Simonwro120 Week 2

Journal Week 2

Genetic Code

Original DNA Sequence

5’-cgtatgctaataccatgttccgcgtataacccagccgccagttccgctggcggcatttta-3’

Complementary DNA Sequence

3’-gcatacgattatggtacaaggcgcatattgggtcggcggtcaaggcgaccgccgtaaaat-5’

Original Frame +1

5’-cgt-atg-cta-ata-cca-tgt-tcc-gcg-tat-aac-cca-gcc-gcc-agt-tcc-gct-ggc-ggc-att-tta-3’
5'-R-M-L-I-P-C-S-A-Y-N-P-A-A-S-S-A-G-G-I-L-3’
*This reading frame is open and 20 amino acids long

Original Frame +2

5’-cg-tat-gct-aat-acc-atg-ttc-cgc-gta-taa-ccc-agc-cgc-cag-ttc-cgc-tgg-cgg-cat-ttt-a-3’
5’-Y-A-N-T-M-F-R-V-3'   
*This reading frame is closed and 8 amino acids long

Original Frame +3

5’-c-gta-tgc-taa-tac-cat-gtt-ccg-cgt-ata-acc-cag-ccg-cca-gtt-ccg-ctg-gcg-gca-ttt-ta-3’
5’-V-C-3’
*This reading frame is closed and has 2 amino acids

Complementary RNA Strand

3'-gcauacgauuaugguacaaggcgcauauugggucggcggucaaggcgaccgccguaaaau-5' 
Flipped to be read from 5' to 3'
5'-uaaaaugccgccagcggaacuggcggcuggguuauacgcggaacaugguauuagcauacg-3'

Complementary Frame -1

5'-uaa-aau-gcc-gcc-agc-gga-acu-ggc-ggc-ugg-guu-aua-cgc-gga-aca-ugg-uau-uag-cau-acg-3' 

*First codon is a stop codon so the reading frame is closed

Complementary Frame -2

5'-ua-aaa-ugc-cgc-cag-cgg-aac-ugg-cgg-cug-ggu-uau-acg-cgg-aac-aug-gua-uua-gca-uac-g-3' 

5'-K-C-R-Q-R-N-W-R-L-G-Y-T-R-N-M-V-L-A-Y-3'
*This reading frame is open and 19 amino acids long

Complementary Frame -3

5'-u-aaa-aug-ccg-cca-gcg-gaa-cug-gcg-gcu-ggg-uua-uac-gcg-gaa-cau-ggu-auu-agc-aua-cg-3' 

5'-K-M-P-P-A-E-L-A-A-G-L-Y-A-E-H-G-I-S-I-3'
*This reading frame is open and 19 amino acids long

Acknowledgments

Worked with group partner Katie at Katie's Homepage All original journal entries came from me. Simonwro120 (talk) 13:28, 11 September 2017 (PDT) Dr. Dondi, and Dr. Dahlquist lectures

References

• LMU BioDB 2017. (2017). Week 2. Retrieved September 8, 2017, from [1]

Assignment link

• Assignment Week 2

Journal Entry