Difference between revisions of "Bhamilton18 Electronic Notebook"
From LMU BioDB 2017
Bhamilton18 (talk | contribs) |
Bhamilton18 (talk | contribs) (Clarified "STOP" codon) |
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5'-c(skip) gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua(skip)-3’ (Strand 1) | 5'-c(skip) gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua(skip)-3’ (Strand 1) | ||
| − | 5'-V-S-Stop-Y-H-V-P-R-I-T-Q-P-P-V-P-L-A-A-F-3' | + | 5'-V-S-Stop|-Y-H-V-P-R-I-T-Q-P-P-V-P-L-A-A-F-3' <!--Bar for stopping the gene, but translated rest of it to show ability to understand--> |
'''Reading Frame +3:''' | '''Reading Frame +3:''' | ||
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5'-cg(skip) uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a(skip)-3’ (Strand 1) | 5'-cg(skip) uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a(skip)-3’ (Strand 1) | ||
| − | 5'-Y-A-N-T-M-F-R-V-Stop-P-S-R-Q-F-R-W-R-H-F-3' | + | 5'-Y-A-N-T-M-F-R-V-Stop|-P-S-R-Q-F-R-W-R-H-F-3' <!--Bar for stopping the gene, but translated rest of it to show ability to understand--> |
'''Reading Frame -1''' | '''Reading Frame -1''' | ||
3'-gca uac gau uau ggu aca agg cgc aua uug ggu cgg cgg uca agg cga ccg ccg uaa aau-5' (Strand 2) | 3'-gca uac gau uau ggu aca agg cgc aua uug ggu cgg cgg uca agg cga ccg ccg uaa aau-5' (Strand 2) | ||
| − | 3'-A-Y-D-Y-G-T-R-R-I-L-G-R-R-S-R-R-P-P-Stop-N-5' | + | 3'-A-Y-D-Y-G-T-R-R-I-L-G-R-R-S-R-R-P-P-Stop|-N-5' <!--Bar for stopping the gene, but translated rest of it to show ability to understand--> |
'''Reading Frame -2''' | '''Reading Frame -2''' | ||
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3'-I-R-L-W-Y-K-A-H-I-G-S-A-V-K-A-T-A-V-K-5' | 3'-I-R-L-W-Y-K-A-H-I-G-S-A-V-K-A-T-A-V-K-5' | ||
| + | |||
| + | '''Typically with the "STOP" codon the gene is no longer translated; however, for the purposes of this exercise I translated the rest of the RNA into the respective amino acid.''' | ||
<br> | <br> | ||
The following readings frames are: '''open reading frames:''' +1, -2, -3 <!--These strands do not contain a "stop" codon--> | The following readings frames are: '''open reading frames:''' +1, -2, -3 <!--These strands do not contain a "stop" codon--> | ||
Latest revision as of 03:46, 12 September 2017
Genetic Code
Original Strand:
5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’
Complementary Strand:
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'
RNA Strand:
5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3’ (Strand 1)
3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5' (Strand 2)
Reading Frames
Reading Frame +1
5'-cgu aug cua aua cca ugu ucc gcg uau aac cca gcc gcc agu ucc gcu ggc ggc auu uua-3’ (Strand 1)
5'- R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L-3'
Reading Frame + 2
5'-c(skip) gua ugc uaa uac cau guu ccg cgu aua acc cag ccg cca guu ccg cug gcg gca uuu ua(skip)-3’ (Strand 1)
5'-V-S-Stop|-Y-H-V-P-R-I-T-Q-P-P-V-P-L-A-A-F-3'
Reading Frame +3:
5'-cg(skip) uau gcu aau acc aug uuc cgc gua uaa ccc agc cgc cag uuc cgc ugg cgg cau uuu a(skip)-3’ (Strand 1)
5'-Y-A-N-T-M-F-R-V-Stop|-P-S-R-Q-F-R-W-R-H-F-3'
Reading Frame -1
3'-gca uac gau uau ggu aca agg cgc aua uug ggu cgg cgg uca agg cga ccg ccg uaa aau-5' (Strand 2)
3'-A-Y-D-Y-G-T-R-R-I-L-G-R-R-S-R-R-P-P-Stop|-N-5'
Reading Frame -2
3'-g(skip) cau acg auu aug gua caa ggc gca uau ugg guc ggc ggu caa ggc gac cgc cgu aaa au(skip)-5' (Strand 2)
3'-H-T-I-M-V-Q-G-A-Y-W-V-G-G-Q-G-D-R-R-K-5'
Reading Frame -3
3'-gc(skip) aua cga uua ugg uac aag gcg cau auu ggg ucg gcg guc aag gcg acc gcc gua aaa u(skip)-5' (Strand 2)
3'-I-R-L-W-Y-K-A-H-I-G-S-A-V-K-A-T-A-V-K-5'
Typically with the "STOP" codon the gene is no longer translated; however, for the purposes of this exercise I translated the rest of the RNA into the respective amino acid.
The following readings frames are: open reading frames: +1, -2, -3
