.  &lt;br /&gt;
**Hour 6 is the next closest to this cluster. &lt;br /&gt;
**Then the expression profile of the yeast constantly exposed to 4&amp;amp;deg;C &lt;br /&gt;
**And finally, with the least similar gene expression, is the yeast constantly exposed to 35&amp;amp;deg;C.&lt;br /&gt;
&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4953</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4953"/>
				<updated>2017-11-21T05:34:40Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Experimental design of the microarray data */ removed instructions&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
*Cold shock response in yeast has been researched quite a bit, but the authors claim that this work is the first to study the effects at 4&amp;amp;deg;C. The Authors believe that their work is the first to study cold-shock response in yeast at a temperature below 10&amp;amp;deg;C.  &lt;br /&gt;
*It is important to study organisms at low temperatures because many organisms are exposed to freezing or near-freezing temperatures in the natural world.  Some organisms (such as &amp;#039;&amp;#039;E. coli&amp;#039;&amp;#039;) are quite often exposed to these low temperatures during experimental preparation.  &lt;br /&gt;
*It is also important to study the differences between organisms that have never been exposed to cold temperatures and organisms that have been grown in these cold conditions.  This will allow us to determine if/how well yeast can adapt to the cold.&lt;br /&gt;
&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4946</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4946"/>
				<updated>2017-11-21T05:08:47Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Importance and significance of this work */ added three bullet points&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
*Cold shock response in yeast has been researched quite a bit, but the authors claim that this work is the first to study the effects at 4&amp;amp;deg;C. The Authors believe that their work is the first to study cold-shock response in yeast at a temperature below 10&amp;amp;deg;C.  &lt;br /&gt;
*It is important to study organisms at low temperatures because many organisms are exposed to freezing or near-freezing temperatures in the natural world.  Some organisms (such as &amp;#039;&amp;#039;E. coli&amp;#039;&amp;#039;) are quite often exposed to these low temperatures during experimental preparation.  &lt;br /&gt;
*It is also important to study the differences between organisms that have never been exposed to cold temperatures and organisms that have been grown in these cold conditions.  This will allow us to determine if/how well yeast can adapt to the cold.&lt;br /&gt;
&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).  &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps. also, a Flow Chart&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4926</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4926"/>
				<updated>2017-11-21T04:34:39Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Experimental design of the microarray data */ added a few more steps&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*The cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**Hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
*Corrections were made to account for the variations in quality of the spots and &amp;quot;cut off was done at an average background 118 value of +2SD.&amp;quot;&lt;br /&gt;
*20% of the data was used to calculate the Lowess fit at each point of a log-intensity vs log-ratio plot.  The resulting curve was used to adjust the control value for each measurement.&lt;br /&gt;
*Multiple DNA microarray trial were run, and at least 5 independent cultures were used.&lt;br /&gt;
*Genes with hypridization ratios &amp;amp;gt; 2.0 and &amp;amp;lt; 0.5 in at least 3 out of 5 experiments were considered up-regulated or down-regulate (respectively).  &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps. also, a Flow Chart&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4920</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4920"/>
				<updated>2017-11-21T04:19:56Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Acknowledgements */ added acknowledgements&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*the cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps.&lt;br /&gt;
*Flow Chart&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
*I&amp;#039;d like to thank the team Data Analyst/my co-presenter [[user:emmatyrnauer|Emma Tyrnauer]] for working with me on the assignment this week.  We talked during class, over text, as well as during our meet-up on Monday afternoon.  &lt;br /&gt;
*I&amp;#039;d also like to thank my other team partners [[User:Bhamilton18|Blair Hamilton]] and [[user:zvanysse|Zach Van Ysseldyke]] for continuing to work diligently on their portion of the project.  We communicated during class and via text.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. &amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
[[User:Kwrigh35|Kwrigh35]] ([[User talk:Kwrigh35|talk]]) 20:19, 20 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4915</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4915"/>
				<updated>2017-11-21T04:08:44Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Experimental design of the microarray data */ added a few more steps&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*the cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  This solution was heated to 90&amp;amp;deg;C for three minutes.&lt;br /&gt;
**hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
*The solution was then placed onto the array and incubated overnight at 65&amp;amp;deg;C to allow hybridization to occur.  &lt;br /&gt;
*The microarray was scanned with a Scanarray 4000 scanner&lt;br /&gt;
*The spots were located on the chip by GenePix4000 software.  &lt;br /&gt;
&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps.&lt;br /&gt;
*Flow Chart&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=4913</id>
		<title>Lights, Camera, InterACTION!</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=4913"/>
				<updated>2017-11-21T04:02:36Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* References */ added paper from week 12 journal club presentation&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:PugNotDrugsLogo.png|400px|thumb|right|Pugs Not Drugs Logo]]&lt;br /&gt;
&lt;br /&gt;
==Team Members==&lt;br /&gt;
*[[user:bhamilton18|Blair Hamilton]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:emmatyrnauer|Emma Tyrnauer]] &amp;#039;&amp;#039;&amp;#039;-Data Analyst&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:kwrigh35|Katie Wright]] &amp;#039;&amp;#039;&amp;#039;-Project Manager/Quality Assurance&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:zvanysse|Zach Van Ysseldyke]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
==File Roundup==&lt;br /&gt;
*Week 11 presentation by Blair and Zach: [[media:The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf|The Secret Startup that Saved the Worst Website in America]]&lt;br /&gt;
&lt;br /&gt;
==Team Schedule== &amp;lt;!--Borrowed Format from JASPAR group--&amp;gt;&lt;br /&gt;
{| class =&amp;quot;wikitable&amp;quot;&lt;br /&gt;
|+November &amp;amp; December&lt;br /&gt;
|-&lt;br /&gt;
|Sunday&lt;br /&gt;
|Monday&lt;br /&gt;
|Tuesday&lt;br /&gt;
|Wednesday&lt;br /&gt;
|Thursday&lt;br /&gt;
|Friday&lt;br /&gt;
|Saturday&lt;br /&gt;
|-&lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
