Difference between revisions of "Hhinsch Week 6"
From LMU BioDB 2017
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*Gene x: Black | *Gene x: Black | ||
*Gene y: Black | *Gene y: Black | ||
*Gene z: Black | *Gene z: Black | ||
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*Gene x: Dim Red | *Gene x: Dim Red | ||
*Gene y: Medium Red | *Gene y: Medium Red | ||
*Gene z: Dim Red | *Gene z: Dim Red | ||
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| − | + | '''5 Hour:''' | |
*Gene x: Black | *Gene x: Black | ||
*Gene y: Dim Green, almost Black | *Gene y: Dim Green, almost Black | ||
*Gene z: Dim Red | *Gene z: Dim Red | ||
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*Gene x: Medium Green | *Gene x: Medium Green | ||
*Gene y: Bright Green | *Gene y: Bright Green | ||
Revision as of 04:29, 10 October 2017
Discovery Questions from Chapter 4
1 Hour:
- Gene x: Black
- Gene y: Black
- Gene z: Black
3 Hour:
- Gene x: Dim Red
- Gene y: Medium Red
- Gene z: Dim Red
5 Hour:
- Gene x: Black
- Gene y: Dim Green, almost Black
- Gene z: Dim Red
9 Hour:
- Gene x: Medium Green
- Gene y: Bright Green
- Gene z: Dim Red
- Yes Genes x and y were transcribed similarly, they both show a similar pattern which is a strike in transcription then nearing zero as time increases.
- Most spots would be yellow at the first time point because the ratio of the two cDNA’s is 1:1. The technical reason that spots show up as yellow is that there was no change in a gene’s transcription and experimental growth conditions. The yellow color technically comes from the computer placing a yellow color on the spots that are open reading frames. The biological reason for most of the spots showing up as yellow is that genes are expressed in both transcriptomes.
- Over the course of the experiment TEF4 was repressed. I hypothesize that the change of expression due to lack of glucose is,
