Johnllopez Week 10
From LMU BioDB 2017
Revision as of 18:21, 6 November 2017 by Johnllopez616 (talk | contribs) (Elaborated further on Step 1)
Electronic Lab Notebook
Preparing My Microarray Data File for Loading into STEM
- I started this portion by downloading the following spreadsheets. I added a new worksheet named dSWI4_stem, selected the values from dSWI4_ANOVA, and copied them into the new worksheet.
- I modified this further by renaming the header "Master_Index" column to "SPOT", "ID" to "Gene Symbol", and deleting the "Standard_Name" column.
- I filtered the data on the B-H corrected p-value column to be greater than 0.05, and deleted all the data in the header row. Once I undid the filter, this ensured that all of the genes within the data set would have a B-H corrected p-value of <.05. The result was 2794 genes remaining.
- I then deleted all of the columns except for the Average Log Fold change columns at the timepoints. This would be used for [insert use] later on.
- I saved the spreadsheets as usal, then I saved it as a .txt file, which you can see [insert here].