| &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt; C: PPT Presentation&amp;lt;br&amp;gt;PM/DA: Journal Articles&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;17&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;18&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;19&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;20&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;21&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;lt;s&amp;gt;C: Complete setup steps (Milestones 0-4)&amp;lt;br&amp;gt;PM/DA: Journal Club Presentations&amp;lt;/s&amp;gt;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;22&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;23&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;24&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;&amp;#039;&amp;#039;Thanksgiving Break&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;25&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;26&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;27&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;28&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: First Integration projected completion&amp;lt;br&amp;gt;PM: &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;29&amp;#039;&amp;#039;&amp;#039; &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;30&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM: &amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;1&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;2&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;3&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;4&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;5&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with groups, and further integrate as needed.&amp;lt;br&amp;gt;DA:&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;6&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;7&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;C: Check in with other groups about progress&amp;lt;br&amp;gt;PM: &amp;lt;br&amp;gt;DA: &lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;8&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;9&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;10&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;11&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;Final group Presentation &amp;#039;&amp;#039;2:00-4:00PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;12&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;13&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;14&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;15&amp;#039;&amp;#039;&amp;#039;&amp;lt;br&amp;gt;All deliverables due &amp;#039;&amp;#039;4:30PM&amp;#039;&amp;#039;&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;16&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Executive Summaries==&lt;br /&gt;
===Week 11===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
For this week, I worked with [[user:emmatyrnauer|Emma]] to find papers to use as reference in our final research paper.  Emma and I both found papers that are related to yeast cold shock reactions and most of them use microarray data.  I am excited to read these articles more in depth as we develop our research project and paper.  Further information can be found on my [[kwrigh35 Week 11|Week 11]] journal page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039; Using the three different sources was really helpful because I had never used &amp;quot;Web of Science&amp;quot; before.  It was good to learn about how different these results can be, especially with tweaking the search terms a little bit.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I had poor time management this week.  I waited until Monday to do the majority of the work and definitely should not have done so.  The rest of the project went well, however.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I will plan to meet up with Emma early on in the week so we can work together on our presentation.  This way I will be held accountable to get my work done earlier.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week Katie and I worked on the annotated bibliography (below). We chose papers that provided more information on yeast gene regulation in response to cold shock. Pub Med, Google Scholar, and Web of Science were utilized to perform basic searches using key words as well as advanced searches which helped to narrow the results. More detailed information can be found on my individual journal entry page: [[Emmatyrnauer Week 11]]. Meanwhile, Blair and Zach worked on coder journal club presentation. Construction of the group page was completed during class.&lt;br /&gt;
# Overall, this week went pretty smoothly; Katie and I communicated over text to determine which papers we were going to write the bibliographies for and were available to each other for any questions we had.&lt;br /&gt;
# There wasn&amp;#039;t really anything that didn&amp;#039;t work.&lt;br /&gt;
# Since we will be working this next week on our journal club presentation, it might be beneficial to meet in person.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:26, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
This week Zach and I created the Powerpoint presentation about &amp;#039;&amp;#039;The secret startup that saved the worst website in America&amp;#039;&amp;#039; article. We created individual outlines of the article on our respective pages, mine is as follows: [[Bhamilton18 Week 11]]. Our presentation is linked here: [[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]]&lt;br /&gt;
#Overall, our communication and diving up of tasks went well and smoothly. Meanwhile, Katie and Emma worked on the annotate bibliography. While I primarily worked with Zach our communication amongst group members went well and we hope to continue to do so for the future.&lt;br /&gt;
#Something that didn&amp;#039;t work as well was our time management. Next time we hope to have more class time and start working earlier on our portion of the assignment. &lt;br /&gt;
#For the future, we will continue to communicate in person and through messaging and will work on time management by starting earlier.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 23:35, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
[[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]] &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 11]]&amp;lt;br&amp;gt;&lt;br /&gt;
This week, Blair and I created a Powerpoint about the &amp;quot;Secret Startup that saved the worst website in America.&amp;quot; We read through the article and picked out the relevant themes and main messages to put on the PowerPoint. We wrote these themes and summaries out on our individual Journals. Then, we collaborated and made our PowerPoint. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; Blair and I&amp;#039;s communication was very strong and we knew exactly what was going on with one another. We knew when we were going to meet up and what was expected for each of us.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Blair and I missed the mark on our time management. We did not expect this assignment to take this long. Next time, we will make sure to start on assignments earlier.&lt;br /&gt;
#Like I said above, we will plan out exact times earlier on in the week that we can meet up&lt;br /&gt;
[[User:Zvanysse|Zvanysse]] ([[User talk:Zvanysse|talk]]) 23:44, 13 November 2017 (PST) &amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
===Week 12===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
As mentioned on my electronic notebook, [[Bhamilton18 Week 12]], this week&amp;#039;s assignment Zachary Van Ysseldyk and I worked on the Coder Milestones 0-4. Zach and I will be working on the integration of the website bringing together the other groups work to function on the GRNsight website. Katie and Emma worked on their respective journal club presentation, but we communicated to check base on how everyone is doing in the progression of the project. Zach and I met in person to collaborate on formatting of the team page, individual journal pages and project progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked well:&amp;#039;&amp;#039;&amp;#039; Zach and I worked on better time management as well as expectations for the assignment. We were able to communicate without any hiccups, and meeting up in person was ideal for clarifying issues.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t worked well:&amp;#039;&amp;#039;&amp;#039; We were unsure of our progress in the project as our understanding of milestone 4 meant we needed to wait this week on others. &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will we do to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; Checking in with our project teams/coders will help us understand how they are doing, as well as how our own progression is lining up.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 19:15, 20 November 2017 (PST)&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
