Difference between revisions of "Simonwro120 Week 6"
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===Question 7=== | ===Question 7=== | ||
The gene can use the "guilt by association" mechanism to ensure genes for enzymes in a common pathway are induced or repressed simultaneously. | The gene can use the "guilt by association" mechanism to ensure genes for enzymes in a common pathway are induced or repressed simultaneously. | ||
| + | ===Question 8=== | ||
| + | ===Question 9=== | ||
| + | Assuming that cells that over express the transcription factor of Yap1p were subjected to the same experiment of a timecourse of glucose depletion, the spots that represented Yap1p target genes would show up as green the later the time points of this experiment. This is because Yap1p confers environmental stress. I am assuming that glucose depletion is an environmental stress factor. | ||
| + | ===Question 10=== | ||
| + | ===Acknowledgements=== | ||
| + | ===References=== | ||
Revision as of 01:01, 9 October 2017
Contents
BioLogical Database Reading Questions
Question 1
Question 2
Gene X
- Hour 1: 1.0 ratio, black.
- Hour 3: 2.2 ratio, very dim red.
- Hour 5: 1.0 ratio, black.
- Hour 9: 0.15 ratio, medium green.
Gene Y
- Hour 1: 1.0 ratio, black.
- Hour 3: 4.5 ratio, dim red.
- Hour 5: 0.95 ratio, still black.
- Hour 9: 0.05 ratio, very bright green.
Gene Z
- Hour 1: 1.0 ratio, black.
- Hour 3: 1.5 ratio, very dim red.
- Hour 5: 2.0 ratio, very dim red.
- Hour 9: 2.0 ratio, very dim red.
Question 3
- Yes, genes X and Y were transcribed similarly. They both have a spike in transcriptions and then quickly fall nearing 0 by the end of hour 9.
Question 4
- Typically, an image will be a bit more red or green as time goes on. At the start however the ratio will be closer to 1:1 so the merged image will display yellow. The yellow color comes from the images merging their own color on the computer. Figure 4.13 clearly shows that some genes change their expression levels when they realize they are starving, so as oxygen is reduced the ratio will either change to green or red. However until those signals are sent, the merged image has a close ratio of 1:1.
Question 5
- According to OD 3.7 in Figure 4.12, TEF4 is depicted as repressed. This is possibly because TEF4 needs glucose to be able to transcribe, so as glucose supplies are reduced, the TEF4 gene's ability to transcribe reduce as well.
Question 6
The TCA cycle genes would be induced so that they may use the glucose before it is all depleted.
Question 7
The gene can use the "guilt by association" mechanism to ensure genes for enzymes in a common pathway are induced or repressed simultaneously.
Question 8
Question 9
Assuming that cells that over express the transcription factor of Yap1p were subjected to the same experiment of a timecourse of glucose depletion, the spots that represented Yap1p target genes would show up as green the later the time points of this experiment. This is because Yap1p confers environmental stress. I am assuming that glucose depletion is an environmental stress factor.
