Difference between revisions of "Kwrigh35 Week 12"
From LMU BioDB 2017
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*the cDNA microarrays contained targets for 5,952 genes | *the cDNA microarrays contained targets for 5,952 genes | ||
**cDNA microarray chip comes from "DNA Chip Research, Kanagawa, Japan" | **cDNA microarray chip comes from "DNA Chip Research, Kanagawa, Japan" | ||
| − | *20μL of cDNA was mixed with 40μL of DEPC-treated water and 60μL of 2× hybridization solution. | + | *20μL of cDNA was mixed with 40μL of DEPC-treated water and 60μL of 2× hybridization solution. This solution was heated to 90°C for three minutes. |
**hybridization solution comes from "CyScribe first strand cDNA labeling kit" | **hybridization solution comes from "CyScribe first strand cDNA labeling kit" | ||
| − | + | *The solution was then placed onto the array and incubated overnight at 65°C to allow hybridization to occur. | |
| + | *The microarray was scanned with a Scanarray 4000 scanner | ||
| + | *The spots were located on the chip by GenePix4000 software. | ||
including treatments, number of replicates (biological and/or technical), dye swaps. | including treatments, number of replicates (biological and/or technical), dye swaps. | ||
Revision as of 04:08, 21 November 2017
Contents
Electronic Notebook
New Terms
Make a list of at least 10 terms for which you did not know the definitions when you first read the article. Define each of the terms and provide a citation for each term.
- Trehalose
- A non-reducing dissacharide found in many organisms in each kingdom. It is often used in stress response and as a transport suger, among other uses.
- Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. eLS. John Wiley & Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]
- DEPC-treated water
Article Outline
Importance and significance of this work
Experimental design of the microarray data
- the cDNA microarrays contained targets for 5,952 genes
- cDNA microarray chip comes from "DNA Chip Research, Kanagawa, Japan"
- 20μL of cDNA was mixed with 40μL of DEPC-treated water and 60μL of 2× hybridization solution. This solution was heated to 90°C for three minutes.
- hybridization solution comes from "CyScribe first strand cDNA labeling kit"
- The solution was then placed onto the array and incubated overnight at 65°C to allow hybridization to occur.
- The microarray was scanned with a Scanarray 4000 scanner
- The spots were located on the chip by GenePix4000 software.
including treatments, number of replicates (biological and/or technical), dye swaps.
- Flow Chart
Results
Results of this study in comparison to the results of previous studies
Acknowledgements
Referneces
- LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12
- Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128. Retrieved from https://electra.lmu.edu:2529/content/pdf/10.1007%2Fs00792-005-0480-1.pdf
Useful Links
| Category | Links |
|---|---|
| Interwiki | Main Page Katie Wright Course schedule Lights, Camera, InterACTION! |
| Katie Wright Weekly Assignment |
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| Weekly Assignment Instructions |
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