Our Favorite Gene

HSF1, Our Favorite Yeast Gene

(Saccharomyces cerevisiae)

Arash Lari & Mary Balducci

SGD

Standard Name: HSF1
Systematic name: YGL073W
Name Description: Named because it codes for one of two Heat Shock transcription Factors

Website	ID#
SGD	S000003041
UniProt	P10961
NCBI Gene Databse	852806
Ensembl	YGL073W

Website

ID#

DNA Sequence:

1 ATGAATAATG CTGCAAATAC AGGGACGACC AATGAGTCAA ACGTGAGCGA TGCTCCCCGT 61 ATTGAGCCTT TACCAAGCTT GAATGATGAT GACATTGAAA AAATCTTACA ACCGAACGAT 121 ATCTTTACGA CCGATCGTAC CGATGCAAGT ACTACATCTT CCACAGCCAT TGAAGATATT 181 ATTAACCCCT CATTGGATCC GCAGTCAGCA GCATCGCCGG TTCCTTCTTC CTCTTTTTTC 241 CATGACTCAA GGAAACCTTC CACCAGTACA CATTTAGTAA GGAGAGGTAC TCCATTGGGA 301 ATTTACCAAA CCAATCTATA CGGTCACAAT AGCAGAGAAA ATACTAATCC TAATAGTACA 361 TTATTATCTT CTAAGTTACT CGCGCATCCA CCAGTTCCTT ATGGGCAAAA TCCCGATTTA 421 CTACAACATG CTGTGTACAG GGCACAGCCG TCAAGTGGAA CCACTAACGC GCAACCGCGC 481 CAAACCACAA GAAGATATCA ATCCCATAAA TCACGGCCTG CATTTGTTAA TAAACTATGG 541 AGCATGTTAA ACGATGATTC TAATACGAAA CTTATACAGT GGGCGGAGGA TGGAAAATCT 601 TTTATTGTCA CGAATAGGGA GGAATTTGTG CACCAAATTT TACCAAAATA TTTTAAACAT 661 TCCAATTTCG CTTCCTTTGT AAGACAATTG AACATGTATG GATGGCATAA AGTTCAAGAT 721 GTCAAGTCAG GATCAATTCA AAGTAGTTCA GATGATAAGT GGCAATTTGA AAATGAAAAC 781 TTCATTAGAG GTAGAGAAGA TTTGCTGGAA AAAATAATCA GGCAGAAAGG TTCCTCCAAT 841 AACCATAATA GCCCTAGTGG TAACGGTAAT CCAGCGAATG GTAGCAACAT CCCTCTGGAC 901 AATGCCGCAG GAAGTAATAA TAGCAATAAT AACATCAGTA GTAGTAATTC ATTTTTTAAC 961 AATGGTCATT TATTGCAGGG TAAAACACTA AGATTAATGA ACGAAGCGAA TCTTGGAGAT 1021 AAGAATGATG TCACCGCGAT TTTGGGGGAA TTAGAGCAAA TAAAATATAA CCAGATTGCA 1081 ATTTCCAAAG ATTTACTAAG AATAAACAAA GATAATGAGT TATTATGGCA AGAGAATATG 1141 ATGGCCAGGG AAAGACATAG AACCCAACAG CAAGCCTTGG AAAAAATGTT CAGATTCTTG 1201 ACATCTATAG TCCCACACTT AGATCCCAAA ATGATTATGG ACGGGCTGGG AGATCCGAAA 1261 GTTAATAATG AAAAGCTAAA CAGTGCGAAT AACATTGGGT TAAATCGCGA CAACACAGGC 1321 ACTATAGATG AACTAAAATC CAACGATTCT TTCATAAACG ATGATCGTAA TTCTTTCACC 1381 AATGCTACAA CCAACGCCCG TAATAACATG AGTCCCAACA ATGATGACAA TAGTATTGAC 1441 ACCGCTAGCA CTAATACCAC CAACAGAAAG AAAAATATAG ATGAAAACAT CAAAAATAAC 1501 AACGACATAA TTAATGACAT TATATTTAAT ACCAACCTTG CCAACAATCT CAGCAATTAC 1561 AATTCCAACA ATAATGCTGG CTCGCCAATA AGGCCCTATA AACAAAGATA TCTTTTGAAA 1621 AATAGAGCCA ATTCCTCGAC ATCGAGTGAG AATCCAAGCC TAACGCCCTT TGATATCGAA 1681 TCTAATAATG ACCGCAAAAT TTCAGAAATT CCTTTTGATG ACGAAGAAGA AGAAGAAACG 1741 GATTTTAGGC CTTTTACCTC GCGAGATCCT AATAACCAAA CGAGTGAAAA CACTTTTGAT 1801 CCAAACAGAT TTACGATGCT CTCTGATGAT GATTTAAAAA AAGATTCTCA TACCAATGAC 1861 AATAAACACA ACGAAAGTGA TCTTTTTTGG GACAACGTAC ATAGAAATAT AGACGAACAA 1921 GATGCAAGAC TCCAGAACTT GGAAAATATG GTTCACATAC TTTCTCCTGG ATATCCTAAT 1981 AAGTCGTTCA ACAACAAAAC TTCCTCGACA AACACTAATT CCAATATGGA AAGTGCTGTC 2041 AACGTTAATA GCCCTGGTTT CAACTTACAG GATTATTTAA CTGGAGAGTC TAATTCCCCC 2101 AATTCTGTTC ATTCTGTTCC CTCCAATGGC AGCGGCTCCA CACCGTTGCC CATGCCAAAT 2161 GATAATGACA CCGAGCACGC AAGTACAAGT GTCAATCAAG GCGAAAATGG AAGCGGATTA 2221 ACGCCCTTCC TCACGGTAGA TGATCACACA CTAAACGACA ATAACACTAG TGAGGGAAGT 2281 ACAAGGGTGT CCCCCGATAT AAAGTTCAGC GCCACTGAAA ACACTAAAGT GAGTGATAAC 2341 CTGCCAAGCT TTAATGACCA CAGTTATTCC ACCCAGGCCG ACACGGCGCC CGAGAACGCT 2401 AAGAAAAGAT TTGTGGAGGA AATACCGGAA CCGGCTATAG TCGAAATACA GGACCCGACA 2461 GAGTACAACG ATCACCGCCT GCCCAAACGA GCTAAGAAAT AG

