Difference between revisions of "Kwrigh35 Week 12"

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(Experimental design of the microarray data: Added first two bullet points)
(New Terms: added definition and citation for Trehalose)
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Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term.  
 
Make a list of at least 10 terms for which you did not know the definitions when you first read the article.  Define each of the terms and provide a citation for each term.  
 
#Trehalose
 
#Trehalose
#
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#*A non-reducing dissacharide found in many organisms in each kingdom.  It is often used in stress response and as a transport suger, among other uses. 
 +
#*Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. ''eLS''. John Wiley & Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]
 +
#DEPC-treated water
 
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#
 
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Revision as of 00:44, 21 November 2017

Electronic Notebook

New Terms

Make a list of at least 10 terms for which you did not know the definitions when you first read the article. Define each of the terms and provide a citation for each term.

  1. Trehalose
    • A non-reducing dissacharide found in many organisms in each kingdom. It is often used in stress response and as a transport suger, among other uses.
    • Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. eLS. John Wiley & Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]
  2. DEPC-treated water

Article Outline

Importance and significance of this work

Experimental design of the microarray data

  • the cDNA microarrays contained targets for 5,952 genes
    • cDNA microarray chip comes from "DNA Chip Research, Kanagawa, Japan"
  • 20μL of cDNA was mixed with 40μL of DEPC-treated water and 60μL of 2× hybridization solution.
    • hybridization solution comes from "CyScribe first strand cDNA labeling kit"


including treatments, number of replicates (biological and/or technical), dye swaps.

  • Flow Chart

Results

Results of this study in comparison to the results of previous studies

Acknowledgements

Referneces