Dbashour Week 12

Article

Sahara, T., Goda, T., & Ohgiya, S. (2002). Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. Journal of Biological Chemistry, 277(51), 50015-50021.

Outline of Article

I. Background Information on Purpose of Experiment

• Low temperatures are known to have several effects on biochemical and physiological properties in various cells
• Cold shock proteins are induced when cells are exposed to low temperatures in order to cope with the drastic change in environment
• In yeast, the NSR1, TIP1, and OLE1 genes have been identified as important cold-inducible genes through past research, but their low temperature-dependent gene expression and response are still unclear
• The purpose of this experiment is to analyze global gene expression in low temperature-exposed yeast cells using a yeast cDNA microarray to obtain fundamental information on low temperature response and low temperature-dependent gene expression in yeast cells

II. Cold shock and microarray procedures of yeast samples

• FLOW CHART

III. Results and Discussion

IV. Conclusion

V. Further Implications

Deliverable

Journal Club Week 12 Presentation