This week, Blair and I worked on the coder milestones. We will work on integrating the actual other team&amp;#039;s parts as they start to finish. We also collaborated with Emma and Katie to make sure that we were on the same page. &amp;lt;br&amp;gt;&lt;br /&gt;
[[Zvanysse Week 12]]&amp;lt;br&amp;gt;&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; we were better about our time management as well as we communicated well. We met up together periodically to make sure that we were on the same page.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; We were a little confused on how to go about the project for our milestone considering that it was more of a waiting game this week rather than actual integration. So, we didn&amp;#039;t really know how to quantify our progress.&lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What we will do to fix it:&amp;#039;&amp;#039;&amp;#039; We maybe should have collaborated with the other teams a little bit more to be able and gauge how their projects are coming along.&lt;br /&gt;
&amp;lt;!----&lt;br /&gt;
===Week 14===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
===Week 15===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
---&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Bibliography==&lt;br /&gt;
===Paper 1===&lt;br /&gt;
Homma, T., Iwahashi, H., &amp;amp; Komatsu, Y. (2003). Yeast gene expression during growth at low temperature. &amp;#039;&amp;#039;Cryobiology&amp;#039;&amp;#039;, 46(3), 230-237. &lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/12818212&lt;br /&gt;
* PubMed Central:  not available&lt;br /&gt;
* Publisher Full Text (HTML):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=12818212&lt;br /&gt;
* Publisher Full Text (PDF): https://electra.lmu.edu:3555/S0011224003000282/1-s2.0-S0011224003000282-main.pdf?_tid=4c9b7cc0-c775-11e7-af71-00000aab0f26&amp;amp;acdnat=1510469407_3e02bee4266fd6a211ea3f6e27b460cf &lt;br /&gt;
* Copyright: 2003 by Science Direct; article is not Open Access&lt;br /&gt;
* Publisher:  Elsevier Science&lt;br /&gt;
* Availability: online at www.sciencedirect.com&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 2===&lt;br /&gt;
Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., ... &amp;amp; Fujita, K. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. &amp;#039;&amp;#039;Extremophiles&amp;#039;&amp;#039;, 10(2), 117-128.&lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/?term=Genome-wide+expression+analysis+of+yeast+response+during+exposure+to+4+degrees+C&lt;br /&gt;
* PubMed Central: not available&lt;br /&gt;
* Publisher Full Text (HTML): https://electra.lmu.edu:2529/article/10.1007/s00792-005-0480-1&lt;br /&gt;
* Publisher Full Text (PDF):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=16254683&lt;br /&gt;
* Copyright: 2005 by Springer-Verlag; article is not Open Access&lt;br /&gt;
* Publisher:  Springer-Verlag&lt;br /&gt;
* Availability: online&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 3===&lt;br /&gt;
Becerra, M., Lombardia, L. J., González‐Siso, M. I., Rodríguez‐Belmonte, E., Hauser, N. C., &amp;amp; Cerdán, M. E. (2003). Genome‐wide analysis of the yeast transcriptome upon heat and cold shock. Comparative and functional genomics, 4(4), 366-375.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/pdf/CFG-04-366.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML):&amp;#039;&amp;#039;&amp;#039; unavailable &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF):&amp;#039;&amp;#039;&amp;#039; http://downloads.hindawi.com/journals/ijg/2003/175356.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright:&amp;#039;&amp;#039;&amp;#039; Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher:&amp;#039;&amp;#039;&amp;#039; Hindawi Publishing Corporation&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability:&amp;#039;&amp;#039;&amp;#039; Open access&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; no&lt;br /&gt;
&lt;br /&gt;
===Paper 4===&lt;br /&gt;
Sahara, T., Goda, T., &amp;amp; Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract: &amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pubmed/?term=Comprehensive+expression+analysis+of+time-dependent+genetic+responses+in+yeast+cells+to+low+temperature&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central: &amp;#039;&amp;#039;&amp;#039; not available &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.long&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.full.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright: &amp;#039;&amp;#039;&amp;#039; &amp;quot;Other parties are welcome to copy, distribute, transmit and adapt the work — at no cost and without permission — for noncommercial use as long as they attribute the work to the original source using the citation above.&amp;quot; from http://www.jbc.org/site/misc/Copyright_Permission.xhtml&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher: &amp;#039;&amp;#039;&amp;#039; American Society for Biochemistry and Molecular Biology&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability: &amp;#039;&amp;#039;&amp;#039;All articles from this journal are free 1 year after publishing.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; No.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
==Acknowledgments==&lt;br /&gt;
#Our group collaborates on this page each week filling out our respective team journal assignments and project updates.&lt;br /&gt;
#*WE have a group chat to communicate problems, questions and ideas for our weekly assignments/milestones.&lt;br /&gt;
#We utilized the Calendar outline from the [[JASPAR the Friendly Ghost]] page, but changed the milestone/tasks due.&lt;br /&gt;
&lt;br /&gt;
==References==&lt;br /&gt;
*Meyer, R. (2015, July 09). The Secret Startup That Saved the Worst Website in America. Retrieved November 7, 2017, from https://www.theatlantic.com/technology/archive/2015/07/the-secret-startup- saved-healthcare-gov-the-worst-website-in-america/397784/&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;br /&gt;
&lt;br /&gt;
{{GRNsight Gene Page Project Links}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4912</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4912"/>
				<updated>2017-11-21T04:01:47Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Referneces */ added link to paper&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*the cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  &lt;br /&gt;
**hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps.&lt;br /&gt;
*Flow Chart&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4911</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4911"/>
				<updated>2017-11-21T03:58:08Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Referneces */ added references and template and new categories&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*the cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  &lt;br /&gt;
**hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps.&lt;br /&gt;
*Flow Chart&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
==Referneces==&lt;br /&gt;
*LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12&lt;br /&gt;
*Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M.,  Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128.&lt;br /&gt;
&lt;br /&gt;
{{template:kwrigh35}}&lt;br /&gt;
[[Category: Group Projects]]&lt;br /&gt;
[[Category: Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Template:Pugs_Not_Drugs&amp;diff=4909</id>
