Hhinsch Week 6

Discovery Questions from Chapter 4

1 Hour:

• Gene x: Black
• Gene y: Black
• Gene z: Black

3 Hour:

• Gene x: Dim Red
• Gene y: Medium Red
• Gene z: Dim Red

5 Hour:

• Gene x: Black
• Gene y: Dim Green, almost Black
• Gene z: Dim Red

9 Hour:

• Gene x: Medium Green
• Gene y: Bright Green
• Gene z: Dim Red

1. Yes Genes x and y were transcribed similarly, they both show a similar pattern which is a strike in transcription then nearing zero as time increases.
2. Most spots would be yellow at the first time point because the ratio of the two cDNA’s is 1:1. The technical reason that spots show up as yellow is that there was no change in a gene’s transcription and experimental growth conditions. The yellow color technically comes from the computer placing a yellow color on the spots that are open reading frames. The biological reason for most of the spots showing up as yellow is that genes are expressed in both transcriptomes.
3. Over the course of the experiment TEF4 was repressed. I hypothesize that the change of expression due to lack of glucose is,