Dmadere Week 11

Article Outline

1. What is the main result presented in this paper?
2. What is the importance or significance of this work?
3. What were the limitations in previous studies that led them to perform this work?
4. How did they treat the yeast cells (what experiment were they doing?)
5. What strain(s) of yeast did they use? Were the strain(s) haploid or diploid?
6. What media did they grow them in? What temperature? What type of incubator? For how long?
7. What controls did they use?
8. How many replicates did they perform per treatment or timepoint?
9. What method did they use to prepare the RNA, label it and hybridize it to the microarray?
10. What mathematical/statistical method did they use to analyze the data?
11. Are the data publicly available for download? From which web site?
12. Briefly state the result shown in each of the figures and tables, not just the ones you are presenting.
    • What do the X and Y axes represent?
    • How were the measurements made?
    • What trends are shown by the plots and what conclusions can you draw from the data?
13. How does this work compare with previous studies?
14. What are the important implications of this work?
15. What future directions should the authors take?
16. Give a critical evaluation of how well you think the authors supported their conclusions with the data they showed. Are there any major flaws to the paper?

Annotated Bibliography

1. Ibstedt, S., Sideri, T. C., Grant, C. M., & Tamás, M. J. (2014). Global analysis of protein aggregation in yeast during physiological conditions and arsenite stress. Biology open, 3(10), 913-923. doi: 10.1242/bio.20148938
2. PubMed Abstract: https://www.ncbi.nlm.nih.gov/pubmed/?term=Global+analysis+of+protein+aggregation+in+yeast+during+physiological+conditions+and+arsenite+stress
3. PubMed Central: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4197440/
4. Publisher Full Text (HTML): https://bio.biologists.org/content/3/10/913.short
5. Publisher Full Text (PDF): https://bio.biologists.org/content/biolopen/3/10/913.full.pdf
6. Copyright:2014. Published by The Company of Biologists Ltd (information found on PDF version of article)
7. Article is Open Access.
8. Article is available only online. The journal website allows for a subscription, but only for access to online content.
9. Publisher: The Company of Biologists Ltd (scientific society), non-profit, OAPA member, United Kingdom
10. The journal began in 2012.
11. The articles in the journal are peer-reviewed.
12. Editors and Board: https://bio.biologists.org/content/edboard
13. Journal Impact Factor: 1.962
14. The article is a