Difference between revisions of "Kwrigh35 Week 12"
From LMU BioDB 2017
(→New Terms: added one term) |
(→Experimental design of the microarray data: Added first two bullet points) |
||
Line 16: | Line 16: | ||
====Importance and significance of this work==== | ====Importance and significance of this work==== | ||
====Experimental design of the microarray data==== | ====Experimental design of the microarray data==== | ||
+ | *the cDNA microarrays contained targets for 5,952 genes | ||
+ | **cDNA microarray chip comes from "DNA Chip Research, Kanagawa, Japan" | ||
+ | *20μL of cDNA was mixed with 40μL of DEPC-treated water and 60μL of 2× hybridization solution. | ||
+ | **hybridization solution comes from "CyScribe first strand cDNA labeling kit" | ||
+ | |||
+ | |||
including treatments, number of replicates (biological and/or technical), dye swaps. | including treatments, number of replicates (biological and/or technical), dye swaps. | ||
*Flow Chart | *Flow Chart | ||
+ | |||
====Results==== | ====Results==== | ||
====Results of this study in comparison to the results of previous studies==== | ====Results of this study in comparison to the results of previous studies==== |
Revision as of 00:06, 21 November 2017
Contents
Electronic Notebook
New Terms
Make a list of at least 10 terms for which you did not know the definitions when you first read the article. Define each of the terms and provide a citation for each term.
- Trehalose
Article Outline
Importance and significance of this work
Experimental design of the microarray data
- the cDNA microarrays contained targets for 5,952 genes
- cDNA microarray chip comes from "DNA Chip Research, Kanagawa, Japan"
- 20μL of cDNA was mixed with 40μL of DEPC-treated water and 60μL of 2× hybridization solution.
- hybridization solution comes from "CyScribe first strand cDNA labeling kit"
including treatments, number of replicates (biological and/or technical), dye swaps.
- Flow Chart