Difference between revisions of "Aporras1 Week 2"

Revision as of 04:08, 12 September 2017

created by: Antonio Porras

Electronic Notebook

The Genetic Code

Template (top strand) and Complimentary (bottom strand) strands of DNA

5'-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3'
3'-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5'

Negative RNA Strand

3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5'

Positive RNA Strand

5'-cguaugcuaauaccauguuccgcguauaacccagccgccaguuccgcuggcggcauuuua-3' RNA Strand (Bottom Strand)

Reading frames (+1), (-2), and (-3) are considered open reading frames because they do not have a STOP codon.

(+1) Frame

5'-R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L-3'

(+2) Frame

5'-c-V-C-STOP-Y-H-V-O-R-I-T-Q-P-P-V-P-L-A-A-F-ua-3'

(+3) Frame

5'-cg-Y-A-N-T-M-F-R-V-STOP-P-S-R-Q-F-R-W-R-H-F-a-3'

(-1) Frame

3'-A-Y-D-Y-G-T-R-R-I-L-G-R-R-S-R-R-P-P-STOP-N-5'

(-2) Frame

3'-g-H-T-I-M-V-Q-G-A-Y-W-V-G-G-Q-G-D-R-R-K-au-5'

(-3) Frame

3'-gc-I-R-L-W-Y-K-A-H-I-G-S-A-V-K-A-T-A-V-K-u-5'

Typically with the translation of RNA to amino acid, the translation would stop when the "STOP" sequence is reached.

Acknowledgements

1. Met outside of class with Blair Hamilton to discuss any questions we had prior to meeting and throughout the process of completing the Week 2 assignment.
2. Retrieved the Genetic Code used from the Week 2 page.

References

1. Genetic code. (2017, August 31). Retrieved September 09, 2017, from https://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table
2. LMU BioDB 2017. (2017). Week 2. Retrieved September 11, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_2 Genetic code. (2017, August 31).
3. Open reading frame. (2017, July 30). Retrieved September 09, 2017, from https://en.wikipedia.org/wiki/Open_reading_frame