		<title>Template:Pugs Not Drugs</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Template:Pugs_Not_Drugs&amp;diff=4909"/>
				<updated>2017-11-21T03:48:22Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Navigational Links */ Changed the width of the table&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Navigational Links==&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;Background: honeydew&amp;quot;&lt;br /&gt;
|- &lt;br /&gt;
!style=&amp;quot;background: mediumseagreen;color:white&amp;quot;| Category &lt;br /&gt;
!style=&amp;quot;background: mediumseagreen;color:white&amp;quot;| Links&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Interwiki&amp;#039;&amp;#039;&amp;#039; ||style=&amp;quot;width:575px&amp;quot;| [[Main Page]] &amp;amp;emsp; [[Main_Page#Course_Schedule|Course schedule]] &amp;amp;emsp; [[Lights, Camera, InterACTION!]]&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Team Members&amp;#039;&amp;#039;&amp;#039; ||  [[User:Bhamilton18|Blair Hamilton]] &amp;amp;emsp; [[user:emmatyrnauer|Emma Tyrnauer]] &amp;amp;emsp; [[User:Kwrigh35|Katie Wright]] &amp;amp;emsp; [[user:zvanysse|Zach Van Ysseldyk]]&lt;br /&gt;
|-&lt;br /&gt;
| &amp;#039;&amp;#039;&amp;#039;GRNsight Gene Page&amp;#039;&amp;#039;&amp;#039; &amp;lt;br&amp;gt;  &amp;#039;&amp;#039;&amp;#039;Project Instructions&amp;#039;&amp;#039;&amp;#039; ||style=&amp;quot;width:500px&amp;quot;| [[GRNsight Gene Page Project | Main Instructions]] &amp;amp;emsp; [[GRNsight Gene Page Project Deliverables | Project Deliverables]] &amp;amp;emsp; [[Week 11]] &amp;amp;emsp; [[Week 12]] &amp;amp;emsp; [[Week 14]] &amp;amp;emsp; [[Week 15]]&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Guilds&amp;#039;&amp;#039;&amp;#039; || [[Project Manager]] &amp;amp;emsp; [[Quality Assurance]] &amp;amp;emsp; [[Data Analysis]] &amp;amp;emsp; [[Coder]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
[[category:Group Projects]]&lt;br /&gt;
[[category:Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4837</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4837"/>
				<updated>2017-11-21T00:44:30Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* New Terms */ added definition and citation for Trehalose&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses.  &lt;br /&gt;
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. &amp;#039;&amp;#039;eLS&amp;#039;&amp;#039;. John Wiley &amp;amp; Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]&lt;br /&gt;
#DEPC-treated water&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*the cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  &lt;br /&gt;
**hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps.&lt;br /&gt;
*Flow Chart&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
==Referneces==&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4830</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4830"/>
				<updated>2017-11-21T00:06:31Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Experimental design of the microarray data */ Added first two bullet points&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
*the cDNA microarrays contained targets for 5,952 genes&lt;br /&gt;
**cDNA microarray chip comes from &amp;quot;DNA Chip Research, Kanagawa, Japan&amp;quot;&lt;br /&gt;
*20&amp;amp;mu;L of cDNA was mixed with 40&amp;amp;mu;L of DEPC-treated water and 60&amp;amp;mu;L of 2&amp;amp;times; hybridization solution.  &lt;br /&gt;
**hybridization solution comes from &amp;quot;CyScribe first strand cDNA labeling kit&amp;quot;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps.&lt;br /&gt;
*Flow Chart&lt;br /&gt;
&lt;br /&gt;
====Results====&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
==Referneces==&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4820</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4820"/>
				<updated>2017-11-20T23:50:31Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* New Terms */ added one term&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#Trehalose&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps.&lt;br /&gt;
*Flow Chart&lt;br /&gt;
====Results====&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
==Referneces==&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4818</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4818"/>
				<updated>2017-11-20T23:33:00Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* +==Results of this study in comparison to the results of previous studies=== */ corrected heading syntax error&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps.&lt;br /&gt;
*Flow Chart&lt;br /&gt;
====Results====&lt;br /&gt;
====Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
==Referneces==&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4816</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4816"/>
				<updated>2017-11-20T23:31:46Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Results of this study in comparison to the results of previous studies= */ corrected heading level&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps.&lt;br /&gt;
*Flow Chart&lt;br /&gt;
====Results====&lt;br /&gt;
=+==Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
==Referneces==&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4815</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4815"/>
				<updated>2017-11-20T23:31:10Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Electronic Notebook */ added outline for section&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
===New Terms===&lt;br /&gt;
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term. &lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
#&lt;br /&gt;
===Article Outline===&lt;br /&gt;
====Importance and significance of this work====&lt;br /&gt;
====Experimental design of the microarray data====&lt;br /&gt;
including treatments, number of replicates (biological and/or technical), dye swaps.&lt;br /&gt;
*Flow Chart&lt;br /&gt;
====Results====&lt;br /&gt;
===Results of this study in comparison to the results of previous studies====&lt;br /&gt;
&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
==Referneces==&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4814</id>
		<title>Kwrigh35 Week 12</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Kwrigh35_Week_12&amp;diff=4814"/>
				<updated>2017-11-20T23:26:39Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: Created Page, added sections&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Electronic Notebook==&lt;br /&gt;
==Acknowledgements==&lt;br /&gt;
==Referneces==&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Template:Pugs_Not_Drugs&amp;diff=4678</id>
		<title>Template:Pugs Not Drugs</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Template:Pugs_Not_Drugs&amp;diff=4678"/>
				<updated>2017-11-16T23:40:08Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* Navigational Links */ added links to guilds&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;==Navigational Links==&lt;br /&gt;
&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;Background: honeydew&amp;quot;&lt;br /&gt;
|- &lt;br /&gt;
!style=&amp;quot;background: mediumseagreen;color:white&amp;quot;| Category &lt;br /&gt;
!style=&amp;quot;background: mediumseagreen;color:white&amp;quot;| Links&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Interwiki&amp;#039;&amp;#039;&amp;#039; ||style=&amp;quot;width:500px&amp;quot;| [[Main Page]] &amp;amp;emsp; [[Main_Page#Course_Schedule|Course schedule]] &amp;amp;emsp; [[Lights, Camera, InterACTION!]]&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Team Members&amp;#039;&amp;#039;&amp;#039; ||  [[User:Bhamilton18|Blair Hamilton]] &amp;amp;emsp; [[user:emmatyrnauer|Emma Tyrnauer]] &amp;amp;emsp; [[User:Kwrigh35|Katie Wright]] &amp;amp;emsp; [[user:zvanysse|Zach Van Ysseldyk]]&lt;br /&gt;
|-&lt;br /&gt;
