Kwrigh35 Week 12

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Electronic Notebook

New Terms

Make a list of at least 10 terms for which you did not know the definitions when you first read the article. Define each of the terms and provide a citation for each term.

  1. Trehalose
    • A non-reducing dissacharide found in many organisms in each kingdom. It is often used in stress response and as a transport suger, among other uses.
    • Lunn, J. E. (Apr 2016). Sucrose Metabolism [Abstract]. eLS. John Wiley & Sons Ltd, Chichester. Retrieved from http://www.els.net [doi: 10.1002/9780470015902.a0021259.pub2]
  2. DEPC-treated water

Article Outline

Importance and significance of this work

Experimental design of the microarray data

  • the cDNA microarrays contained targets for 5,952 genes
    • cDNA microarray chip comes from "DNA Chip Research, Kanagawa, Japan"
  • 20μL of cDNA was mixed with 40μL of DEPC-treated water and 60μL of 2× hybridization solution.
    • hybridization solution comes from "CyScribe first strand cDNA labeling kit"


including treatments, number of replicates (biological and/or technical), dye swaps.

  • Flow Chart

Results

Results of this study in comparison to the results of previous studies

Acknowledgements

Referneces

  • LMU BioDB 2017. (2017). Week 12. Retrieved November 20, 2017, from https://xmlpipedb.cs.lmu.edu/biodb/fall2017/index.php/Week_12
  • Murata, Y., Homma, T., Kitagawa, E., Momose, Y., Sato, M. S., Odani, M., Shimizu, H., Hasegawa-Mizusawa, M., Matsumoto, R., Mizukami, S., Fujita, K., Parveen, M., Komatsu, Y., Iwahashi, H. (2006). Genome-wide expression analysis of yeast response during exposure to 4 C. Extremophiles, 10(2), 117-128.

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