Simonwro120 Week 6 - Revision history

Simonwro120: Finished Assignment

2017-10-09T20:12:50Z

Finished Assignment

Simonwro120: Answered Q9 added last sections

2017-10-09T01:01:33Z

Answered Q9 added last sections

Simonwro120: answered Q7

2017-10-08T23:45:38Z

answered Q7

Simonwro120: Answered Q6

2017-10-08T23:43:05Z

Answered Q6

Simonwro120: Answered Q4

2017-10-08T23:29:00Z

Answered Q4

Simonwro120: answered Q3

2017-10-08T23:02:13Z

answered Q3

Simonwro120: Answered Q2

2017-10-08T22:59:41Z

Answered Q2

Simonwro120: answered Q1

2017-10-08T22:46:12Z

answered Q1

New page

==BioLogical Database Reading Questions==
===Question 1===
[[Image:Graph1Week6.png|600px]]
===Question 2===
'''Gene X'''hour 1: 1.0 ratio, black. Hour 3: 2.2 ratio, color.

@@ Line 21: / Line 21: @@
 *Yes, genes X and Y were transcribed similarly. They both have a spike in transcriptions and then quickly fall nearing 0 by the end of hour 9.
 ===Question 4===
-*Typically, an image will be a bit more red or green as time goes on. At the start however the ratio will be closer to 1:1 so the merged image will display yellow. The yellow color comes from the images merging their own color on the computer. Figure 4.13 clearly shows that some genes change their expression levels when they realize they are starving, so as oxygen is reduced the ratio will either change to green or red. However until those signals are sent, the merged image has a close ratio of 1:1.
+*A 1:1 ratio of the green and red cDNAs is indicated by a yellow pigment when the images merge. The yellow color comes from the images merging their own color on the computer. Figure 4.13 clearly shows that some genes change their expression levels when they realize they are starving, so as oxygen is reduced the ratio will either change to green or red. However, until those signals are sent, the merged image has a close ratio of 1:1.
 ===Question 5===
 *According to OD 3.7 in Figure 4.12, TEF4 is depicted as repressed. This is possibly because TEF4 needs glucose to be able to transcribe, so as glucose supplies are reduced, the TEF4 gene's ability to transcribe reduce as well.
 ===Question 6===
-The TCA cycle genes would be induced so that they may use the glucose before it is all depleted.
+*As glucose supplies are depleted, an increase in the production of acetyl-CoA is caused by alcohol dehydrogenase. This results in more molecules entering into the TCA cycle. To handle the increase in molecules, TCA cycle genes would be induced.
 ===Question 7===
-The gene can use the "guilt by association" mechanism to ensure genes for enzymes in a common pathway are induced or repressed simultaneously.
+*The genome is able to ensure induction or repression simultaneously if all the genes for enzymes in a common pathway are regulated by a similar transcription factor.
 ===Question 8===
 ===Question 9===
-Assuming that cells that over express the transcription factor of Yap1p were subjected to the same experiment of a timecourse of glucose depletion, the spots that represented Yap1p target genes would show up as green the later the time points of this experiment. This is because Yap1p confers environmental stress. I am assuming that glucose depletion is an environmental stress factor.
+*Assuming that the cells which over express the transcription factor of Yap1p were subjected to the same experiment, in which glucose is depleted over time, the spots that represented Yap1p target genes would show up green as time moves forward in this experiment. This is because Yap1p confers environmental stress. This answer makes the assumption that glucose depletion is an environmental stress factor.
 ===Question 10===
 ===Acknowledgements===
 ===References===

← Older revision		Revision as of 01:01, 9 October 2017
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	===Question 7===		===Question 7===
	The gene can use the "guilt by association" mechanism to ensure genes for enzymes in a common pathway are induced or repressed simultaneously.		The gene can use the "guilt by association" mechanism to ensure genes for enzymes in a common pathway are induced or repressed simultaneously.
		+	===Question 8===
		+	===Question 9===
		+	Assuming that cells that over express the transcription factor of Yap1p were subjected to the same experiment of a timecourse of glucose depletion, the spots that represented Yap1p target genes would show up as green the later the time points of this experiment. This is because Yap1p confers environmental stress. I am assuming that glucose depletion is an environmental stress factor.
		+	===Question 10===
		+	===Acknowledgements===
		+	===References===

← Older revision		Revision as of 23:45, 8 October 2017
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	===Question 6===		===Question 6===
	The TCA cycle genes would be induced so that they may use the glucose before it is all depleted.		The TCA cycle genes would be induced so that they may use the glucose before it is all depleted.
		+	===Question 7===
		+	The gene can use the "guilt by association" mechanism to ensure genes for enzymes in a common pathway are induced or repressed simultaneously.

← Older revision		Revision as of 23:43, 8 October 2017
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	===Question 4===		===Question 4===
	*Typically, an image will be a bit more red or green as time goes on. At the start however the ratio will be closer to 1:1 so the merged image will display yellow. The yellow color comes from the images merging their own color on the computer. Figure 4.13 clearly shows that some genes change their expression levels when they realize they are starving, so as oxygen is reduced the ratio will either change to green or red. However until those signals are sent, the merged image has a close ratio of 1:1.		*Typically, an image will be a bit more red or green as time goes on. At the start however the ratio will be closer to 1:1 so the merged image will display yellow. The yellow color comes from the images merging their own color on the computer. Figure 4.13 clearly shows that some genes change their expression levels when they realize they are starving, so as oxygen is reduced the ratio will either change to green or red. However until those signals are sent, the merged image has a close ratio of 1:1.
		+	===Question 5===
		+	*According to OD 3.7 in Figure 4.12, TEF4 is depicted as repressed. This is possibly because TEF4 needs glucose to be able to transcribe, so as glucose supplies are reduced, the TEF4 gene's ability to transcribe reduce as well.
		+	===Question 6===
		+	The TCA cycle genes would be induced so that they may use the glucose before it is all depleted.

← Older revision		Revision as of 23:29, 8 October 2017
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	===Question 3===		===Question 3===
	*Yes, genes X and Y were transcribed similarly. They both have a spike in transcriptions and then quickly fall nearing 0 by the end of hour 9.		*Yes, genes X and Y were transcribed similarly. They both have a spike in transcriptions and then quickly fall nearing 0 by the end of hour 9.
		+	===Question 4===
		+	*Typically, an image will be a bit more red or green as time goes on. At the start however the ratio will be closer to 1:1 so the merged image will display yellow. The yellow color comes from the images merging their own color on the computer. Figure 4.13 clearly shows that some genes change their expression levels when they realize they are starving, so as oxygen is reduced the ratio will either change to green or red. However until those signals are sent, the merged image has a close ratio of 1:1.

← Older revision		Revision as of 23:02, 8 October 2017
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	*Hour 5: 2.0 ratio, very dim red.		*Hour 5: 2.0 ratio, very dim red.
	*Hour 9: 2.0 ratio, very dim red.		*Hour 9: 2.0 ratio, very dim red.
		+	===Question 3===
		+	*Yes, genes X and Y were transcribed similarly. They both have a spike in transcriptions and then quickly fall nearing 0 by the end of hour 9.

@@ Line 3: / Line 3: @@
 [[Image:Graph1Week6.png|600px]]
 ===Question 2===
-'''Gene X'''hour 1: 1.0 ratio, black. Hour 3: 2.2 ratio, color.
+'''Gene X'''