| &amp;#039;&amp;#039;&amp;#039;GRNsight Gene Page&amp;#039;&amp;#039;&amp;#039; &amp;lt;br&amp;gt;  &amp;#039;&amp;#039;&amp;#039;Project Instructions&amp;#039;&amp;#039;&amp;#039; ||style=&amp;quot;width:500px&amp;quot;| [[GRNsight Gene Page Project | Main Instructions]] &amp;amp;emsp; [[GRNsight Gene Page Project Deliverables | Project Deliverables]] &amp;amp;emsp; [[Week 11]] &amp;amp;emsp; [[Week 12]] &amp;amp;emsp; [[Week 14]] &amp;amp;emsp; [[Week 15]]&lt;br /&gt;
|-&lt;br /&gt;
|&amp;#039;&amp;#039;&amp;#039;Guilds&amp;#039;&amp;#039;&amp;#039; || [[Project Manager]] &amp;amp;emsp; [[Quality Assurance]] &amp;amp;emsp; [[Data Analysis]] &amp;amp;emsp; [[Coder]]&lt;br /&gt;
|-&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
[[category:Group Projects]]&lt;br /&gt;
[[category:Lights, Camera, InterACTION!]]&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=4670</id>
		<title>Lights, Camera, InterACTION!</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=4670"/>
				<updated>2017-11-16T23:32:24Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: /* File Roundup */ added link to week 11 presentation&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:PugNotDrugsLogo.png|400px|thumb|right|Pugs Not Drugs Logo]]&lt;br /&gt;
&lt;br /&gt;
==Team Members==&lt;br /&gt;
*[[user:bhamilton18|Blair Hamilton]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:emmatyrnauer|Emma Tyrnauer]] &amp;#039;&amp;#039;&amp;#039;-Data Analyst&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:kwrigh35|Katie Wright]] &amp;#039;&amp;#039;&amp;#039;-Project Manager/Quality Assurance&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:zvanysse|Zach Van Ysseldyke]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
==File Roundup==&lt;br /&gt;
*Week 11 presentation by Blair and Zach: [[media:The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf|The Secret Startup that Saved the Worst Website in America]]&lt;br /&gt;
&lt;br /&gt;
==Team Schedule==&lt;br /&gt;
&lt;br /&gt;
==Executive Summaries==&lt;br /&gt;
===Week 11===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
For this week, I worked with [[user:emmatyrnauer|Emma]] to find papers to use as reference in our final research paper.  Emma and I both found papers that are related to yeast cold shock reactions and most of them use microarray data.  I am excited to read these articles more in depth as we develop our research project and paper.  Further information can be found on my [[kwrigh35 Week 11|Week 11]] journal page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039; Using the three different sources was really helpful because I had never used &amp;quot;Web of Science&amp;quot; before.  It was good to learn about how different these results can be, especially with tweaking the search terms a little bit.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I had poor time management this week.  I waited until Monday to do the majority of the work and definitely should not have done so.  The rest of the project went well, however.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I will plan to meet up with Emma early on in the week so we can work together on our presentation.  This way I will be held accountable to get my work done earlier.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week Katie and I worked on the annotated bibliography (below). We chose papers that provided more information on yeast gene regulation in response to cold shock. Pub Med, Google Scholar, and Web of Science were utilized to perform basic searches using key words as well as advanced searches which helped to narrow the results. More detailed information can be found on my individual journal entry page: [[Emmatyrnauer Week 11]]. Meanwhile, Blair and Zach worked on coder journal club presentation. Construction of the group page was completed during class.&lt;br /&gt;
# Overall, this week went pretty smoothly; Katie and I communicated over text to determine which papers we were going to write the bibliographies for and were available to each other for any questions we had.&lt;br /&gt;
# There wasn&amp;#039;t really anything that didn&amp;#039;t work.&lt;br /&gt;
# Since we will be working this next week on our journal club presentation, it might be beneficial to meet in person.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:26, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
#This week Zach and I created the Powerpoint presentation about &amp;#039;&amp;#039;The secret startup that saved the worst website in America&amp;#039;&amp;#039; article. We created individual outlines of the article on our respective pages, mine is as follows: [[Bhamilton18 Week 11]]. Our presentation is linked here: [[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]]&lt;br /&gt;
#Overall, our communication and diving up of tasks went well and smoothly. Meanwhile, Katie and Emma worked on the annotate bibliography. While I primarily worked with Zach our communication amongst group members went well and we hope to continue to do so for the future.&lt;br /&gt;
#Something that didn&amp;#039;t work as well was our time management. Next time we hope to have more class time and start working earlier on our portion of the assignment. &lt;br /&gt;
#For the future, we will continue to communicate in person and through messaging and will work on time management by starting earlier.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 23:35, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
[[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]] &amp;lt;br&amp;gt;&lt;br /&gt;
*This week, Blair and I created a Powerpoint about the &amp;quot;Secret Startup that saved the worst website in America.&amp;quot; We read through the article and picked out the relevant themes and main messages to put on the PowerPoint. We wrote these themes and summaries out on our individual Journals. Then, we collaborated and made our PowerPoint. &lt;br /&gt;
*&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; Blair and I&amp;#039;s communication was very strong and we knew exactly what was going on with one another. We knew when we were going to meet up and what was expected for each of us.&lt;br /&gt;
*&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Blair and I missed the mark on our time management. We did not expect this assignment to take this long. Next time, we will make sure to start on assignments earlier.&lt;br /&gt;
*Like I said above, we will plan out exact times earlier on in the week that we can meet up&lt;br /&gt;
[[User:Zvanysse|Zvanysse]] ([[User talk:Zvanysse|talk]]) 23:44, 13 November 2017 (PST) &amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
===Week 12===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&amp;lt;!----&lt;br /&gt;
===Week 14===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
===Week 15===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
---&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Bibliography==&lt;br /&gt;
===Paper 1===&lt;br /&gt;
Homma, T., Iwahashi, H., &amp;amp; Komatsu, Y. (2003). Yeast gene expression during growth at low temperature. &amp;#039;&amp;#039;Cryobiology&amp;#039;&amp;#039;, 46(3), 230-237. &lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/12818212&lt;br /&gt;
* PubMed Central:  not available&lt;br /&gt;
* Publisher Full Text (HTML):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=12818212&lt;br /&gt;
* Publisher Full Text (PDF): https://electra.lmu.edu:3555/S0011224003000282/1-s2.0-S0011224003000282-main.pdf?_tid=4c9b7cc0-c775-11e7-af71-00000aab0f26&amp;amp;acdnat=1510469407_3e02bee4266fd6a211ea3f6e27b460cf &lt;br /&gt;
* Copyright: 2003 by Science Direct; article is not Open Access&lt;br /&gt;
* Publisher:  Elsevier Science&lt;br /&gt;
* Availability: online at www.sciencedirect.com&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 2===&lt;br /&gt;
Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., ... &amp;amp; Fujita, K. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. &amp;#039;&amp;#039;Extremophiles&amp;#039;&amp;#039;, 10(2), 117-128.&lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/?term=Genome-wide+expression+analysis+of+yeast+response+during+exposure+to+4+degrees+C&lt;br /&gt;
