Difference between revisions of "Emmatyrnauer Week 12"

Revision as of 05:01, 20 November 2017

Ambient temperature affects gene regulation
Has led to the evolution of biochemical and physiological properties
Known cellular activities in response to:
- heat shock: heat shock proteins expressed more
- cold shock: decreased permeability of cell membrane, decreased protein synthesis and folding, and decreased enzymatic activity
Currently there aren't any studies that examine the effects of cold shock below 10 deg C on gene expression in yeast
Interested in this because preservation of organisms often occurs at less than 10 deg C (i.e. 4 deg C)
This study exposed yeast to temperature of 4 deg C and examines changes in gene expression using cDNA microarray
- Cluster analysis performed
  - Results include gene expression between 6 and 48 hours after 4C cold shock differs from culture kept at 35C or 4C. After cold shock, expression of certain genes was induced (e.g. energy preservation genes, phospholipid synthesis genes, and cell rescue genes)
Now the question is what are the cellular responses of yeast which have already adapted to/able to survive at 4C

@@ Line 7: / Line 7: @@
 **heat shock: heat shock proteins expressed more
 **cold shock: decreased permeability of cell membrane, decreased protein synthesis and folding, and decreased enzymatic activity
+*Currently there aren't any studies that examine the effects of cold shock below 10 deg C on gene expression in yeast
+*Interested in this because preservation of organisms often occurs at less than 10 deg C (i.e. 4 deg C)
+*This study exposed yeast to temperature of 4 deg C and examines changes in gene expression using cDNA microarray
+**Cluster analysis performed
+***Results include gene expression between 6 and 48 hours after 4C cold shock differs from culture kept at 35C or 4C. After cold shock, expression of certain genes was induced (e.g. energy preservation genes, phospholipid synthesis genes, and cell rescue genes)
+*Now the question is what are the cellular responses of yeast which have already adapted to/able to survive at 4C
 ===Materials and Methods===