Difference between revisions of "Johnllopez Week 8"
(→Electronic Lab Notebook: Added the total # of data points (Step 19)) |
(Created a new section for Bonferonni and p value correction) |
||
| Line 11: | Line 11: | ||
Our total n would be 16 because we are analyzing 4 time points and we have 4 replicates. | Our total n would be 16 because we are analyzing 4 time points and we have 4 replicates. | ||
| + | |||
| + | After letting 16 = n, we applied the two following functions in order to receive our dSWI4_Fstat and dsWI4_p-values: =((n-5)/5)*(Y2-AD2) and =FDIST(AE2,5,n-5). | ||
| + | Next, I filtered through my p-value data to show only p-values less than 0.05. The result was 5475 records found. | ||
| + | |||
| + | ===Bonferonni and p value Correction=== | ||
| + | |||
==My Spreadsheet== | ==My Spreadsheet== | ||
[[File:JL BIOL367 Fall2017 Dahlquist-microarray-data-master 20171017.zip | Here]]is my document. | [[File:JL BIOL367 Fall2017 Dahlquist-microarray-data-master 20171017.zip | Here]]is my document. | ||
Revision as of 23:06, 23 October 2017
Contents
Electronic Lab Notebook
Experimental Design and Getting Ready
The strain comparison I used is dSWI_4, the dat individual dataset that you will analyze, the filename, the number of replicates for each strain and each time point in your data.
Classwork
The first steps I took to complete this assignment were performed in class as I followed along to Dr. Dahlquist's instructions. Note that each time the list below advances 1 number, I performed a save.
- After initially downloading the Excel document, I went through and deleted all of the columns that did not relate to me and my partner's strain (dsWI4). Then, I went through the data and replaced cells with "NA" with a blank string. There were 3641 replacements.
- I then created a new worksheet, naming it
Our total n would be 16 because we are analyzing 4 time points and we have 4 replicates.
After letting 16 = n, we applied the two following functions in order to receive our dSWI4_Fstat and dsWI4_p-values: =((n-5)/5)*(Y2-AD2) and =FDIST(AE2,5,n-5). Next, I filtered through my p-value data to show only p-values less than 0.05. The result was 5475 records found.
Bonferonni and p value Correction
My Spreadsheet
File:JL BIOL367 Fall2017 Dahlquist-microarray-data-master 20171017.zipis my document.
