Difference between revisions of "Johnllopez Week 6"
From LMU BioDB 2017
(Added my answers for questions 8 and 9) |
(Added my response for question 10) |
||
| Line 9: | Line 9: | ||
# In a scenario where cells that overexpress the transcription factor Yap1p were subjected to the same experiment of a timecourse of glucose depletion where cells at t0 (plenty of glucose available) are labeled green and cells at later timepoints (glucose depleted) are labeled red, I would expect the spots that represented glucose-repressed genes to be in the later time points of this experiment to be colored red. | # In a scenario where cells that overexpress the transcription factor Yap1p were subjected to the same experiment of a timecourse of glucose depletion where cells at t0 (plenty of glucose available) are labeled green and cells at later timepoints (glucose depleted) are labeled red, I would expect the spots that represented glucose-repressed genes to be in the later time points of this experiment to be colored red. | ||
# In a scenario where cells that overexpress the transcription factor Yap1p were subjected to the same experiment of a timecourse of glucose depletion where cells at t0 (plenty of glucose available) are labeled green and cells at later timepoints (glucose depleted) are labeled red, I would expect the spots that represented Yap1p target genes to be in the later time points of this experiment to be colored red. | # In a scenario where cells that overexpress the transcription factor Yap1p were subjected to the same experiment of a timecourse of glucose depletion where cells at t0 (plenty of glucose available) are labeled green and cells at later timepoints (glucose depleted) are labeled red, I would expect the spots that represented Yap1p target genes to be in the later time points of this experiment to be colored red. | ||
| + | # By performing enough of the same experiments to confirm that there is in fact a pattern, you could verify that you had truly deleted TUP1 or overexpressed YAP1 in the experiments described in questions 8 and 9. | ||
| + | |||
==Acknowledgements and References== | ==Acknowledgements and References== | ||
===References=== | ===References=== | ||
https://www.khanacademy.org/test-prep/mcat/biomolecules/krebs-citric-acid-cycle-and-oxidative-phosphorylation/a/the-citric-acid-cycle-2 | https://www.khanacademy.org/test-prep/mcat/biomolecules/krebs-citric-acid-cycle-and-oxidative-phosphorylation/a/the-citric-acid-cycle-2 | ||
Revision as of 01:24, 10 October 2017
Reading Questions
- For Gene X: Black, Dim Red, Black, Dim Green. For Gene Y: Black, Medium Red, Bright Green, Dim Green. For Gene Z: Black, Dim Red, Dim Red, Dim Red
- I would say that none of these genes were transcribed similar. Although X and Y share color patterns, with the exception of the third ratio, none of the transcription patterns align that well.
- Because the yellow color comes from the indication that there is no change within the genes, it would indicate that the reason most spots are yellow at their first time point is due to the fact that no change has occurred.
- The image indicated that TEF4 was repressed due to the light green color. This might be the case due to an increasing lack of glucose. Since glucose is necessary for translation to occur in this scenario (as it is the only available food source), the cell would respond to this by having perofrming less translation in order to preserve glucose.
- With the supply of glucose decreasing, the cells begin to perform cellular respiration in order to preserve what supply remains so that the cell might have energy.
- The guilt by association method is a way for the genome use to that ensure genes for enzymes in a common pathway are induced or repressed simultaneously because despite not knowing what that gene is, the same induction/repression can occur.
- In a scenario where cells that overexpress the transcription factor Yap1p were subjected to the same experiment of a timecourse of glucose depletion where cells at t0 (plenty of glucose available) are labeled green and cells at later timepoints (glucose depleted) are labeled red, I would expect the spots that represented glucose-repressed genes to be in the later time points of this experiment to be colored red.
- In a scenario where cells that overexpress the transcription factor Yap1p were subjected to the same experiment of a timecourse of glucose depletion where cells at t0 (plenty of glucose available) are labeled green and cells at later timepoints (glucose depleted) are labeled red, I would expect the spots that represented Yap1p target genes to be in the later time points of this experiment to be colored red.
- By performing enough of the same experiments to confirm that there is in fact a pattern, you could verify that you had truly deleted TUP1 or overexpressed YAP1 in the experiments described in questions 8 and 9.
Acknowledgements and References
References
https://www.khanacademy.org/test-prep/mcat/biomolecules/krebs-citric-acid-cycle-and-oxidative-phosphorylation/a/the-citric-acid-cycle-2
