Difference between revisions of "Dbashour Week 12"
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| + | ==Article== | ||
| + | Sahara, T., Goda, T., & Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021. | ||
| + | |||
==List of 10 Unknown Words and Their Definitions== | ==List of 10 Unknown Words and Their Definitions== | ||
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==Outline of Article== | ==Outline of Article== | ||
| − | I. | + | I. Background Information on Purpose of Experiment |
| − | * | + | * Low temperatures are known to have several effects on biochemical and physiological properties in various cells |
| − | * | + | * Cold shock proteins are induced when cells are exposed to low temperatures in order to cope with the drastic change in environment |
| − | * | + | * In yeast, the NSR1, TIP1, and OLE1 genes have been identified as important cold-inducible genes through past research, but their low temperature-dependent gene expression and response are still unclear |
| − | * | + | * The purpose of this experiment is to analyze global gene expression in low temperature-exposed yeast cells using a yeast cDNA microarray to obtain fundamental information on low temperature response and low temperature-dependent gene expression in yeast cells |
| − | II. | + | II. Cold shock and microarray procedures of yeast samples |
* | * | ||
* | * | ||
Revision as of 08:43, 21 November 2017
Contents
Article
Sahara, T., Goda, T., & Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.
List of 10 Unknown Words and Their Definitions
Outline of Article
I. Background Information on Purpose of Experiment
- Low temperatures are known to have several effects on biochemical and physiological properties in various cells
- Cold shock proteins are induced when cells are exposed to low temperatures in order to cope with the drastic change in environment
- In yeast, the NSR1, TIP1, and OLE1 genes have been identified as important cold-inducible genes through past research, but their low temperature-dependent gene expression and response are still unclear
- The purpose of this experiment is to analyze global gene expression in low temperature-exposed yeast cells using a yeast cDNA microarray to obtain fundamental information on low temperature response and low temperature-dependent gene expression in yeast cells
II. Cold shock and microarray procedures of yeast samples
- FLOW CHART
III. Results and Discussion
IV. Conclusion
V. Further Implications
Deliverable
Journal Club Week 12 Presentation
