Kwrigh35 Week 12

Electronic Notebook

New Terms

Make a list of at least 10 terms for which you did not know the definitions when you first read the article. Define each of the terms and provide a citation for each term.

1. Trehalose
   • A non-reducing dissacharide found in many organisms in each kingdom. It is often used in stress response and as a transport suger, among other uses.
   • Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. eLS. John Wiley & Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]
2. DEPC-treated water

Article Outline

Experimental design of the microarray data

• the cDNA microarrays contained targets for 5,952 genes
  • cDNA microarray chip comes from "DNA Chip Research, Kanagawa, Japan"
• 20μL of cDNA was mixed with 40μL of DEPC-treated water and 60μL of 2× hybridization solution.
  • hybridization solution comes from "CyScribe first strand cDNA labeling kit"

including treatments, number of replicates (biological and/or technical), dye swaps.

• Flow Chart