* PubMed Central: not available&lt;br /&gt;
* Publisher Full Text (HTML): https://electra.lmu.edu:2529/article/10.1007/s00792-005-0480-1&lt;br /&gt;
* Publisher Full Text (PDF):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=16254683&lt;br /&gt;
* Copyright: 2005 by Springer-Verlag; article is not Open Access&lt;br /&gt;
* Publisher:  Springer-Verlag&lt;br /&gt;
* Availability: online&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 3===&lt;br /&gt;
Becerra, M., Lombardia, L. J., González‐Siso, M. I., Rodríguez‐Belmonte, E., Hauser, N. C., &amp;amp; Cerdán, M. E. (2003). Genome‐wide analysis of the yeast transcriptome upon heat and cold shock. Comparative and functional genomics, 4(4), 366-375.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/pdf/CFG-04-366.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML):&amp;#039;&amp;#039;&amp;#039; unavailable &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF):&amp;#039;&amp;#039;&amp;#039; http://downloads.hindawi.com/journals/ijg/2003/175356.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright:&amp;#039;&amp;#039;&amp;#039; Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher:&amp;#039;&amp;#039;&amp;#039; Hindawi Publishing Corporation&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability:&amp;#039;&amp;#039;&amp;#039; Open access&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; no&lt;br /&gt;
&lt;br /&gt;
===Paper 4===&lt;br /&gt;
Sahara, T., Goda, T., &amp;amp; Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract: &amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pubmed/?term=Comprehensive+expression+analysis+of+time-dependent+genetic+responses+in+yeast+cells+to+low+temperature&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central: &amp;#039;&amp;#039;&amp;#039; not available &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.long&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.full.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright: &amp;#039;&amp;#039;&amp;#039; &amp;quot;Other parties are welcome to copy, distribute, transmit and adapt the work — at no cost and without permission — for noncommercial use as long as they attribute the work to the original source using the citation above.&amp;quot; from http://www.jbc.org/site/misc/Copyright_Permission.xhtml&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher: &amp;#039;&amp;#039;&amp;#039; American Society for Biochemistry and Molecular Biology&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability: &amp;#039;&amp;#039;&amp;#039;All articles from this journal are free 1 year after publishing.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; No.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=4662</id>
		<title>Lights, Camera, InterACTION!</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=4662"/>
				<updated>2017-11-16T23:23:36Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: Removed Zach and Blair&amp;#039;s templates&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:PugNotDrugsLogo.png|400px|thumb|right|Pugs Not Drugs Logo]]&lt;br /&gt;
&lt;br /&gt;
==Team Members==&lt;br /&gt;
*[[user:bhamilton18|Blair Hamilton]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:emmatyrnauer|Emma Tyrnauer]] &amp;#039;&amp;#039;&amp;#039;-Data Analyst&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:kwrigh35|Katie Wright]] &amp;#039;&amp;#039;&amp;#039;-Project Manager/Quality Assurance&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:zvanysse|Zach Van Ysseldyke]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
==File Roundup==&lt;br /&gt;
&lt;br /&gt;
==Team Schedule==&lt;br /&gt;
&lt;br /&gt;
==Executive Summaries==&lt;br /&gt;
===Week 11===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
For this week, I worked with [[user:emmatyrnauer|Emma]] to find papers to use as reference in our final research paper.  Emma and I both found papers that are related to yeast cold shock reactions and most of them use microarray data.  I am excited to read these articles more in depth as we develop our research project and paper.  Further information can be found on my [[kwrigh35 Week 11|Week 11]] journal page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039; Using the three different sources was really helpful because I had never used &amp;quot;Web of Science&amp;quot; before.  It was good to learn about how different these results can be, especially with tweaking the search terms a little bit.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I had poor time management this week.  I waited until Monday to do the majority of the work and definitely should not have done so.  The rest of the project went well, however.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I will plan to meet up with Emma early on in the week so we can work together on our presentation.  This way I will be held accountable to get my work done earlier.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week Katie and I worked on the annotated bibliography (below). We chose papers that provided more information on yeast gene regulation in response to cold shock. Pub Med, Google Scholar, and Web of Science were utilized to perform basic searches using key words as well as advanced searches which helped to narrow the results. More detailed information can be found on my individual journal entry page: [[Emmatyrnauer Week 11]]. Meanwhile, Blair and Zach worked on coder journal club presentation. Construction of the group page was completed during class.&lt;br /&gt;
# Overall, this week went pretty smoothly; Katie and I communicated over text to determine which papers we were going to write the bibliographies for and were available to each other for any questions we had.&lt;br /&gt;
# There wasn&amp;#039;t really anything that didn&amp;#039;t work.&lt;br /&gt;
# Since we will be working this next week on our journal club presentation, it might be beneficial to meet in person.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:26, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
#This week Zach and I created the Powerpoint presentation about &amp;#039;&amp;#039;The secret startup that saved the worst website in America&amp;#039;&amp;#039; article. We created individual outlines of the article on our respective pages, mine is as follows: [[Bhamilton18 Week 11]]. Our presentation is linked here: [[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]]&lt;br /&gt;
#Overall, our communication and diving up of tasks went well and smoothly. Meanwhile, Katie and Emma worked on the annotate bibliography. While I primarily worked with Zach our communication amongst group members went well and we hope to continue to do so for the future.&lt;br /&gt;
#Something that didn&amp;#039;t work as well was our time management. Next time we hope to have more class time and start working earlier on our portion of the assignment. &lt;br /&gt;
#For the future, we will continue to communicate in person and through messaging and will work on time management by starting earlier.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 23:35, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
[[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]] &amp;lt;br&amp;gt;&lt;br /&gt;
*This week, Blair and I created a Powerpoint about the &amp;quot;Secret Startup that saved the worst website in America.&amp;quot; We read through the article and picked out the relevant themes and main messages to put on the PowerPoint. We wrote these themes and summaries out on our individual Journals. Then, we collaborated and made our PowerPoint. &lt;br /&gt;
*&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; Blair and I&amp;#039;s communication was very strong and we knew exactly what was going on with one another. We knew when we were going to meet up and what was expected for each of us.&lt;br /&gt;
