Aporras1 Week 4

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User Page: Antonio Porras

Assignment Page: Week 4

Electronic Notebook

  1. I visited the SGD Database and we chose the CLN1 gene as the model organism.
  2. First, searched through information on the SGD database and found the following going from the top to the bottom of the page:
    • Overview: regulation of cell cycle promoting first growth phase to S phase.
    • Found on chromosome XIII; also provides the sequence.
    • Cyclin dependent protein kinase.
    • Found within the nucleus and the cytoplasm.
    • Encodes for a G1 cyclin which regulates the cell cycle.
    • Cyclins are essential because they activate CDKs at the correct times within the cell cycle.
    • Provides length of protein to be 546 a.a., PI to be 5.62, and molecular weight to be 62038.2
    • Another observation on the website, it provides additional literature regarding the study of CLN1.
  3. I then searched on the NCBI Gene Database and gathered what I thought to be relevant information going from the top to the bottom of the webpage:
    • A protein coding gene also known as YMR199W
    • The genomic sequence is NC_001145.3
    • Provides various interactions with other genes, however this list is extensive/exhaustive and not essential to understand the function of the gene.
    • Provides further links to PubMed articles on CLN1.
    • Ontology processes: cell cycle, cell division, G1/S transition of mitotic cell cycle, regulation of serine/threonine kinase activity.
    • Name of the protein: NP_013926
    • Name of mRNA: NM_001182706.1
  4. After exhausting NCBI, I then looked for CLN1 on Ensembl:
    • Mostly the same information found on prior websites but I'll include the information anyways.
    • Identifier: YMR199W
    • Involved in protein coding.
    • Found on chromosome 8 (XIII).
    • Found in the cytoplasm and the nucleus.
    • Involved in processes: cell cycle, cell division, and regulation of cyclin-dependent protein.
    • No phenotype associated with the gene.
    • Site provides a variant table of the gene, noted to be very extensive.
    • Involved in regulation of cell cycle: G1 to S phase.
    • As I was looking at the structure of the page, the links to each section were on the left hand side which made it slightly more work to navigate.
  5. I then searched on uniprot and will only list any information that hasn't yet been mentioned:
    • Organism: Saccharomyces cerevisiae (baker's yeast)
    • Belongs to the cyclin family.
    • Notices that it cites SGD and must draw from the same if not similar sources.

One Paragraph Summary on CLN1

CLN1 is a gene involved in protein coding found on the chromosome XIII. It encodes for a G1 cyclin which regulates the cell cycle meaning they activate CDKs at the correct times within the cell cycle. It can be found within the nucleus and the cytoplasm of the cell. To further characterize CLN1, the genomic sequence is NC_001145.3 and the name of the mRNA and protein Reference Sequences are NM_001182706.1 and NP_013926.1 respectively. Another primary way to identify the gene is through the locus name or tag: YMR199W. The processes CLN1 is involved in includes the cell cycle, cell division, and regulation of serine/threonine kinase activity. Lastly, to discuss the physical characteristics, the protein length was measured to be 546 a.a., the isoelectric point to be 5.62, and the molecular weight to be 62038.2 Da.

Acknowledgements

  1. Met outside of class with Eddie Azinge to discuss any questions we had prior to meeting and throughout the process of completing the Week 4 assignment.

While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source.

Aporras1 (talk) 21:03, 25 September 2017 (PDT)

References

  1. Ensembl.org. (2017). Gene: CLN1 (YMR199W) - Supporting evidence - Saccharomyces cerevisiae - Ensembl genome browser 90. [online] Available at: https://www.ensembl.org/Saccharomyces_cerevisiae/Gene/Evidence?db=core;g=YMR199W;r=XIII:662644-664284;t=YMR199W [Accessed 26 Sep. 2017].
  2. LMU BioDB 2017. (2017). Week 3. Retrieved September 16, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_3
  3. Ncbi.nlm.nih.gov. (2017). CLN1 cyclin CLN1 [Saccharomyces cerevisiae S288C] - Gene - NCBI. [online] Available at: https://www.ncbi.nlm.nih.gov/gene/855239 [Accessed 26 Sep. 2017].
  4. Yeastgenome.org. (2017). CLN1 | SGD. [online] Available at: https://www.yeastgenome.org/locus/S000004812 [Accessed 26 Sep. 2017].
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