QLanners Week 8

number of NAs = 10,854

total n = 15

Data from the master sheet was missing sample #2 for both t=90 and had no t=120 data.

Data points at each time: t15 = 4 t30 = 4 t60 = 4 t90 = 3

number of timepoints = 4

Unadjusted p value

• Less than 0.05
  • 1630
  • Percentage= 26.34%
• Less than 0.01
  • 880
  • Percentage= 14.22%
• Less than 0.001
  • 356
  • Percentage= 5.75%
• Less than 0.0001
  • 142
  • Percentage= 2.29%

Bonferroni-corrected p value

• Less than 0.05
  • 53
  • Percentage= 0.856%

Benjamin and Hochberg-corrected p value

• Less than 0.05
  • 730
  • Percentage= 11.80%

NSR1 gene

• p values
  • unadjusted: 2.92024E-06
  • Bonferroni-corrected: 0.018073394
  • Benjamin and Hochberg-corrected: 0.000430319
• Average Log fold changes
  • At 15 min: 1.886696138
  • At 30 min: 2.611946831
  • At 60 min: 1.394879511
  • At 90 min: -2.673801047

The NSR1 gene most certainly changes expression due to the cold shock in this experiment. This is apparent from the large values for all of the Log fold changes at every time interval (as a 0 Log value would mean no change in expression and thus a large non-zero Log value means a large change in expression).

Media:DASH1 analysis QL.zip