*&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Blair and I missed the mark on our time management. We did not expect this assignment to take this long. Next time, we will make sure to start on assignments earlier.&lt;br /&gt;
*Like I said above, we will plan out exact times earlier on in the week that we can meet up&lt;br /&gt;
[[User:Zvanysse|Zvanysse]] ([[User talk:Zvanysse|talk]]) 23:44, 13 November 2017 (PST) &amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
===Week 12===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&amp;lt;!----&lt;br /&gt;
===Week 14===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
===Week 15===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
---&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Bibliography==&lt;br /&gt;
===Paper 1===&lt;br /&gt;
Homma, T., Iwahashi, H., &amp;amp; Komatsu, Y. (2003). Yeast gene expression during growth at low temperature. &amp;#039;&amp;#039;Cryobiology&amp;#039;&amp;#039;, 46(3), 230-237. &lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/12818212&lt;br /&gt;
* PubMed Central:  not available&lt;br /&gt;
* Publisher Full Text (HTML):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=12818212&lt;br /&gt;
* Publisher Full Text (PDF): https://electra.lmu.edu:3555/S0011224003000282/1-s2.0-S0011224003000282-main.pdf?_tid=4c9b7cc0-c775-11e7-af71-00000aab0f26&amp;amp;acdnat=1510469407_3e02bee4266fd6a211ea3f6e27b460cf &lt;br /&gt;
* Copyright: 2003 by Science Direct; article is not Open Access&lt;br /&gt;
* Publisher:  Elsevier Science&lt;br /&gt;
* Availability: online at www.sciencedirect.com&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 2===&lt;br /&gt;
Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., ... &amp;amp; Fujita, K. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. &amp;#039;&amp;#039;Extremophiles&amp;#039;&amp;#039;, 10(2), 117-128.&lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/?term=Genome-wide+expression+analysis+of+yeast+response+during+exposure+to+4+degrees+C&lt;br /&gt;
* PubMed Central: not available&lt;br /&gt;
* Publisher Full Text (HTML): https://electra.lmu.edu:2529/article/10.1007/s00792-005-0480-1&lt;br /&gt;
* Publisher Full Text (PDF):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=16254683&lt;br /&gt;
* Copyright: 2005 by Springer-Verlag; article is not Open Access&lt;br /&gt;
* Publisher:  Springer-Verlag&lt;br /&gt;
* Availability: online&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 3===&lt;br /&gt;
Becerra, M., Lombardia, L. J., González‐Siso, M. I., Rodríguez‐Belmonte, E., Hauser, N. C., &amp;amp; Cerdán, M. E. (2003). Genome‐wide analysis of the yeast transcriptome upon heat and cold shock. Comparative and functional genomics, 4(4), 366-375.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/pdf/CFG-04-366.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML):&amp;#039;&amp;#039;&amp;#039; unavailable &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF):&amp;#039;&amp;#039;&amp;#039; http://downloads.hindawi.com/journals/ijg/2003/175356.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright:&amp;#039;&amp;#039;&amp;#039; Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher:&amp;#039;&amp;#039;&amp;#039; Hindawi Publishing Corporation&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability:&amp;#039;&amp;#039;&amp;#039; Open access&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; no&lt;br /&gt;
&lt;br /&gt;
===Paper 4===&lt;br /&gt;
Sahara, T., Goda, T., &amp;amp; Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract: &amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pubmed/?term=Comprehensive+expression+analysis+of+time-dependent+genetic+responses+in+yeast+cells+to+low+temperature&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central: &amp;#039;&amp;#039;&amp;#039; not available &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.long&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.full.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright: &amp;#039;&amp;#039;&amp;#039; &amp;quot;Other parties are welcome to copy, distribute, transmit and adapt the work — at no cost and without permission — for noncommercial use as long as they attribute the work to the original source using the citation above.&amp;quot; from http://www.jbc.org/site/misc/Copyright_Permission.xhtml&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher: &amp;#039;&amp;#039;&amp;#039; American Society for Biochemistry and Molecular Biology&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability: &amp;#039;&amp;#039;&amp;#039;All articles from this journal are free 1 year after publishing.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; No.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	<entry>
		<id>https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=4656</id>
		<title>Lights, Camera, InterACTION!</title>
		<link rel="alternate" type="text/html" href="https://xmlpipedb.lmucs.io/biodb/fall2017/index.php?title=Lights,_Camera,_InterACTION!&amp;diff=4656"/>
				<updated>2017-11-16T23:22:11Z</updated>
		
		<summary type="html">&lt;p&gt;Kwrigh35: Deleted Dr. Dahlquist&amp;#039;s note on approved paper for presentation. Added two new sections: File roundup and Team Schedule.  Revealed space for week 12 executive summaries.&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:PugNotDrugsLogo.png|400px|thumb|right|Pugs Not Drugs Logo]]&lt;br /&gt;
&lt;br /&gt;
==Team Members==&lt;br /&gt;
*[[user:bhamilton18|Blair Hamilton]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:emmatyrnauer|Emma Tyrnauer]] &amp;#039;&amp;#039;&amp;#039;-Data Analyst&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:kwrigh35|Katie Wright]] &amp;#039;&amp;#039;&amp;#039;-Project Manager/Quality Assurance&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
*[[user:zvanysse|Zach Van Ysseldyke]] &amp;#039;&amp;#039;&amp;#039;-Coder&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
==File Roundup==&lt;br /&gt;
&lt;br /&gt;
==Team Schedule==&lt;br /&gt;
&lt;br /&gt;
==Executive Summaries==&lt;br /&gt;
===Week 11===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
For this week, I worked with [[user:emmatyrnauer|Emma]] to find papers to use as reference in our final research paper.  Emma and I both found papers that are related to yeast cold shock reactions and most of them use microarray data.  I am excited to read these articles more in depth as we develop our research project and paper.  Further information can be found on my [[kwrigh35 Week 11|Week 11]] journal page.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What worked?&amp;#039;&amp;#039;&amp;#039; Using the three different sources was really helpful because I had never used &amp;quot;Web of Science&amp;quot; before.  It was good to learn about how different these results can be, especially with tweaking the search terms a little bit.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I had poor time management this week.  I waited until Monday to do the majority of the work and definitely should not have done so.  The rest of the project went well, however.  &lt;br /&gt;
#&amp;#039;&amp;#039;&amp;#039;What will I do next to fix what didn&amp;#039;t work?&amp;#039;&amp;#039;&amp;#039; I will plan to meet up with Emma early on in the week so we can work together on our presentation.  This way I will be held accountable to get my work done earlier.&lt;br /&gt;
&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
This week Katie and I worked on the annotated bibliography (below). We chose papers that provided more information on yeast gene regulation in response to cold shock. Pub Med, Google Scholar, and Web of Science were utilized to perform basic searches using key words as well as advanced searches which helped to narrow the results. More detailed information can be found on my individual journal entry page: [[Emmatyrnauer Week 11]]. Meanwhile, Blair and Zach worked on coder journal club presentation. Construction of the group page was completed during class.&lt;br /&gt;
