Difference between revisions of "Aporras1 Electronic Notebook"

Revision as of 06:05, 11 September 2017

5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3' Template DNA strand (Top Strand)
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5' Complimentary DNA Strand (Bottom Strand)

3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5' RNA Strand (Top Strand)

5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3' RNA Strand (Bottom Strand)

• Completed letter by letter strand of the complimentary DNA using the template strand
• Made two RNA strands using the 5' to 3' DNA strand and the 3' to 5' DNA strand
  • changed T to U on the RNA strands

RNA Strand (Top Strand) Reading Frames

• Put 1 single space every 3 codons starting after a, after c, and after g to create the 3 different reading frames

(-1) 3'-gca uac gau uau ggu aca agg cgc aua uug ggu cgg cgg uca agg cga ccg ccg uaa aau-5'
A-Y-D-Y-G-T-R-R-I-L-G-R-R-S-R-R-P-P-STOP-N

(-2) 3'-g cau acg auu aug gua caa ggc gca uau ugg guc ggc ggu caa ggc gac cgc cgu aaa au-5'
g-H-T-I-M-V-Q-G-A-Y-W-V-G-G-Q-G-D-R-R-K-au

(-3) 3'-gc aua cga uua ugg uac aag gcg cau auu ggg ucg gcg guc aag gcg acc gcc gua aaa u-5'
gc-I-R-L-W-Y-K-A-H-I-G-S-A-V-K-A-T-A-V-K-u

RNA Strand (Bottom Strand)

• Put 1 single space every 3 codons starting after a, after c, and after g to create the 3 different reading frames

1. 5'-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3'
R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L

2. 5'-cg uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a-3'

3. 5'-c gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua-3'