Difference between revisions of "Kwrigh35 Week 2"

Latest revision as of 19:43, 9 September 2017

The Genetic Code

Original DNA Sequence

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’

Complementary DNA Strand

3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'

flipped to read in 5' direction 

5'-uaaaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauacg-3'

Reading Frames

Frame +1

5’-cgu-aug-cua-aua-cca-ugu-ucc-gcg-uau-aac-cca-gcc-gcc-agu-ucc-gcu-ggc-ggc-auu-uua-3’ 

5'-R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L-3' open reading frame

Frame +2

5’-cg-uau-gcu-aau-acc-aug-uuc-cgc-gua-uaa-ccc-agc-cgc-cag-uuc-cgc-ugg-cgg-cau-uuu-a-3’

5'-Y-A-N-T-M-F-R-V-3' stop codon (uaa) after valine

Frame +3

5’-c-gua-ugc-uaa-uac-cau-guu-ccg-cgu-aua-acc-cag-ccg-cca-guu-ccg-cug-gcg-gca-uuu-ta-3’  

5'-V-C-3' stop codon (uaa) after cysteine

Frame -1

5'-uaa-aau-gcc-gcc-agc-gga-acu-ggc-ggc-ugg-guu-aua-cgc-gga-aca-ugg-uau-uag-cau-acg-3' 

first codon is stop

Frame -2

5'-ua-aaa-ugc-cgc-cag-cgg-aac-ugg-cgg-cug-ggu-uau-acg-cgg-aac-aug-gua-uua-gca-uac-g-3' 

5'-K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y-3' open reading frame

Frame -3

5'-u-aaa-aug-ccg-cca-gcg-gaa-cug-gcg-gcu-ggg-uua-uac-gcg-gaa-cau-ggu-auu-agc-aua-cg-3' 

5'-K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I-3' open reading frame

Acknowledgments

• I worked with my Homework partner Simon Wroblewski Friday afternoon in the computer lab. We compared our sequences to check for errors and talked about wiki formatting.
• While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source. Kwrigh35 (talk) 13:41, 8 September 2017 (PDT)

References

• LMU BioDB 2017. (2017). Week 2. Retrieved September 8, 2017, from 2

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