# Overall, this week went pretty smoothly; Katie and I communicated over text to determine which papers we were going to write the bibliographies for and were available to each other for any questions we had.&lt;br /&gt;
# There wasn&amp;#039;t really anything that didn&amp;#039;t work.&lt;br /&gt;
# Since we will be working this next week on our journal club presentation, it might be beneficial to meet in person.&lt;br /&gt;
[[User:Emmatyrnauer|Emmatyrnauer]] ([[User talk:Emmatyrnauer|talk]]) 20:26, 13 November 2017 (PST)&lt;br /&gt;
&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
#This week Zach and I created the Powerpoint presentation about &amp;#039;&amp;#039;The secret startup that saved the worst website in America&amp;#039;&amp;#039; article. We created individual outlines of the article on our respective pages, mine is as follows: [[Bhamilton18 Week 11]]. Our presentation is linked here: [[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]]&lt;br /&gt;
#Overall, our communication and diving up of tasks went well and smoothly. Meanwhile, Katie and Emma worked on the annotate bibliography. While I primarily worked with Zach our communication amongst group members went well and we hope to continue to do so for the future.&lt;br /&gt;
#Something that didn&amp;#039;t work as well was our time management. Next time we hope to have more class time and start working earlier on our portion of the assignment. &lt;br /&gt;
#For the future, we will continue to communicate in person and through messaging and will work on time management by starting earlier.&lt;br /&gt;
[[User:Bhamilton18|Bhamilton18]] ([[User talk:Bhamilton18|talk]]) 23:35, 13 November 2017 (PST)&lt;br /&gt;
{{Bhamilton18}}&lt;br /&gt;
&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
[[Media: The_Secret_Startup_that_Saved_the_Worst_Website_in_America.pdf | Zach and Blair&amp;#039;s Powerpoint]] &amp;lt;br&amp;gt;&lt;br /&gt;
*This week, Blair and I created a Powerpoint about the &amp;quot;Secret Startup that saved the worst website in America.&amp;quot; We read through the article and picked out the relevant themes and main messages to put on the PowerPoint. We wrote these themes and summaries out on our individual Journals. Then, we collaborated and made our PowerPoint. &lt;br /&gt;
*&amp;#039;&amp;#039;&amp;#039;Things that went well:&amp;#039;&amp;#039;&amp;#039; Blair and I&amp;#039;s communication was very strong and we knew exactly what was going on with one another. We knew when we were going to meet up and what was expected for each of us.&lt;br /&gt;
*&amp;#039;&amp;#039;&amp;#039;Things that didn&amp;#039;t go well:&amp;#039;&amp;#039;&amp;#039; Blair and I missed the mark on our time management. We did not expect this assignment to take this long. Next time, we will make sure to start on assignments earlier.&lt;br /&gt;
*Like I said above, we will plan out exact times earlier on in the week that we can meet up&lt;br /&gt;
[[User:Zvanysse|Zvanysse]] ([[User talk:Zvanysse|talk]]) 23:44, 13 November 2017 (PST) &amp;lt;br&amp;gt;&lt;br /&gt;
{{Template:Zvanysse}}&lt;br /&gt;
&lt;br /&gt;
===Week 12===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&amp;lt;!----&lt;br /&gt;
===Week 14===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
===Week 15===&lt;br /&gt;
====Katie - PM/QA====&lt;br /&gt;
====Emma - Data Analysis====&lt;br /&gt;
====Blair - Coder====&lt;br /&gt;
====Zach - Coder====&lt;br /&gt;
&lt;br /&gt;
---&amp;gt;&lt;br /&gt;
&lt;br /&gt;
==Bibliography==&lt;br /&gt;
===Paper 1===&lt;br /&gt;
Homma, T., Iwahashi, H., &amp;amp; Komatsu, Y. (2003). Yeast gene expression during growth at low temperature. &amp;#039;&amp;#039;Cryobiology&amp;#039;&amp;#039;, 46(3), 230-237. &lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/12818212&lt;br /&gt;
* PubMed Central:  not available&lt;br /&gt;
* Publisher Full Text (HTML):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=12818212&lt;br /&gt;
* Publisher Full Text (PDF): https://electra.lmu.edu:3555/S0011224003000282/1-s2.0-S0011224003000282-main.pdf?_tid=4c9b7cc0-c775-11e7-af71-00000aab0f26&amp;amp;acdnat=1510469407_3e02bee4266fd6a211ea3f6e27b460cf &lt;br /&gt;
* Copyright: 2003 by Science Direct; article is not Open Access&lt;br /&gt;
* Publisher:  Elsevier Science&lt;br /&gt;
* Availability: online at www.sciencedirect.com&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 2===&lt;br /&gt;
Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., ... &amp;amp; Fujita, K. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. &amp;#039;&amp;#039;Extremophiles&amp;#039;&amp;#039;, 10(2), 117-128.&lt;br /&gt;
* PubMed Abstract:  https://www.ncbi.nlm.nih.gov/pubmed/?term=Genome-wide+expression+analysis+of+yeast+response+during+exposure+to+4+degrees+C&lt;br /&gt;
* PubMed Central: not available&lt;br /&gt;
* Publisher Full Text (HTML): https://electra.lmu.edu:2529/article/10.1007/s00792-005-0480-1&lt;br /&gt;
* Publisher Full Text (PDF):  http://sq4ya5rf2q.search.serialssolutions.com/?V=1.0&amp;amp;sid=PubMed:LinkOut&amp;amp;pmid=16254683&lt;br /&gt;
* Copyright: 2005 by Springer-Verlag; article is not Open Access&lt;br /&gt;
* Publisher:  Springer-Verlag&lt;br /&gt;
* Availability: online&lt;br /&gt;
* Did LMU pay a fee for this article: yes&lt;br /&gt;
&lt;br /&gt;
===Paper 3===&lt;br /&gt;
Becerra, M., Lombardia, L. J., González‐Siso, M. I., Rodríguez‐Belmonte, E., Hauser, N. C., &amp;amp; Cerdán, M. E. (2003). Genome‐wide analysis of the yeast transcriptome upon heat and cold shock. Comparative and functional genomics, 4(4), 366-375.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central:&amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447359/pdf/CFG-04-366.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML):&amp;#039;&amp;#039;&amp;#039; unavailable &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF):&amp;#039;&amp;#039;&amp;#039; http://downloads.hindawi.com/journals/ijg/2003/175356.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright:&amp;#039;&amp;#039;&amp;#039; Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher:&amp;#039;&amp;#039;&amp;#039; Hindawi Publishing Corporation&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability:&amp;#039;&amp;#039;&amp;#039; Open access&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; no&lt;br /&gt;
&lt;br /&gt;
===Paper 4===&lt;br /&gt;
Sahara, T., Goda, T., &amp;amp; Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Abstract: &amp;#039;&amp;#039;&amp;#039; https://www.ncbi.nlm.nih.gov/pubmed/?term=Comprehensive+expression+analysis+of+time-dependent+genetic+responses+in+yeast+cells+to+low+temperature&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;PubMed Central: &amp;#039;&amp;#039;&amp;#039; not available &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (HTML): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.long&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher Full Text (PDF): &amp;#039;&amp;#039;&amp;#039; http://www.jbc.org/content/277/51/50015.full.pdf&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Copyright: &amp;#039;&amp;#039;&amp;#039; &amp;quot;Other parties are welcome to copy, distribute, transmit and adapt the work — at no cost and without permission — for noncommercial use as long as they attribute the work to the original source using the citation above.&amp;quot; from http://www.jbc.org/site/misc/Copyright_Permission.xhtml&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Publisher: &amp;#039;&amp;#039;&amp;#039; American Society for Biochemistry and Molecular Biology&lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Availability: &amp;#039;&amp;#039;&amp;#039;All articles from this journal are free 1 year after publishing.  &lt;br /&gt;
&lt;br /&gt;
&amp;#039;&amp;#039;&amp;#039;Did LMU pay a fee for this article:&amp;#039;&amp;#039;&amp;#039; No.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
{{template:Pugs Not Drugs}}&lt;/div&gt;</summary>
		<author><name>Kwrigh35</name></author>	</entry>

	</feed>