Protein Sequence

1 MNNAANTGTT NESNVSDAPR IEPLPSLNDD DIEKILQPND IFTTDRTDAS TTSSTAIEDI 61 INPSLDPQSA ASPVPSSSFF HDSRKPSTST HLVRRGTPLG IYQTNLYGHN SRENTNPNST 121 LLSSKLLAHP PVPYGQNPDL LQHAVYRAQP SSGTTNAQPR QTTRRYQSHK SRPAFVNKLW 181 SMLNDDSNTK LIQWAEDGKS FIVTNREEFV HQILPKYFKH SNFASFVRQL NMYGWHKVQD 241 VKSGSIQSSS DDKWQFENEN FIRGREDLLE KIIRQKGSSN NHNSPSGNGN PANGSNIPLD 301 NAAGSNNSNN NISSSNSFFN NGHLLQGKTL RLMNEANLGD KNDVTAILGE LEQIKYNQIA 361 ISKDLLRINK DNELLWQENM MARERHRTQQ QALEKMFRFL TSIVPHLDPK MIMDGLGDPK 421 VNNEKLNSAN NIGLNRDNTG TIDELKSNDS FINDDRNSFT NATTNARNNM SPNNDDNSID 481 TASTNTTNRK KNIDENIKNN NDIINDIIFN TNLANNLSNY NSNNNAGSPI RPYKQRYLLK 541 NRANSSTSSE NPSLTPFDIE SNNDRKISEI PFDDEEEEET DFRPFTSRDP NNQTSENTFD 601 PNRFTMLSDD DLKKDSHTND NKHNESDLFW DNVHRNIDEQ DARLQNLENM VHILSPGYPN 661 KSFNNKTSST NTNSNMESAV NVNSPGFNLQ DYLTGESNSP NSVHSVPSNG SGSTPLPMPN 721 DNDTEHASTS VNQGENGSGL TPFLTVDDHT LNDNNTSEGS TRVSPDIKFS ATENTKVSDN 781 LPSFNDHSYS TQADTAPENA KKRFVEEIPE PAIVEIQDPT EYNDHRLPKR AKK*

More Information

Function:

This gene encodes a transcription factor in response to stress, especially heat shock related stress. This gene is necessary for the yeast’s survival. In cells which are not under stress, the gene is not active, but if the cell becomes under stresses such as heat, pH, or starvation, it becomes active to regulate the transcription of other genes, which work to protect the organism.

Differences:

The Saccharomyces Genome Database by far had the most information on this gene compared to the other three databases. The SGD website says that HSF1 is inactive in normal conditions, but the UniProt website says that this gene is necessary for normal growth. The NCBI Gene website has the same information as the SGD site, with little or no differences. The Ensembl website also does not have very much information on the function of the gene, what it did have was the same as the SGD website. I could not find anything under “Gene Expression” on this website.

Presentation:

The SGD website presented the information very clearly. The tabs at the top as well as the side menu made it easy to navigate each section of the website to find the information I was looking for. The UniProt website was also pretty simple, it had a list on the side where I could choose what I wanted to see, which also made finding information easy. The NCBI website also had a table of contents where I could click to which section i wanted, making it easy to find their information on the gene’s function. The Ensembl website also had a menu, but it was slightly less simple. Some of the categories in the menu did not come up with any results when I clicked them.

Why did we choose this gene?

We chose this gene by reading through the blog posts on the SGD website. They mentioned that this one was used in cases of heat stress. I think it’s interesting that there are genes that are prepared to regulate other genes in response to high levels of heat or other stressors, so I chose this one. My partner agreed that this was interesting and we both wanted to learn more.

An image of bread to represent yeast, as there were no pictures of hsf1 that were ok to use